Speculations About the Effects of Fire and Lava Flows on Human Evolution

Recent research argues that an association with fire, stretching back millions of years, played a central role in human evolution resulting in many modern human adaptations. Others argue that hominin evolution was driven by the roughness of topographic features that resulted from tectonic activity in the African Rift valley. I combine these hypotheses to propose that, for millions of years, active lava flows in the African Rift provided consistent but isolated sources of fire, providing very specific adaptive pressures and opportunities to small isolated groups of hominins. This allowed these groups of early hominins to develop many fire specific adaptations such as bipedalism, smaller teeth and mouths, shorter intestines, larger brains, and perhaps a host of social adaptations. By about 1.8 million years ago, Homo erectus emerged as a fire adapted species and mastered the technology necessary to make fire itself. This technology allowed them to move into the rest of the world, taking a new kind of fire with them that would change ecosystems everywhere they went. This hypothesis is supported by recent geologic work that describes a large lava flow occurring in the region of the Olduvai Gorge during the 200 000 year time period we believe Homo erectus emerged in the area

Macaw Cam: Exploratory Camera Trap Techniques for Monitoring and Conservation of Scarlet Macaw (Ara macao) Nests

In this study, we explored new, low-cost camera trap techniques to monitor Scarlet Macaws in one of their last two self-sustaining habitats in Costa Rica. Camera trap monitors have begun to produce new insights in avian research and we use them not only because Macaws are threatened, but their imagery can be used to enhance the public’s understanding of the connections between science and conservation efforts. We mounted camera units on two trees with nesting Macaws in Costa Rica’s Carara National Park and monitored one nest remotely for seven consecutive months

A New Method Comparing Snowmelt Timing with Annual Area Burned

The interactions between climate and wildland fire are complex. To better understand these interactions, we used ArcMap 10.2.2 to examine the relationships between early spring snowmelt and total annual area burned within a defined region of the Rocky Mountains of the western United States. Our research methods used Monitoring Trends in Burn Severity (MTBS) fire perimeter data and weekly snow extent provided by the Rutgers Global Snow Lab analysis of National Oceanic and Atmospheric Administration (NOAA) daily snow maps. Our results indicated a significant correlation between early spring snowmelt and total annual area burned (P = 0.0497), providing further evidence that snowmelt timing may be a driving factor for wildland fires. This project builds on the findings of previous studies and provides a novel method for making general predictions about the upcoming fire season months in advance, using freely available remotely sensed data in real time. Further research should apply our model to a broader geographic area, and incorporate higher resolution snowmelt timing data

Transcriptional and immunohistological assessment of immune infiltration in pancreatic cancer.

Pancreatic adenocarcinoma is characterized by a complex tumor environment with a wide diversity of infiltrating stromal and immune cell types that impact the tumor response to conventional treatments. However, even in this poorly responsive tumor the extent of T cell infiltration as determined by quantitative immunohistology is a candidate prognostic factor for patient outcome. As such, even more comprehensive immunophenotyping of the tumor environment, such as immune cell type deconvolution via inference models based on gene expression profiling, holds significant promise. We hypothesized that RNA-Seq can provide a comprehensive alternative to quantitative immunohistology for immunophenotyping pancreatic cancer. We performed RNA-Seq on a prospective cohort of pancreatic tumor specimens and compared multiple approaches for gene expression-based immunophenotyping analysis compared to quantitative immunohistology. Our analyses demonstrated that while gene expression analyses provide additional information on the complexity of the tumor immune environment, they are limited in sensitivity by the low overall immune infiltrate in pancreatic cancer. As an alternative approach, we identified a set of genes that were enriched in highly T cell infiltrated pancreatic tumors, and demonstrate that these can identify patients with improved outcome in a reference population. These data demonstrate that the poor immune infiltrate in pancreatic cancer can present problems for analyses that use gene expression-based tools; however, there remains enormous potential in using these approaches to understand the relationships between diverse patterns of infiltrating cells and their impact on patient treatment outcomes

Oxytocin Signaling in Mouse Taste Buds

The neuropeptide, oxytocin (OXT), acts on brain circuits to inhibit food intake. Mutant mice lacking OXT (OXT knockout) overconsume salty and sweet (i.e. sucrose, saccharin) solutions. We asked if OXT might also act on taste buds via its receptor, OXTR.Using RT-PCR, we detected the expression of OXTR in taste buds throughout the oral cavity, but not in adjacent non-taste lingual epithelium. By immunostaining tissues from OXTR-YFP knock-in mice, we found that OXTR is expressed in a subset of Glial-like (Type I) taste cells, and also in cells on the periphery of taste buds. Single-cell RT-PCR confirmed this cell-type assignment. Using Ca2+ imaging, we observed that physiologically appropriate concentrations of OXT evoked [Ca2+]i mobilization in a subset of taste cells (EC50 approximately 33 nM). OXT-evoked responses were significantly inhibited by the OXTR antagonist, L-371,257. Isolated OXT-responsive taste cells were neither Receptor (Type II) nor Presynaptic (Type III) cells, consistent with our immunofluorescence observations. We also investigated the source of OXT peptide that may act on taste cells. Both RT-PCR and immunostaining suggest that the OXT peptide is not produced in taste buds or in their associated nerves. Finally, we also examined the morphology of taste buds from mice that lack OXTR. Taste buds and their constituent cell types appeared very similar in mice with two, one or no copies of the OXTR gene.We conclude that OXT elicits Ca2+ signals via OXTR in murine taste buds. OXT-responsive cells are most likely a subset of Glial-like (Type I) taste cells. OXT itself is not produced locally in taste tissue and is likely delivered through the circulation. Loss of OXTR does not grossly alter the morphology of any of the cell types contained in taste buds. Instead, we speculate that OXT-responsive Glial-like (Type I) taste bud cells modulate taste signaling and afferent sensory output. Such modulation would complement central pathways of appetite regulation that employ circulating homeostatic and satiety signals

Activating the Nucleic Acid-Sensing Machinery for Anticancer Immunity.

Nucleic acid sensing pathways have likely evolved as part of a broad pathogen sensing strategy intended to discriminate infectious agents and initiate appropriate innate and adaptive controls. However, in the absence of infectious agents, nucleic acid sensing pathways have been shown to play positive and negative roles in regulating tumorigenesis, tumor progression and metastatic spread. Understanding the normal biology behind these pathways and how they are regulated in malignant cells and in the tumor immune environment can help us devise strategies to exploit nucleic acid sensing to manipulate anti-cancer immunity