824 research outputs found
Thermal Infrared Multispectral Scanner (TIMS): An investigator's guide to TIMS data
The Thermal Infrared Multispectral Scanner (TIMS) is a NASA aircraft scanner providing six channel spectral capability in the thermal infrared region of the electromagnetic spectrum. Operating in the atmospheric window region (8 to 12 micrometers) with a channel sensitivity of approximately 0.1 C, TIMS may be used whenever an accurate measure of the Earth's surface is needed. A description of this scanner is provided as well as a discussion of data acquisition and reduction
Tracking and data systems support for the Helios project. Volume 2: DSN support of Project Helios April 1975 - May 1976
Deep Space Network activities in the development of the Helios B mission from planning through entry of Helios 2 into first superior conjunction (end of Mission Phase II) are summarized. Network operational support activities for Helios 1 from first superior conjunction through entry into third superior conjunction are included
Characterization of Genes Encoding Poly(A) Polymerases in Plants: Evidence for Duplication and Functional Specialization
BACKGROUND:Poly(A) polymerase is a key enzyme in the machinery that mediates mRNA 3' end formation in eukaryotes. In plants, poly(A) polymerases are encoded by modest gene families. To better understand this multiplicity of genes, poly(A) polymerase-encoding genes from several other plants, as well as from Selaginella, Physcomitrella, and Chlamydomonas, were studied. METHODOLOGY/PRINCIPAL FINDINGS:Using bioinformatics tools, poly(A) polymerase-encoding genes were identified in the genomes of eight species in the plant lineage. Whereas Chlamydomonas reinhardtii was found to possess a single poly(A) polymerase gene, other species possessed between two and six possible poly(A) polymerase genes. With the exception of four intron-lacking genes, all of the plant poly(A) polymerase genes (but not the C. reinhardtii gene) possessed almost identical intron positions within the poly(A) polymerase coding sequences, suggesting that all plant poly(A) polymerase genes derive from a single ancestral gene. The four Arabidopsis poly(A) polymerase genes were found to be essential, based on genetic analysis of T-DNA insertion mutants. GFP fusion proteins containing three of the four Arabidopsis poly(A) polymerases localized to the nucleus, while one such fusion protein was localized in the cytoplasm. The fact that this latter protein is largely pollen-specific suggests that it has important roles in male gametogenesis. CONCLUSIONS/SIGNIFICANCE:Our results indicate that poly(A) polymerase genes have expanded from a single ancestral gene by a series of duplication events during the evolution of higher plants, and that individual members have undergone sorts of functional specialization so as to render them essential for plant growth and development. Perhaps the most interesting of the plant poly(A) polymerases is a novel cytoplasmic poly(A) polymerase that is expressed in pollen in Arabidopsis; this is reminiscent of spermatocyte-specific cytoplasmic poly(A) polymerases in mammals
Effect of non-zero constant vorticity on the nonlinear resonances of capillary water waves
The influence of an underlying current on 3-wave interactions of capillary
water waves is studied. The fact that in irrotational flow resonant 3-wave
interactions are not possible can be invalidated by the presence of an
underlying current of constant non-zero vorticity. We show that: 1) wave trains
in flows with constant non-zero vorticity are possible only for two-dimensional
flows; 2) only positive constant vorticities can trigger the appearance of
three-wave resonances; 3) the number of positive constant vorticities which do
trigger a resonance is countable; 4) the magnitude of a positive constant
vorticity triggering a resonance can not be too small.Comment: 6 pages, submitte
Doubly connected minimal surfaces and extremal harmonic mappings
The concept of a conformal deformation has two natural extensions:
quasiconformal and harmonic mappings. Both classes do not preserve the
conformal type of the domain, however they cannot change it in an arbitrary
way. Doubly connected domains are where one first observes nontrivial conformal
invariants. Herbert Groetzsch and Johannes C. C. Nitsche addressed this issue
for quasiconformal and harmonic mappings, respectively. Combining these
concepts we obtain sharp estimates for quasiconformal harmonic mappings between
doubly connected domains. We then apply our results to the Cauchy problem for
minimal surfaces, also known as the Bjorling problem. Specifically, we obtain a
sharp estimate of the modulus of a doubly connected minimal surface that
evolves from its inner boundary with a given initial slope.Comment: 35 pages, 2 figures. Minor edits, references adde
Geophysical Research
Contains research objectives and reports on two research projects.Joint Services Electronics Programs (U. S. Army, U. S. Navy, and U. S. Air Force) under Contract DA 36-039-AMC-03200(E)National Aeronautics and Space Administration (Grant NGR-22-009-131)Joint Services Electronics Programs (Contract DA 36-039-AMC-03200(E)
Parabolic stable surfaces with constant mean curvature
We prove that if u is a bounded smooth function in the kernel of a
nonnegative Schrodinger operator on a parabolic Riemannian
manifold M, then u is either identically zero or it has no zeros on M, and the
linear space of such functions is 1-dimensional. We obtain consequences for
orientable, complete stable surfaces with constant mean curvature
in homogeneous spaces with four
dimensional isometry group. For instance, if M is an orientable, parabolic,
complete immersed surface with constant mean curvature H in
, then and if equality holds, then
M is either an entire graph or a vertical horocylinder.Comment: 15 pages, 1 figure. Minor changes have been incorporated (exchange
finite capacity by parabolicity, and simplify the proof of Theorem 1)
Physics in Riemann's mathematical papers
Riemann's mathematical papers contain many ideas that arise from physics, and
some of them are motivated by problems from physics. In fact, it is not easy to
separate Riemann's ideas in mathematics from those in physics. Furthermore,
Riemann's philosophical ideas are often in the background of his work on
science. The aim of this chapter is to give an overview of Riemann's
mathematical results based on physical reasoning or motivated by physics. We
also elaborate on the relation with philosophy. While we discuss some of
Riemann's philosophical points of view, we review some ideas on the same
subjects emitted by Riemann's predecessors, and in particular Greek
philosophers, mainly the pre-socratics and Aristotle. The final version of this
paper will appear in the book: From Riemann to differential geometry and
relativity (L. Ji, A. Papadopoulos and S. Yamada, ed.) Berlin: Springer, 2017
Arabidopsis mRNA polyadenylation machinery: comprehensive analysis of protein-protein interactions and gene expression profiling
BACKGROUND: The polyadenylation of mRNA is one of the critical processing steps during expression of almost all eukaryotic genes. It is tightly integrated with transcription, particularly its termination, as well as other RNA processing events, i.e. capping and splicing. The poly(A) tail protects the mRNA from unregulated degradation, and it is required for nuclear export and translation initiation. In recent years, it has been demonstrated that the polyadenylation process is also involved in the regulation of gene expression. The polyadenylation process requires two components, the cis-elements on the mRNA and a group of protein factors that recognize the cis-elements and produce the poly(A) tail. Here we report a comprehensive pairwise protein-protein interaction mapping and gene expression profiling of the mRNA polyadenylation protein machinery in Arabidopsis.
RESULTS: By protein sequence homology search using human and yeast polyadenylation factors, we identified 28 proteins that may be components of Arabidopsis polyadenylation machinery. To elucidate the protein network and their functions, we first tested their protein-protein interaction profiles. Out of 320 pair-wise protein-protein interaction assays done using the yeast two-hybrid system, 56 (approximately 17%) showed positive interactions. 15 of these interactions were further tested, and all were confirmed by co-immunoprecipitation and/or in vitro co-purification. These interactions organize into three distinct hubs involving the Arabidopsis polyadenylation factors. These hubs are centered around AtCPSF100, AtCLPS, and AtFIPS. The first two are similar to complexes seen in mammals, while the third one stands out as unique to plants. When comparing the gene expression profiles extracted from publicly available microarray datasets, some of the polyadenylation related genes showed tissue-specific expression, suggestive of potential different polyadenylation complex configurations.
CONCLUSION: An extensive protein network was revealed for plant polyadenylation machinery, in which all predicted proteins were found to be connecting to the complex. The gene expression profiles are indicative that specialized sub-complexes may be formed to carry out targeted processing of mRNA in different developmental stages and tissue types. These results offer a roadmap for further functional characterizations of the protein factors, and for building models when testing the genetic contributions of these genes in plant growth and development
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