Endophytic Fungi of Bitter Melon \u3ci\u3e(Momordica Charantia)\u3c/i\u3e in Guangdong Province, China

Endophytic fungi can mutualistically interact with their host plants by deterring herbivores. Overall 1172 endophytic fungal isolates were recovered from roots, stems, leaves, flowers and fruits of bitter melon, Momordica charantia, at five sites in Guangdong Province. These isolates were identified to 25 genera using morphological and molecular characteristics. The endophyte communities at the five sites were similar. Alternaria alternata, Aspergillus spp., Cladosporium spp., Colletotrichum spp., Nigrospora spp., Penicillium spp., Arthrinium spp., Chaetimium spp., Curvularia spp., Fusarium spp., Phoma spp., and Phomopsis spp. were isolated from at least three of the five sites. The coefficient of similarity for endophytes ranged from 60.6% to 83.3% between any two sites. There were significant differences in the species composition of endophytes recovered from different tissues of bitter melon. Fusarium spp. was the most frequent in root and stem samples, Colletotrichum spp. in leaf samples, A. alternata in flower samples, and Cladosporium spp. in fruit samples. The coefficients of similarity for endophytes were between 42.9% and 80.0% from any two tissues. We found that the composition of endophytes of bitter melon was relatively stable across sites, but differed greatly among tissues. We also found that there were fewer insects such as aphids (Homoptera: Aphididae), leafminers (Lepidoptera, Gracillariidae), and cotton leafworms Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) collected from the leaves of bitter melon at the Huadu site compared to those collected at the Yunfu site. Whether this is related to the endophyte communities isolated from different sites requires further research

Money and Price Posting under Private Information

We study price posting with undirected search in a search-theoretic monetary model with divisible money and divisible goods. Ex ante homogeneous buyers experience match specific preference shocks in bilateral trades. The shocks follow a continuous distribution and the realization of the shocks is private information. We show that generically there exists a unique price posting monetary equilibrium. In equilibrium, each seller posts a continuous pricing schedule that exhibits quantity discounts. Buyers spend only when they have high enough preferences. As their preferences are higher, they spend more till they become cash constrained. Since inflation reduces the future purchasing power of money and the value of retaining money, buyers tend to spend their money faster in response to higher inflation. In particular, more buyers choose to spend money and buyers spend on average a higher fraction of their money. The model naturally captures the hot potato effect of inflation along both the intensive margin and the extensive margin.Economic models; Inflation and prices

One or Two Monies?

We investigate whether money constitutes a perfect substitute for the missing record-keeping technology in a quasi-linear environment, where private information and limited commitment are present. We adopt the mechanism design approach and solve a social planner�s problem subject to the resource constraint, the incentive constraints imposed by the existing frictions, and the available memory technologies. The result is that when money is divisible, concealable and in variable supply, a single money may or may not be su¢ cient to replace the record-keeping technology. We further show that two monies serve as a perfect substitute for the record-keeping technology so that there is no need for a third money

Deficiency of Mkrn2 causes abnormal spermiogenesis and spermiation, and impairs male fertility.

Although recent studies have shed insights on some of the potential causes of male infertility, new underlining molecular mechanisms still remain to be elucidated. Makorin-2 (Mkrn2) is an evolutionarily conserved gene whose biological functions are not fully known. We developed an Mrkn2 knockout mouse model to study the role of this gene, and found that deletion of Mkrn2 in mice led to male infertility. Mkrn2 knockout mice produced abnormal sperms characterized by low number, poor motility, and aberrant morphology. Disruption of Mkrn2 also caused failure of sperm release (spermiation failure) and misarrangement of ectoplasmic specialization (ES) in testes, thus impairing spermiogenesis and spermiation. To understand the molecular mechanism, we found that expression of Odf2, a vital protein in spermatogenesis, was significantly decreased. In addition, we found that expression levels of Odf2 were decreased in Mkrn2 knockout mice. We also found that MKRN2 was prominently expressed in the sperm of normal men, but was significantly reduced in infertile men. This result indicates that our finding is clinically relevant. The results of our study provided insights into a new mechanism of male infertility caused by the MKRN2 downregulation

Epimedium brevicornu Maxim extract shows protective activity against Alzheimer's disease in mice

Purpose: To investigate the protective effect of Epimedium brevicornu Maxim extract (EBME) against Alzheimer's disease in 3xTg-AD mice. Methods: The cognitive function of 3xTg-AD mice was assessed using Morris water maze test. The levels of amyloid beta deposits and NeuN in the mouse hippocampus were evaluated by immunohistochemistry. Brain neurotrophic-derived factor (BDNF) and tyrosine kinase B (TrkB) expressions were examined by western blot analysis. Results: EBME treatment significantly ameliorated learning and memory deficits in AD mice, as shown by the increased time spent in the target zone during probe tests. Compared with the 3xTg-AD mice (8.4 ± 1.1 s), the escape latency in animals treated with 600 mg/kg EBME (21.5 ± 1.1 s) was significantly increased (p < 0.01). In addition, EBME significantly decreased Aβ deposits, increased NeuN-positive cells, and upregulated the expressions of BDNF (1.5 ± 0.2, p < 0.05) and TrkB (1.6 ± 0.2, p < 0.05) in the 3xTg AD mice. Conclusion: EBME treatment may be a useful therapeutic strategy for managing memory impairment

MiRNA-145 increases therapeutic sensibility to gemcitabine treatment of pancreatic adenocarcinoma cells.

Pancreatic adenocarcinoma is one of the most leading causes of cancer-related deaths worldwide. Although recent advances provide various treatment options, pancreatic adenocarcinoma has poor prognosis due to its late diagnosis and ineffective therapeutic multimodality. Gemcitabine is the effective first-line drug in pancreatic adenocarcinoma treatment. However, gemcitabine chemoresistance of pancreatic adenocarcinoma cells has been a major obstacle for limiting its treatment effect. Our study found that p70S6K1 plays an important role in gemcitabine chemoresistence. MiR-145 is a tumor suppressor which directly targets p70S6K1 for inhibiting its expression in pancreatic adenocarcinoma, providing new therapeutic scheme. Our findings revealed a new mechanism underlying gemcitabine chemoresistance in pancreatic adenocarcinoma cells

Cross-linked CoMoO4/rGO nanosheets as oxygen reduction catalyst

Development of inexpensive and robust electrocatalysts towards oxygen reduction reaction (ORR) is crucial for the cost-affordable manufacturing of metal-air batteries and fuel cells. Here we show that cross-linked CoMoO4 nanosheets and reduced graphene oxide (CoMoO4/rGO) can be integrated in a hybrid material under one-pot hydrothermal conditions, yielding a composite material with promising catalytic activity for oxygen reduction reaction (ORR). Cyclic voltammetry (CV) and linear sweep voltammetry (LSV) were used to investigate the efficiency of the fabricated CoMoO4/rGO catalyst towards ORR in alkaline conditions. The CoMoO4/rGO composite revealed the main reduction peak and onset potential centered at 0.78 and 0.89 V (vs. RHE), respectively. This study shows that the CoMoO4/rGO composite is a highly promising catalyst for the ORR under alkaline conditions, and potential noble metal replacement cathode in fuel cells and metal-air batteries