Latrunculin A delays anaphase onset in fission yeast by disrupting an ase1-independent pathway controlling mitotic spindle stability

It has been proposed previously that latrunculin A, an inhibitor of actin polymerization, delays the onset of anaphase by causing spindle misorientation in fission yeast. However, we show that {Delta}mto1 cells, which are defective in nucleation of cytoplasmic microtubules, have profoundly misoriented spindles but are not delayed in the timing of sister chromatid separation, providing compelling evidence that fission yeast does not possess a spindle orientation checkpoint. Instead, we show that latrunculin A delays anaphase onset by disrupting interpolar microtubule stability. This effect is abolished in a latrunculin A-insensitive actin mutant and exacerbated in cells lacking Ase1, which cross-links antiparallel interpolar microtubules at the spindle midzone both before and after anaphase. These data indicate that both Ase1 and an intact actin cytoskeleton are required for preanaphase spindle stability. Finally, we show that loss of Ase1 activates a checkpoint that requires only the Mad3, Bub1, and Mph1, but not Mad1, Mad2, or Bub3 checkpoint proteins

Weight stigma and physical health: an unconsidered ‘obesity’ cost

This is the author accepted mansuscript. The final version is available from Springer via the DOI in this recordLetter to the Editor: Response to Singh et al. (2018). Is there more to the equation? Weight bias and the costs of obesity. Canadian Journal of Public Health. https://doi.org/10.17269/s41997-018-0146-2. Online first: 26 October 2018Economic and Social Research Council (ESRC

Groundbased near-IR observations of the surface of Venus

We present images of the nightside of Venus taken in the near-infrared windows at 1.0, 1.1, 1.18, 1.28, 1.31, and 2.3 microns with the new infrared camera/spectrometer IRIS on the Anglo-Australian Telescope. These data were taken in spectral-mapping mode. This technique involves scanning the telescope perpendicular to the slit, while collecting spectra at successive slit positions across the planet. We produce data cubes with one spectral and two spatial dimensions. Images can be extracted over any wavelength regions. Each image has square pixels of 0.8 inch resolution. We reduced the scattered light from the sunlit crescent in images extracted from each window by subtracting images taken on either side of the window, where the Venus atmosphere is opaque. Unlike the short wavelength windows, which reveal thermal contrasts that originate primarily from the surface and deep atmosphere, the emission in the 2.3 microns window is produced at much higher altitudes (30-40 km). Emission contrasts seen near 2.3 microns are associated with horizontal variations in the cloud optical depths, and have rotation periods of about six days. We detect large contrasts in infrared emission (20-40 percent) across the disc of Venus in the 1.0-, 1.1-, 1.18-, 1.28-, and 1.31-micron images. Contrasts at these wavelengths may be due to a combination of variations in the optical depths of the overlying sulfuric acid clouds and differences in surface emission. Comparison with the 2.3-micron images show that the patterns seen in the 1.28- and 1.31-micron windows are consistent with cloud optical depth variations alone and require no contribution from the surface. However, images at 1.0, 1.1, and 1.8 microns from July 1991 show a dark feature having a contrast that increases with decreasing wavelength. This behavior is contrary to that expected of cloud absorption. Images taken on three successive days in October show another dark feature that is stationary with respect to the surface. These regions of lower emission correspond closely to the high-altitude surface regions of Beta Regio and Aphrodite Terra. The images can potentially reveal the near-infrared emissiveity of the surface of Venus, thereby complementing Magellan radar reflectivity and ground based radio emissivity measurements. The contrast ratio between highlands and plains is much smaller than would be expected for blackbody radiation from the surface along. Unlike at radio wavelengths, where the atmosphere is essentially transparent, at near-infrared wavelengths the atmosphere emits, absorbs, and scatters radiation, and can modify the observed topographically induced contrasts. The additional radiation from the atmosphere reduces the contrast, and further modification would be expected if terrain at different altitudes has different emissivities. A fit to our data therefore requires, and may constrain, a model of the lowest scale height of the atmosphere

(SNP090) Cleadus Meadows interviewed by Dorothy Noble Smith, transcribed by Sharon G. Marston

Records an interview with Cleadus Meadows, who grew up near Thoroughfare Mountain, in Madison County, Virginia. Describes daily life in the mountains, including raising livestock, the means of growing and preserving food and other aspects of the local economy, such as chestnut harvesting, tan bark and moonshine.https://commons.lib.jmu.edu/snp/1075/thumbnail.jp

Real-world shock: transition shock and its effect on new interpreters of American Sign Language and English

Real-world shock has historically been defined as a form of transition shock experienced during the transition from the academic to the professional world. It is marked by distinct phases and causes both emotional and physical stress. Previous research has found that real-world shock is experienced by new members of a variety of fields, especially training-intensive service professions such as education and medicine. However, even though the profession of American Sign Language/English interpreting is also a training-intensive service profession, there is no research that indicates whether or not new members of the profession experience similar shock to their counterparts in other professions. As such, this thesis shall attempt to determine if real-world shock is experienced by new interpreters of American Sign Language. To do so, a survey was electronically distributed to American Sign Language/English interpreters throughout the United States of America. The resulting data collected was analyzed to determine if new interpreters experience real-world shock and, if so, what the root cause of it is. It was found that interpreters of American Sign Language and English do experience real-world shock upon entering the profession. Furthermore, the shock is a result of the profession rather than from intercultural interactions with the d/Deaf community

Interstate Agreement on Detainers and the Rights It Created

Prior to 1984, the Interstate Agreement on Detainers (lAD) generated very little concern. However, under a theory recently put forth by defense attorneys for accused serial killer Alton Coleman the IAD could provide a mechanism which would interfere with the execution of the death penalty. This article will explore this issue in detail, but before doing so, a discussion of the workings of the IAD is appropriate in order to better appreciate the consequences of this theory. This article will first explore the effects of the detainer process and the events which led to the development of the IAD. Second, it will discuss the application of the IAD\u27s procedural safeguards. Third, the ability of both the prisoners and the states to circumvent the IAD\u27s protection will be analyzed. Finally, this article will examine whether certain prisoners can avoid or delay execution of the death penalty through strict compliance with the IAD