FORAGE QUALITY TRAITS OF SOME ASTERACEAE FAMILY SPECIES FOUND IN NATURAL FLORA OF SOUTHEASTERN ANATOLIA

This study was carried out in order to reveal the forage quality traits of twelve Asteraceae family species found in Natural Flora of Southeastern Anatolia in terms of animal nutrition. For this reason, forage samples of the species were taken with three replications during flowering periods of the plant species. According to statistical analysis results, there found highly significant (P<0.01) differences among the species in terms of all of the investigated traits. And the following ranges were determined among the species in the investigated traits; crude protein content (CP) 13.37%-26.90%, acid detergent fiber (ADF) content 21.78%-34.17, neutral detergent fiber (NDF) content 28.69%-40.52%, digestible dry matter (DDM) content 62.28%-71.94%, dry matter intake (DMI) 2.97%-4.21%, metabolizable energy (ME) 9.57-11.45 MJ/kg, relative feed value (RFV) 144.2-230.4, phosphor (P) content 0.27%-0.40%, potassium (K) content 1.97%-5.42, calcium (Ca) content 1.21%-1.78%, magnesium (Mg) content 0.32%-0.49%, Ca/P ratio 3.25-5.19 and K/(Ca+Mg) ratio 1.19-2.84. Result of the study revealed that Notobasis syriaca (L.) Cass. and Onopardum acontium L. species by far the highest CP content, and except for Gundelia tournoforti L. var. armata all of the examined species had the best forage quality, Prime Forage Class, by considering their RFV values. Moreover, it was determined that the macro mineral contents of the species were above the recommended values for livestock feeding. Additionally, it was determined that most of the examined species had below critical grass tetany value (2.2), away from risk of the grass tetany disorder. Finally, due to Ca/P ratio of the species well above recommended level, the danger of Milk Fewer or Hypocalcaemia disease should be taken into account when using forages of the Asteraceae family species in animal feed

Twist/Writhe Partitioning in a Coarse-Grained DNA Minicircle Model

Here we present a systematic study of supercoil formation in DNA minicircles under varying linking number by using molecular dynamics simulations of a two-bead coarse-grained model. Our model is designed with the purpose of simulating long chains without sacrificing the characteristic structural properties of the DNA molecule, such as its helicity, backbone directionality and the presence of major and minor grooves. The model parameters are extracted directly from full-atomistic simulations of DNA oligomers via Boltzmann inversion, therefore our results can be interpreted as an extrapolation of those simulations to presently inaccessible chain lengths and simulation times. Using this model, we measure the twist/writhe partitioning in DNA minicircles, in particular its dependence on the chain length and excess linking number. We observe an asymmetric supercoiling transition consistent with experiments. Our results suggest that the fraction of the linking number absorbed as twist and writhe is nontrivially dependent on chain length and excess linking number. Beyond the supercoiling transition, chains of the order of one persistence length carry equal amounts of twist and writhe. For longer chains, an increasing fraction of the linking number is absorbed by the writhe.Comment: 21 pages, 7 figures, 1 tabl

THE EFFECT OF DIFFERENT CUTTING TIMES ON THE MACRO MINERAL CONTENT OF ALFALFA (MEDICAGO SATIVA L.) GENOTYPES

This study was conducted to determine the effect of different cutting times on the important macro mineral contents of twenty-four alfalfa (Medicago sativa L.) genotypes. For this reason, a field trial was established according to separated plots in randomized blocks design with three replications in the Research Area of Agriculture Faculty of Dicle University, Diyarbakır, Turkey in 2020. The alfalfa genotypes were subjected to three different forage cutting times. The 1st, 2nd and 3rd cuttings were made respectively in the pre-flowering, 10% flowering and full flowering periods of alfalfa genotypes. In terms of all examined macro minerals in the study, there found to be statistically highly significant (P<0.01) differences between cutting times, genotypes and genotypes × cutting times interaction. In the research, the macro mineral contents of the genotypes changed between the cutting times as follows; calcium (Ca) 1.41%-1.81%, phosphorus (P) 0.37%-0.50%, magnesium (Mg) 0.31%-0.42% and potassium (K) 1.94%-3.42%. The results of the research revealed that Ca and Mg contents in forages of alfalfa genotypes increased with advancing plant maturity stage, whereas K and P contents decreased. In conclusion, according to the average of the cutting times, Resis (22) cultivar came to fore in terms of Ca content, while many genotypes, sharing the same statistical group, were found superior in terms of P, Mg and K contents

Modeling Adsorption, Conformation, and Orientation of the Fis1 Tail Anchor at the Mitochondrial Outer Membrane

Proteins can be targeted to organellar membranes by using a tail anchor (TA), a stretch of hydrophobic amino acids found at the polypeptide carboxyl-terminus. The Fis1 protein (Fis1p), which promotes mitochondrial and peroxisomal division in the yeast Saccharomyces cerevisiae, is targeted to those organelles by its TA. Substantial evidence suggests that Fis1p insertion into the mitochondrial outer membrane can occur without the need for a translocation machinery. However, recent findings raise the possibility that Fis1p insertion into mitochondria might be promoted by a proteinaceous complex. Here, we have performed atomistic and coarse-grained molecular dynamics simulations to analyze the adsorption, conformation, and orientation of the Fis1(TA). Our results support stable insertion at the mitochondrial outer membrane in a monotopic, rather than a bitopic (transmembrane), configuration. Once inserted in the monotopic orientation, unassisted transition to the bitopic orientation is expected to be blocked by the highly charged nature of the TA carboxyl-terminus and by the Fis1p cytosolic domain. Our results are consistent with a model in which Fis1p does not require a translocation machinery for insertion at mitochondria

Modeling Adsorption, Conformation, and Orientation of the Fis1 Tail Anchor at the Mitochondrial Outer Membrane

Proteins can be targeted to organellar membranes by using a tail anchor (TA), a stretch of hydrophobic amino acids found at the polypeptide carboxyl-terminus. The Fis1 protein (Fis1p), which promotes mitochondrial and peroxisomal division in the yeast Saccharomyces cerevisiae, is targeted to those organelles by its TA. Substantial evidence suggests that Fis1p insertion into the mitochondrial outer membrane can occur without the need for a translocation machinery. However, recent findings raise the possibility that Fis1p insertion into mitochondria might be promoted by a proteinaceous complex. Here, we have performed atomistic and coarse-grained molecular dynamics simulations to analyze the adsorption, conformation, and orientation of the Fis1(TA). Our results support stable insertion at the mitochondrial outer membrane in a monotopic, rather than a bitopic (transmembrane), configuration. Once inserted in the monotopic orientation, unassisted transition to the bitopic orientation is expected to be blocked by the highly charged nature of the TA carboxyl-terminus and by the Fis1p cytosolic domain. Our results are consistent with a model in which Fis1p does not require a translocation machinery for insertion at mitochondria

Ekonomik büyüme ve borsa getirisi arasındaki ilişki: Türkiye örneği

Financial development is one of the most important determinants of the economic development. Financial developments in Turkey began in the early 1980s and still have continued. During this period, it has survived a severe interaction between financial development and economic growth. In this study, the causality relationship between stock returns and economic growth in Turkey it was analysed over the period 1998Q2-2014Q2. In this context; firstly, Bootstrapped Toda-Yamamoto and Frequency Domain causality tests were applied in order to understand the causality relationship between the two variables. As a result of the Bootstrapped Toda-Yamamoto causality test results, there is no relation of causality between the variables, but according to Frequency Domain causality test stock returns cause the economic growth in short term and economic growth cause stock returns in medium term.Finansal gelişme ekonomik kalkınmanın en önemli belirleyicilerinden birisidir. Türkiye’de finansal gelişme 1980’li yılların başında başlamış ve halen de devam etmektedir. Geçen süre içerisinde finansal gelişme ile ekonomik kalkınma arasında ciddi bir etkileşim yaşanagelmiştir. Bu çalışmada, Türkiye’de 1998Q2-2014Q2 döneminde borsa getirisi ve ekonomik büyüme arasındaki ilişki incelenmiştir. Bu bağlamda, iki değişken arasındaki nedensellik ilişkisini tespit etmek amacıyla Bootstrap tabanlı Toda-Yamamoto ve frekans dağılımı nedensellik testleri uygulanmıştır. Bootstrap tabanlı Toda-Yamamoto nedensellik testi sonuçlarına göre değişkenler arasında nedensellik ilişkisi bulunmamaktadır. Fakat, frekans dağılımı nedensellik testi göstermektedir ki; borsa getirisi kısa dönemde ekonomik büyümenin ve ekonomik büyüme de orta dönemde borsa getirisinin nedenidir

P2X7 receptor activates multiple selective dye-permeation pathways

ABSTRACT P2X7 receptor has gained an increasing importance as a drug target. One important response to P2X7 receptor stimulation is the uptake of large molecular weight tracers into cells. However, mechanism for this response is not understood clearly, but it is generally believed that a nonselective large pore protein forms this P2X7 receptor-activated permeability pathway. We examined human embryonic kidney (HEK) 293 cells transfected with rat P2X7 receptors (HEK-rP2X7) and a macrophage derived cell line, RAW 264.7, that expresses an endogenous P2X7 receptor. We used confocal microscopy to investigate uptake of different types of dyes into these cells after ATP application. Stimulation of P2X7 receptors in HEK-rP2X7 cells activated two different dye uptake pathways. The first was permeable to the cationic fluorescent dyes YO-PRO-1 and TO-TO-1 but not to the anionic dyes lucifer yellow and calcein and did not require intracellular Ca 2ϩ concentration ([Ca 2ϩ ] i ) increase to be activated. The second pathway permeated only lucifer yellow and was completely dependent on [Ca 2ϩ ] i for activation. In RAW 264.7 cells, P2X7 receptor stimulation activated uptake of ethidium, YO-PRO-1, TO-TO-1, lucifer yellow, and calcein. Again, two different permeation pathways were discerned in RAW 264.7 cells: one permeated only ethidium and the other one, only lucifer yellow. We did observed no clear [Ca 2ϩ ] i dependence for these permeation pathways. Our results demonstrate that instead of a single nonselective pore, P2X7 receptor seems to activate at least two permeation pathways, one for cationic and one for anionic dyes with different activation properties. The P2X7 receptor is a member of P2X receptor family, which is composed of ligand-gated ion channels. Activated P2X7 receptor causes not only a cationic membrane current, but also permeabilization of the cell membrane to large molecular weight molecules P2X7 receptors are known to be important in the pathophysiology of arthritis and mediation of pain (for review, see Article, publication date, and citation information can be found a