Seroepidemiological and biomolecular survey on

Toxoplasma gondii is a zoonotic parasite able of infecting all warm-blooded animals. Toxoplasmosis is one of the major foodborne diseases globally. The consumption of wild boar (Sus scrofa) meat from recreational hunting has been linked to outbreaks of human toxoplasmosis. The island of Sardinia (Italy) contains a large wild boar population, thus providing an opportunity to assess the distribution of Toxoplasma in this species and the associated risks of transmission to humans. A total of 562 wild boars were screened: heart and meat juice samples were tested for T. gondii DNA via nested-PCR and IgG anti-Toxoplasma by commercial ELISA. Anti-Toxoplasma IgG were detected in 24.6% (138/562) of animals, while 37.2% (209/562) of the heart samples were PCR positive. The prevalence of T. gondii antibodies and DNA highlights the potential role of wild boar as an important reservoir for this parasite. The study suggests that wild boar could play a significant role in spreading the parasite to humans. As wild boar numbers are increasing throughout their range, their potential role in transmitting toxoplasmosis should be communicated to stakeholders, and the impact of different population control methods on disease transmission should be thoroughly assessed to mitigate potential threats effectively

Assessment of Trace Metal and Metalloid Accumulation and Human Health Risk from Vegetables Consumption through Spinach and Coriander Specimens Irrigated with Wastewater

This study focused on evaluating the metal and metalloid contamination and associated risks in the two vegetables crops, coriander (Coriandrum sativum) and spinach (Spinacia oleracea) treated with three water regimes, canal water, groundwater and municipal wastewater. These vegetables are widely consumed by people and are also used in traditional medicine for treating various disorders. Metal and metalloid accumulation (Zn, Pb, Se, Cu, As, Mo, Fe, Ni) was found higher in vegetables treated with wastewater. Wastewater treated soil had high pollution load index. Fe, Zn, As and Pb had higher values in water, soil and vegetables as compared to other studied metals. Overall, metal correlation for soil and vegetables was significant and positive except for Fe and Cu in spinach. The highest value for daily metal intake was estimated for Fe while Se had the lowest value for the same index. It was thus concluded that trace metal and metalloid accumulation was a major health concern for the public consuming these vegetables

Potential Toxic Metal Accumulation in Soil, Forage and Blood Plasma of Buffaloes Sampled from Jhang, Pakistan

This study was conducted to determine the concentration of toxic metals in soil, forage and blood plasma of lactating and non-lactating buffaloes in the district Jhang, Punjab, Pakistan. Soil samples were collected from varying distances from the road side. Plasma separation was achieved by centrifugation. The concentration of arsenic (As), selenium (Se), cadmium (Cd), chromium (Cr), iron (Fe), zinc (Zn), copper (Cu) and cobalt (Co) were determined by using Atomic Absorption Spectrophotometer. The results of the study showed that the mean As, Se and Cd concentrations in soil samples were lower while Cr, Fe, Zn, Cu and Co were higher than the official guidelines. In plasma samples, mean concentration values of Co, Zn, Fe, Cd, Se and As were lower while Cu and Cr were higher than the recommended concentrations. According to the results of the study there was no potential exposure of toxicity in buffaloes of the study area

The Effects of Irrigation with Diverse Wastewater Sources on Heavy Metal Accumulation in Kinnow and Grapefruit Samples and Health Risks from Consumption

This study aimed to assess the concentrations of Pb, Cr, Cd, and Ni in the edible parts of grapefruit and kinnow fruit irrigated with sewage water, tube-well water, and canal water. Preparation of the samples used in the study for metal analysis was carried out by the wet acid digestion method. Atomic absorption spectrometry was used for metal determination. All of the studied metals were more abundant in water, soil, and fruit samples from sites irrigated with sewage water than from those irrigated with tube-well and canal water. The concentrations of Pb were established in the range of 0.047 to 0.175 mg/L in water, 12.652 to 47.863 mg/kg in soil, and 0.023 to 0.124 mg/kg in fruit samples of both varieties. The range of Cr varied from 0.107 to 0.741 mg/L in water, from 1.121 to 5.835 mg/kg in soil, and from 0.054 to 0.784 mg/kg in fruit samples of both varieties. The range of Cd varied from 0.004 to 0.028 mg/L in water, from 1.258 to 4.246 mg/kg in soil, and from 0.012 to 0.131 mg/kg in fruit samples of both varieties. The range of Ni varied from 0.384 to 1.815 mg/L in water, from 5.367 to 18.658 mg/kg in soil, and from 0.028 to 1.475 mg/kg in fruit samples of both varieties. The pollution load index indicated that Pb exceeded a value of 1, while Cd and Ni exceeded 1 only in sewage-water-irrigated sites. The bioconcentration factor, enrichment factor, daily intake of metal, and health risk index values for all metals were below 1, within permissible limits, indicating no immediate health risks associated with consuming these fruits

Evaluation of nickel toxicity and potential health implications of agriculturally diversely irrigated wheat crop varieties

Nickel (Ni) is essential for plant metabolism but is dangerous to most plants and other organisms when present in large concentrations. In this study, nickel (Ni) was chosen as an important environmental pollutant and toxic substance due to its widespread availability, and high persistence, causing soil pollution and serious environmental problems. The main aim of the study is to determine the Ni content in various wheat varieties and soil grown in various water regimes (sewage water, groundwater and industrial water) in Punjab, Pakistan. The Ni concentrations were determined via the ecological environment (water, soils) and below (roots) and above-ground plant organs (shoots and wheat grains) and phytostabilization potential assessment using various pollution indices. The results showed that the mean Ni concentration in different wheat varieties varied from 1.35 to 2.45 mg/kg, 1.17 to 2.42 mg/kg, 1.09 to 2.10 mg/kg and 0.99 to 1.54 mg/kg in soil, root, shoot, and grains, respectively. Research findings also revealed that the nickel concentration in soil and grains is within the safe limits and all the indices for Ni are less than 1. As a result, the studied wheat cultivars appear to be safe for consumption. However, it can be said that the continuous use of wastewater for irrigation may pose health risks for living things in the food chain

Seroepidemiological and biomolecular survey on Toxoplasma gondii in Sardinian wild boar (Sus scrofa).

Toxoplasma gondii is a zoonotic parasite able of infecting all warm-blooded animals. Toxoplasmosis is one of the major foodborne diseases globally. The consumption of wild boar (Sus scrofa) meat from recreational hunting has been linked to outbreaks of human toxoplasmosis. The island of Sardinia (Italy) contains a large wild boar population, thus providing an opportunity to assess the distribution of Toxoplasma in this species and the associated risks of transmission to humans. A total of 562 wild boars were screened: heart and meat juice samples were tested for T. gondii DNA via nested-PCR and IgG anti-Toxoplasma by commercial ELISA. Anti-Toxoplasma IgG were detected in 24.6% (138/562) of animals, while 37.2% (209/562) of the heart samples were PCR positive. The prevalence of T. gondii antibodies and DNA highlights the potential role of wild boar as an important reservoir for this parasite. The study suggests that wild boar could play a significant role in spreading the parasite to humans. As wild boar numbers are increasing throughout their range, their potential role in transmitting toxoplasmosis should be communicated to stakeholders, and the impact of different population control methods on disease transmission should be thoroughly assessed to mitigate potential threats effectively

Evaluation of Cu, Zn, Fe, and Mn Concentrations in Water, Soil, and Fruit Samples in Sargodha District, Pakistan

This study aimed to assess the concentrations of copper (Cu), zinc (Zn), iron (Fe), and manganese (Mn) in the edible parts of grapefruit and kinnow fruit irrigated with sewage water (SW), tube-well water (TW), and canal water (CW). Preparation of the samples used in the study for metal analysis was carried out via the wet acid digestion method. Atomic absorption spectrometry (AAS) was used for metal determination. According to the results, Cu concentration ranged from 0.152 to 0.754 mg/L in water, 5.254 to 41.659 mg/kg in soil, and 0.128 to 0.864 mg/kg in fruit samples. Zn concentration varied from 0.574 to 2.723 mg/L in water, 17.812 to 112.954 mg/kg in soil, and 2.658 to 42.642 mg/kg in fruit samples. Fe concentration ranged from 0.254 to 1.245 mg/L in water, 10.635 to 48.638 mg/kg in soil, and 1.062 to 7.584 mg/kg in fruit samples. Mn concentration ranged from 0.154 to 0.638 mg/L in water, 51.283 to 183.865 mg/kg in soil, and 0.136 to 1.464 mg/kg in fruit samples. The Pollution Load Index (PLI) indicated that Cu and Mn exceeded a PLI value of one, and Zn had a PLI > 1 only in sewage water-irrigated sites. Bioconcentration Factor (BCF), Enrichment Factor (EF), Daily Intake of Metal (DIM), and Health Risk Index (HRI) values for all metals were within permissible limits, indicating no immediate health risks associated with consuming these fruits

Risk Assessment of Heavy Metals in Basmati Rice: Implications for Public Health

Basmati rice is increasingly recognized and consumed in different parts of the world due to its different tastes and nutritional properties. This research focused on determining the cadmium (Cd), cobalt (Co), Copper (Cu), iron (Fe), manganese (Mn), nickel (Ni) and zinc (Zn) content in locally grown basmati rice in Pakistan and assessing the risks of these values to human health. Root, shoot and grain samples of basmati rice were taken, along with soil samples from the five regions studied. Metal mean concentrations (mg/kg) in grains fluctuated from 2.70 to 9.80 for Cd, 4.80 to 9.85 for Zn, 1.16 to 1.46 for Cu, 1.84 to 10.86 for Co, 2.05 to 13.07 for Fe, 5.03 to 11.11 for Mn and 3.24 to 13.28 for Ni, respectively. All metal values were within permissible limits except for Cd. The enrichment factor for Cd was highest among all sites. Cobalt and zinc had the highest bioaccumulation factor and translocation factor. The highest enrichment factor was noticed for Cd and the lowest for Cu. The health risk index at all examined sites was less than one. Consistent examination is recommended to limit health hazards instigated by the use of rice polluted with a greater concentration of Cd