648 research outputs found
Tree regeneration and spatial patterning among midtolerant tree species following gap-based harvesting in a temperate hardwood forest
IntroductionField experiments of gap-based harvest systems in temperate northern hardwood forests have provided inconsistent support for the theory that such regeneration approaches can improve regeneration success among increasingly underrepresented tree species intermediate in shade tolerance. We established a field experiment in Wisconsin, USA testing the long-term response of tree regeneration to group selection harvesting that created small (50 m2–D:H 0.32), medium (200 m2—D:H 0.64) and large (380 m2—D:H 0.88) gaps.Materials and methodsLight levels were modeled to test the assumption of resource heterogeneity within and across these gap sizes. Spatial statistics were used to assess whether the point patterns of saplings of several midtolerant tree species (Betula alleghaniensis, Carya cordiformis, Fraxinus spp.) mapped in harvest gaps provided evidence for partitioning across resource gradients 9 years post-harvest. Finally, we measured occurrence and height of all saplings within the harvest gaps to test the effects of gap size and deer exclusion fencing on the density and total height of shade-tolerant and midtolerant tree regeneration.Results and discussionLight levels progressively increased with gap size, varied by position within gaps, and provided significant evidence of resource heterogeneity. The complex light gradients formed both within and across the three gap sizes may partially explain the statistically significant clustering of Fraxinus spp. saplings in gap centers and north and east into the forested edge of medium and large gaps, C. cordiformis on the west sides of forested transitions surrounding large gaps, and B. alleghaniensis in the centers of large gaps. Densities of tolerant and midtolerant saplings were similar in medium and large gaps after nine growing seasons, though tolerant saplings averaged two meters taller across all three gap sizes. Our results show that gap-based silvicultural systems can create resource gradients that are sufficient to regenerate mixtures of species with high to intermediate tolerance of shade. However, prescriptions beyond harvesting and deer exclusion fencing may be necessary for the recruitment of midtolerant species to canopy positions
Radiative Transfer for Exoplanet Atmospheres
Remote sensing of the atmospheres of distant worlds motivates a firm
understanding of radiative transfer. In this review, we provide a pedagogical
cookbook that describes the principal ingredients needed to perform a radiative
transfer calculation and predict the spectrum of an exoplanet atmosphere,
including solving the radiative transfer equation, calculating opacities (and
chemistry), iterating for radiative equilibrium (or not), and adapting the
output of the calculations to the astronomical observations. A review of the
state of the art is performed, focusing on selected milestone papers.
Outstanding issues, including the need to understand aerosols or clouds and
elucidating the assumptions and caveats behind inversion methods, are
discussed. A checklist is provided to assist referees/reviewers in their
scrutiny of works involving radiative transfer. A table summarizing the
methodology employed by past studies is provided.Comment: 7 pages, no figures, 1 table. Filled in missing information in
references, main text unchange
Pastoral care use among post-9/11 veterans who screen positive for mental health problems.
As a result of their military experience, veterans with mental health problems may have unique motivations for seeking help from clergy. Patterns and correlates of seeking pastoral care were examined using a nationwide representative survey that was conducted among veterans of post-9/11 conflicts (adjusted N = 1,068; 56% response rate). Separate multivariate logistic regression models were used to examine veteran characteristics associated with seeking pastoral care and seeking mental health services. Among post-9/11 veterans with a probable mental disorder (n = 461) – defined as a positive screen for posttraumatic stress disorder, major depressive disorder, or alcohol misuse – 20.2% reported talking to a “pastoral counselor” in the preceding year, 44.7% reported talking to a mental health professional, and 46.6% reported talking to neither. In a multivariate analysis for veterans with a probable mental disorder, seeing a pastoral counselor was associated with an increased likelihood of seeing a mental health professional in the past year (OR: 2.16; 95% CI: [1.28, 3.65]). In a separate bivariate analysis, pastoral counselors were more likely to be seen by veterans who indicated concerns about stigma or distrust of mental health care. These results suggest that pastoral and mental health care services may complement one another and underscore the importance of enhancing understanding and collaboration between these disciplines so as to meet the needs of the veterans they serve
Neurochemical Changes in the Mouse Hippocampus Underlying the Antidepressant Effect of Genetic Deletion of P2X7 Receptors.
Recent investigations have revealed that the genetic deletion of P2X7 receptors (P2rx7) results in an antidepressant phenotype in mice. However, the link between the deficiency of P2rx7 and changes in behavior has not yet been explored. In the present study, we studied the effect of genetic deletion of P2rx7 on neurochemical changes in the hippocampus that might underlie the antidepressant phenotype. P2X7 receptor deficient mice (P2rx7-/-) displayed decreased immobility in the tail suspension test (TST) and an attenuated anhedonia response in the sucrose preference test (SPT) following bacterial endotoxin (LPS) challenge. The attenuated anhedonia was reproduced through systemic treatments with P2rx7 antagonists. The activation of P2rx7 resulted in the concentration-dependent release of [3H]glutamate in P2rx7+/+ but not P2rx7-/- mice, and the NR2B subunit mRNA and protein was upregulated in the hippocampus of P2rx7-/- mice. The brain-derived neurotrophic factor (BDNF) expression was higher in saline but not LPS-treated P2rx7-/- mice; the P2rx7 antagonist Brilliant blue G elevated and the P2rx7 agonist benzoylbenzoyl ATP (BzATP) reduced BDNF level. This effect was dependent on the activation of NMDA and non-NMDA receptors but not on Group I metabotropic glutamate receptors (mGluR1,5). An increased 5-bromo-2-deoxyuridine (BrdU) incorporation was also observed in the dentate gyrus derived from P2rx7-/- mice. Basal level of 5-HT was increased, whereas the 5HIAA/5-HT ratio was lower in the hippocampus of P2rx7-/- mice, which accompanied the increased uptake of [3H]5-HT and an elevated number of [3H]citalopram binding sites. The LPS-induced elevation of 5-HT level was absent in P2rx7-/- mice. In conclusion there are several potential mechanisms for the antidepressant phenotype of P2rx7-/- mice, such as the absence of P2rx7-mediated glutamate release, elevated basal BDNF production, enhanced neurogenesis and increased 5-HT bioavailability in the hippocampus
Contribution of Cystine-Glutamate Antiporters to the Psychotomimetic Effects of Phencyclidine
Altered glutamate signaling contributes to a myriad of neural disorders, including schizophrenia. While synaptic levels are intensely studied, nonvesicular release mechanisms, including cystine–glutamate exchange, maintain high steady-state glutamate levels in the extrasynaptic space. The existence of extrasynaptic receptors, including metabotropic group II glutamate receptors (mGluR), pose nonvesicular release mechanisms as unrecognized targets capable of contributing to pathological glutamate signaling. We tested the hypothesis that activation of cystine–glutamate antiporters using the cysteine prodrug N-acetylcysteine would blunt psychotomimetic effects in the rodent phencyclidine (PCP) model of schizophrenia. First, we demonstrate that PCP elevates extracellular glutamate in the prefrontal cortex, an effect that is blocked by N-acetylcysteine pretreatment. To determine the relevance of the above finding, we assessed social interaction and found that N-acetylcysteine reverses social withdrawal produced by repeated PCP. In a separate paradigm, acute PCP resulted in working memory deficits assessed using a discrete trial t-maze task, and this effect was also reversed by N-acetylcysteine pretreatment. The capacity of N-acetylcysteine to restore working memory was blocked by infusion of the cystine–glutamate antiporter inhibitor (S)-4-carboxyphenylglycine into the prefrontal cortex or systemic administration of the group II mGluR antagonist LY341495 indicating that the effects of N-acetylcysteine requires cystine–glutamate exchange and group II mGluR activation. Finally, protein levels from postmortem tissue obtained from schizophrenic patients revealed significant changes in the level of xCT, the active subunit for cystine–glutamate exchange, in the dorsolateral prefrontal cortex. These data advance cystine–glutamate antiporters as novel targets capable of reversing the psychotomimetic effects of PCP
Mechanisms explaining transitions between tonic and phasic firing in neuronal populations as predicted by a low dimensional firing rate model
Several firing patterns experimentally observed in neural populations have
been successfully correlated to animal behavior. Population bursting, hereby
regarded as a period of high firing rate followed by a period of quiescence, is
typically observed in groups of neurons during behavior. Biophysical
membrane-potential models of single cell bursting involve at least three
equations. Extending such models to study the collective behavior of neural
populations involves thousands of equations and can be very expensive
computationally. For this reason, low dimensional population models that
capture biophysical aspects of networks are needed.
\noindent The present paper uses a firing-rate model to study mechanisms that
trigger and stop transitions between tonic and phasic population firing. These
mechanisms are captured through a two-dimensional system, which can potentially
be extended to include interactions between different areas of the nervous
system with a small number of equations. The typical behavior of midbrain
dopaminergic neurons in the rodent is used as an example to illustrate and
interpret our results.
\noindent The model presented here can be used as a building block to study
interactions between networks of neurons. This theoretical approach may help
contextualize and understand the factors involved in regulating burst firing in
populations and how it may modulate distinct aspects of behavior.Comment: 25 pages (including references and appendices); 12 figures uploaded
as separate file
Impacts on the Hubble Space Telescope Wide Field and Planetary Camera 2: Microanalysis and Recognition of Micrometeoroid Compositions
Postflight surveys of the Wide Field and Planetary Camera 2 (WFPC2) on the Hubble Space Telescope have located hundreds of features on the 2.2 by 0.8 m curved plate, evidence of hypervelocity impact by small particles during 16 years of exposure to space in low Earth orbit (LEO). The radiator has a 100 - 200 micron surface layer of white paint, overlying 4 mm thick Al alloy, which was not fully penetrated by any impact. Over 460 WFPC2 samples were extracted by coring at JSC. About half were sent to NHM in a collaborative program with NASA, ESA and IBC. The structural and compositional heterogeneity at micrometer scale required microanalysis by electron and ion beam microscopes to determine the nature of the impactors (artificial orbital debris, or natural micrometeoroids, MM). Examples of MM impacts are described elsewhere. Here we describe the development of novel electron beam analysis protocols, required to recognize the subtle traces of MM residues
Micrometeoroid Impacts on the Hubble Sace Telescope Wide Field and Planetary Camera 2: Ion Beam Analysis of Subtle Impactor Traces
Recognition of origin for particles responsible for impact damage on spacecraft such as the Hubble Space Telescope (HST) relies upon postflight analysis of returned materials. A unique opportunity arose in 2009 with collection of the Wide Field and Planetary Camera 2 (WFPC2) from HST by shuttle mission STS-125. A preliminary optical survey confirmed that there were hundreds of impact features on the radiator surface. Following extensive discussion between NASA, ESA, NHM and IBC, a collaborative research program was initiated, employing scanning electron microscopy (SEM) and ion beam analysis (IBA) to determine the nature of the impacting grains. Even though some WFPC2 impact features are large, and easily seen without the use of a microscope, impactor remnants may be hard to find
Becoming Physically Active After Bariatric Surgery is Associated With Improved Weight Loss and Health-Related Quality of Life
The purpose of this study was to determine whether pre- to postoperative increases in physical activity (PA) are associated with weight loss and health-related quality of life (HRQoL) following bariatric surgery. Participants were 199 Roux-en-Y gastric bypass (RYGB) surgery patients. The International Physical Activity Questionnaire (IPAQ) was used to categorize participants into three groups according to their preoperative and /1-year postoperative PA level: (i) Inactive/Active (\u3c200-min/week/≥200-min/week), (ii) Active/Active (≥200-min/week/greater ≥200-min/week) and (iii) Inactive/Inactive (\u3c200-min/week/\u3c200-min/week). The Medical Outcomes Study Short Form-36 (SF-36) was used to assess HRQoL. Analyses of covariance were conducted to examine the effects of PA group on weight and HRQoL changes. Inactive/Active participants, compared with Inactive/Inactive individuals, had greater reductions in weight (52.5 ± 15.4 vs. 46.4 ± 12.8 kg) and BMI (18.9 ± 4.6 vs. 16.9 ± 4.2 kg/m2). Weight loss outcomes in the Inactive/Active and Active/Active groups were similar to each other. Inactive/Active and Active/Active participants reported greater improvements than Inactive/Inactive participants on the mental component summary (MCS) score and the general health, vitality and mental health domains (P \u3c 0.01). Although the direction of causation is not clear, these findings suggest that RYGB patients who become active postoperatively achieve weight losses and HRQoL improvements that are greater than those experienced by patients who remain inactive and comparable to those attained by patients who stay active. Future randomized controlled trials should examine whether assisting patients who are inactive preoperatively to increase their PA postoperatively contributes to optimization of weight loss and HRQoL outcomes
Comparison of tolerability and adverse symptoms in oxcarbazepine and carbamazepine in the treatment of trigeminal neuralgia and neuralgiform headaches using the Liverpool Adverse Events Profile (AEP)
Background
Adverse effects of drugs are poorly reported in the literature . The aim of this study was to examine the frequency of the adverse events of antiepileptic drugs (AEDs), in particular carbamazepine (CBZ) and oxcarbazepine (OXC) in patients with neuralgiform pain using the psychometrically tested Liverpool Adverse Events Profile (AEP) and provide clinicians with guidance as to when to change management.
Methods
The study was conducted as a clinical prospective observational exploratory survey of 161 patients with idiopathic trigeminal neuralgia and its variants of whom 79 were on montherapy who attended a specialist clinic in a London teaching hospital over a period of 2 years. At each consultation they completed the AEP questionnaire which provides scores of 19–76 with toxic levels being considered as scores >45.
Results
The most common significant side effects were: tiredness 31.3 %, sleepiness 18.2 %, memory problems 22.7 %, disturbed sleep 14.1 %, difficulty concentrating and unsteadiness 11.6 %. Females reported significantly more side effects than males. Potential toxic dose for females is approximately 1200 mg of OXC and 800 mg of CBZ and1800mg of OXC and 1200 mg of CBZ for males.
Conclusions
CBZ and OXC are associated with cognitive impairment. Pharmacokinetic and pharmacodynamic differences are likely to be the reason for gender differences in reporting side effects. Potentially, females need to be prescribed lower dosages in view of their tendency to reach toxic levels at lower dosages.
Side effects associated with AED could be a major reason for changing drugs or to consider a referral for surgical management
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