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Characterisation of development and electrophysiological mechanisms underlying rhythmicity of the avian lymph heart
Despite significant advances in tissue engineering such as the use of scaffolds, bioreactors and pluripotent stem cells, effective cardiac tissue engineering for therapeutic purposes has remained a largely intractable challenge. For this area to capitalise on such advances, a novel approach may be to unravel the physiological mechanisms underlying the development of tissues that exhibit rhythmic contraction yet do not originate from the cardiac lineage. Considerable attention has been focused on the physiology of the avian lymph heart, a discrete organ with skeletal muscle origins yet which displays pacemaker properties normally only found in the heart. A functional lymph heart is essential for avian survival and growth in ovo. The histological nature of the lymph heart is similar to skeletal muscle although molecular and bioelectrical characterisation during development to assess mechanisms that contribute towards lymph heart contractile rhythmicity have not been undertaken. A better understanding of these processes may provide exploitable insights for therapeutic rhythmically contractile tissue engineering approaches in this area of significant unmet clinical need. Here, using molecular and electrophysiological approaches, we describe the molecular development of the lymph heart to understand how this skeletal muscle becomes fully functional during discrete in ovo stages of development. Our results show that the lymph heart does not follow the normal transitional programme of myogenesis as documented in most skeletal muscle, but instead develops through a concurrent programme of precursor expansion, commitment to myogenesis and functional differentiation which offers a mechanistic explanation for its rapid development. Extracellular electrophysiological field potential recordings revealed that the peak-to-peak amplitude of electrically evoked local field potentials elicited from isolated lymph heart were significantly reduced by treatment with carbachol; an effect that could be fully reversed by atropine. Moreover, nifedipine and cyclopiazonic acid both significantly reduced peak-to-peak local field potential amplitude. Optical recordings of lymph heart showed that the organ’s rhythmicity can be blocked by the HCN channel blocker, ZD7288; an effect also associated with a significant reduction in peak-to-peak local field potential amplitude. Additionally, we also show that isoforms of HCN channels are expressed in avian lymph heart. These results demonstrate that cholinergic signalling and L-type Ca2+ channels are important in excitation and contraction coupling, while HCN channels contribute to maintenance of lymph heart rhythmicity
Cyclic Peptides as TType Calcium Channel Blockers: Characterization and Molecular Mapping of the Binding Site
DEVELOPMENT OF MAMMALIAN NERVE MUSCLE SYNAPSES IN CULTURE - LACK OF INTERFERENCE BY ANTIBODIES TO THE NEURAL CELL-ADHESION MOLECULE N-CAM AND ITS L2/HNK-1 CARBOHYDRATE EPITOPE
MEHRKE G, Jockusch H, SCHACHNER M. DEVELOPMENT OF MAMMALIAN NERVE MUSCLE SYNAPSES IN CULTURE - LACK OF INTERFERENCE BY ANTIBODIES TO THE NEURAL CELL-ADHESION MOLECULE N-CAM AND ITS L2/HNK-1 CARBOHYDRATE EPITOPE. NEUROSCIENCE LETTERS. 1987;78(3):247-252
Beating heart muscle in a skeletal muscle bed.
Jockusch H, MEHRKE G, FUCHTBAUER EM. BEATING HEART-MUSCLE IN A SKELETAL-MUSCLE BED. EXPERIMENTAL NEUROLOGY. 1983;81(3):749-755
Differential calcium channel‐mediated dopaminergic modulation in the subthalamonigral synapse
SYNAPSE FORMATION AND SYNAPTIC ACTIVITY IN MAMMALIAN NERVE-MUSCLE CO-CULTURE ARE NOT INHIBITED BY ANTIBODIES TO NEURAL CELL-ADHESION MOLECULE L1
MEHRKE G, Jockusch H, FAISSNER A, SCHACHNER M. SYNAPSE FORMATION AND SYNAPTIC ACTIVITY IN MAMMALIAN NERVE-MUSCLE CO-CULTURE ARE NOT INHIBITED BY ANTIBODIES TO NEURAL CELL-ADHESION MOLECULE L1. NEUROSCIENCE LETTERS. 1984;44(3):235-239