Use of Fineness Modulus in Predicting Density of Clean Sand

Relative density is an indicator of compaction level of any granular or cohesionless soil. Sand is preferred for filling work due to its less affinity towards pore moisture content as compared to cohesive soils. It is very difficult to obtain homogeneous sands practically during various earth work operations or other construction practices, resulting in heterogeneous properties in used samples collected from various sources. There are many studies on numerous sand properties but there aren’t many studies relating relative density and fineness modulus of sand. The aim of this research is to carry out an experimental study regarding the relation between the density and fineness modulus of sand and to establish empirical relation between them

Bioluminescent Ratiometric Indicator for Analysis of Water Hardness in Household Water

Water hardness (WH) is a useful parameter for testing household water, such as drinking, cooking, and washing water. Many countries around the world use pipeline water in their houses, but there is a need to monitor the WH because hard water has a negative impact on appliances. Currently, WH is often measured using chemical dye-based WH indicators, and these techniques require expensive equipment, and trained personnel. Therefore, a low-cost and simple measurement method has been desired. Here, we report LOTUS-W, which consists of a luciferase, Nanoluc, a yellow fluorescent protein Venus, and a Ca2+/Mg2+ detection domain of human centrin 3. The binding of Ca2+/Mg2+ to this indicator changes the conformation of human centrin 3, and induces bioluminescence resonance energy transfer (BRET) from Nanoluc to Venus, which changes its emission spectrum about 140%. The dissociation constants of LOTUS-W for Ca2+/Mg2+ are approximately several mM, making it suitable for measuring WH in the household water. With this indicator in combination with a smartphone, we have demonstrated that it is possible to evaluate WH easily and quickly. This novel indicator has the potential to be used for measuring not only household water but also water used in the food industry, etc

Bioluminescent Low-Affinity Ca²⁺ Indicator for ER with Multicolor Calcium Imaging in Single Living Cells

The sarco/endoplasmic reticulum (SR/ER) is the foremost intercellular Ca²⁺ store (at submillimolar concentrations), playing a crucial role in controlling intracellular Ca²⁺ levels. For the investigation of SR/ER Ca²⁺ dynamics in cells, fluorescent protein-based genetically encoded calcium indicators (GECIs) with low Ca²⁺ affinity have been used. Recently, bioluminescent protein-based GECIs with high brightness have been reported to counter the constraints of fluorescence imaging, such as phototoxicity. However, their Ca²⁺ affinity is high and limited for imaging in the cytosol, nucleus, or mitochondria. In this study, we developed a novel cyan color, low-affinity (<i>K</i>_d = 110 μM) intensiometric bioluminescent GECI, which enables monitoring of the Ca²⁺ dynamics in the ER of HeLa cells and the SR of C2C12-derived myotubes. To facilitate the broad concentration range of Ca²⁺ in cellular organelles, we additionally developed an intermediate affinity (<i>K</i>_d = 18 μM), orange color, and bioluminescent GECI, which enables monitoring of Ca²⁺ dynamics in the mitochondria of HeLa cells. With these indicators, in conjunction with an existing high-affinity, green, bioluminescent GECI, we succeeded in multicolor bioluminescent Ca²⁺ imaging in three distinct organelles (nuclei, mitochondria, and ER) simultaneously. The multicolor, live, bioluminescent Ca²⁺ imaging demonstrated here can be used to stably reveal the ER Ca²⁺ homeostasis and cooperative Ca²⁺ regulation among organelles. This will lead to the further understanding of Ca²⁺-related physiological functions and pathophysiological mechanisms

Bioluminescent Low-Affinity Ca²⁺ Indicator for ER with Multicolor Calcium Imaging in Single Living Cells

The sarco/endoplasmic reticulum (SR/ER) is the foremost intercellular Ca²⁺ store (at submillimolar concentrations), playing a crucial role in controlling intracellular Ca²⁺ levels. For the investigation of SR/ER Ca²⁺ dynamics in cells, fluorescent protein-based genetically encoded calcium indicators (GECIs) with low Ca²⁺ affinity have been used. Recently, bioluminescent protein-based GECIs with high brightness have been reported to counter the constraints of fluorescence imaging, such as phototoxicity. However, their Ca²⁺ affinity is high and limited for imaging in the cytosol, nucleus, or mitochondria. In this study, we developed a novel cyan color, low-affinity (<i>K</i>_d = 110 μM) intensiometric bioluminescent GECI, which enables monitoring of the Ca²⁺ dynamics in the ER of HeLa cells and the SR of C2C12-derived myotubes. To facilitate the broad concentration range of Ca²⁺ in cellular organelles, we additionally developed an intermediate affinity (<i>K</i>_d = 18 μM), orange color, and bioluminescent GECI, which enables monitoring of Ca²⁺ dynamics in the mitochondria of HeLa cells. With these indicators, in conjunction with an existing high-affinity, green, bioluminescent GECI, we succeeded in multicolor bioluminescent Ca²⁺ imaging in three distinct organelles (nuclei, mitochondria, and ER) simultaneously. The multicolor, live, bioluminescent Ca²⁺ imaging demonstrated here can be used to stably reveal the ER Ca²⁺ homeostasis and cooperative Ca²⁺ regulation among organelles. This will lead to the further understanding of Ca²⁺-related physiological functions and pathophysiological mechanisms

Bioluminescent Low-Affinity Ca²⁺ Indicator for ER with Multicolor Calcium Imaging in Single Living Cells

The sarco/endoplasmic reticulum (SR/ER) is the foremost intercellular Ca²⁺ store (at submillimolar concentrations), playing a crucial role in controlling intracellular Ca²⁺ levels. For the investigation of SR/ER Ca²⁺ dynamics in cells, fluorescent protein-based genetically encoded calcium indicators (GECIs) with low Ca²⁺ affinity have been used. Recently, bioluminescent protein-based GECIs with high brightness have been reported to counter the constraints of fluorescence imaging, such as phototoxicity. However, their Ca²⁺ affinity is high and limited for imaging in the cytosol, nucleus, or mitochondria. In this study, we developed a novel cyan color, low-affinity (<i>K</i>_d = 110 μM) intensiometric bioluminescent GECI, which enables monitoring of the Ca²⁺ dynamics in the ER of HeLa cells and the SR of C2C12-derived myotubes. To facilitate the broad concentration range of Ca²⁺ in cellular organelles, we additionally developed an intermediate affinity (<i>K</i>_d = 18 μM), orange color, and bioluminescent GECI, which enables monitoring of Ca²⁺ dynamics in the mitochondria of HeLa cells. With these indicators, in conjunction with an existing high-affinity, green, bioluminescent GECI, we succeeded in multicolor bioluminescent Ca²⁺ imaging in three distinct organelles (nuclei, mitochondria, and ER) simultaneously. The multicolor, live, bioluminescent Ca²⁺ imaging demonstrated here can be used to stably reveal the ER Ca²⁺ homeostasis and cooperative Ca²⁺ regulation among organelles. This will lead to the further understanding of Ca²⁺-related physiological functions and pathophysiological mechanisms