The strong environmental dependence of black hole scaling relations

We investigate how the scaling relations between central black hole mass (Mbh) and host galaxy properties (velocity dispersion, bulge stellar mass and bulge luminosity) depend on the large scale environment. For each of a sample of 69 galaxies with dynamical black hole measurements we compile four environmental measures (nearest neighbor distance, fixed aperture number density, total halo mass, and central/satellite). We find that central and satellite galaxies follow distinctly separate scalings in each of the three relations we have examined. The Mbh - sigma relation of central galaxies is significantly steeper (6.38 +/- 0.49) than that of satellite galaxies (4.91 +/- 0.49), but has a similar intercept. This behavior remains even after restricting to a sample of only early type galaxies or after removing the 8 brightest cluster galaxies. The Mbh - sigma relation shows more modest differences when splitting the sample based on the other environmental indicators, suggesting that they are driven by the underlying satellite/central fractions. Separate relations for centrals and satellites are also seen in the power law scaling between black hole mass and bulge stellar mass or bulge luminosity. We suggest that gas rich, low mass galaxies undergo a period of rapid black hole growth in the process of becoming satellites. If central galaxies on the current Mbh - sigma relation are representative progenitors of the satellite population, the observations imply that a sigma = 120 km/s galaxy must nearly triple its central black hole mass. The elevated black hole masses of massive central galaxies are then a natural consequence of the accretion of satellites.Comment: 16 pages, MNRAS, in pres

Exercise and MEF2–HDAC interactions.

Exercise increases the metabolic capacity of skeletal muscle, which improves whole-body energy homeostasis and contributes to the positive health benefits of exercise. This is, in part, mediated by increases in the expression of a number of metabolic enzymes, regulated largely at the level of transcription. At a molecular level, many of these genes are regulated by the class II histone deacetylase (HDAC) family of transcriptional repressors, in particular HDAC5, through their interaction with myocyte enhancer factor 2 transcription factors. HDAC5 kinases, including 5&prime;-AMP-activated protein kinase and protein kinase D, appear to regulate skeletal muscle metabolic gene transcription by inactivating HDAC5 and inducing HDAC5 nuclear export. These mechanisms appear to participate in exercise-induced gene expression and could be important for skeletal muscle adaptations to exercise.<br /

Metallicity beats sSFR: The connection between superluminous supernova host galaxy environments and the importance of metallicity for their production

We analyse 33 Type I superluminous supernovae (SLSNe) taken from ZTF's Bright Transient Survey to investigate the local environments of their host galaxies. We use a spectroscopic sample of galaxies from the SDSS to determine the large-scale environmental density of the host galaxy. Noting that SLSNe are generally found in galaxies with low stellar masses, high star formation rates, and low metallicities, we find that SLSN hosts are also rarely found within high-density environments. Only $3\substack{+9 \\-1}$ per cent of SLSN hosts were found in regions with 2 or more bright galaxies within 2 Mpc. For comparison, we generate a sample of 662 SDSS galaxies matched to the photometric properties of the SLSN hosts. This sample is also rarely found within high-density environments, suggesting that galaxies with properties required for SLSN production favour more isolated environments. Furthermore, we select galaxies within the Illustris-TNG simulation to match SLSN host galaxy properties in colour and stellar mass. We find that the fraction of simulated galaxies in high-density environments quantitatively matches the observed SLSN hosts only if we restrict to simulated galaxies with metallicity $12+\log($O/H$) \leq 8.12$. In contrast, limiting to only the highest sSFR galaxies in the sample leads to an overabundance of SLSN hosts in high-density environments. Thus, our measurement of the environmental density of SLSN host galaxies appears to break the degeneracy between low-metallicity or high-sSFR as the driver for SLSN hosts and provides evidence that the most constraining factor on SLSN production is low-metallicity.Comment: Accepted to MNRAS, 10 pages, 6 figures. Re-uploaded for updating reference

Metabolic profile analysis of zebrafish embryos

A growing goal in the field of metabolism is to determine the impact of genetics on different aspects of mitochondrial function. Understanding these relationships will help to understand the underlying etiology for a range of diseases linked with mitochondrial dysfunction, such as diabetes and obesity. Recent advances in instrumentation, has enabled the monitoring of distinct parameters of mitochondrial function in cell lines or tissue explants. Here we present a method for a rapid and sensitive analysis of mitochondrial function parameters in vivo during zebrafish embryonic development using the Seahorse bioscience XF 24 extracellular flux analyser. This protocol utilizes the Islet Capture microplates where a single embryo is placed in each well, allowing measurement of bioenergetics, including: (i) basal respiration; (ii) basal mitochondrial respiration (iii) mitochondrial respiration due to ATP turnover; (iv) mitochondrial uncoupled respiration or proton leak and (iv) maximum respiration. Using this approach embryonic zebrafish respiration parameters can be compared between wild type and genetically altered embryos (mutant, gene over-expression or gene knockdown) or those manipulated pharmacologically. It is anticipated that dissemination of this protocol will provide researchers with new tools to analyse the genetic basis of metabolic disorders in vivo in this relevant vertebrate animal model

A physiological drop in pH decreases mitochondrial respiration, and HDAC and Akt signaling, in L6 myocytes

Exercise stimulates mitochondrial biogenesis and increases mitochondrial respiratory function and content. However, during high-intensity exercise muscle pH can decrease below pH 6.8 with a concomitant increase in lactate concentration. This drop in muscle pH is associated with reduced exercise-induced mitochondrial biogenesis, while increased lactate may act as a signaling molecule to affect mitochondrial biogenesis. Therefore, in this study we wished to determine the impact of altering pH and lactate concentration in L6 myotubes on genes and proteins known to be involved in mitochondrial biogenesis. We also examined mitochondrial respiration in response to these perturbations. Differentiated L6 myotubes were exposed to normal (pH 7.5)-, low (pH 7.0)-, or high (pH 8.0)-pH media with and without 20 mM sodium l-lactate for 1 and 6 h. Low pH and 20 mM sodium l-lactate resulted in decreased Akt (Ser473) and AMPK (T172) phosphorylation at 1 h compared with controls, while at 6 h the nuclear localization of histone deacetylase 5 (HDAC5) was decreased. When the pH was increased both Akt (Ser473) and AMPK (T172) phosphorylation was increased at 1 h. Overall increased lactate decreased the nuclear content of HDAC5 at 6 h. Exposure to both high- and low-pH media decreased basal mitochondrial respiration, ATP turnover, and maximum mitochondrial respiratory capacity. These data indicate that muscle pH affects several metabolic signaling pathways, including those required for mitochondrial function

CFHT Legacy Ultraviolet Extension (CLUE): witnessing galaxy transformations up to 7 Mpc from rich cluster cores

Using the optical data from the Wide component of the Canada-France-Hawaii Telescope (CFHT) Legacy Survey, and new ultraviolet (UV) data from GALEX, we study the colours and specific star formation rates (SSFRs) of ∼ 100 galaxy clusters at 0.16 < z < 0.36, over areas extending out to radii of r∼ 7 Mpc. We use a multicolour, statistical background subtraction method to study the galaxy population at this radius; thus our results pertain to those galaxies which constitute an excess over the average field density. We find that the average SSFR and its distribution of the star-forming galaxies (with at z∼ 0.2 and at z∼ 0.3) have no measurable dependence on the clustercentric radius and are consistent with the field values. However, the fraction of galaxies with SFR above these thresholds, and the fraction of optically blue galaxies, are lower for the overdense galaxy population in the cluster outskirts compared with the average field value, at all stellar masses and at all radii out to at least 7 Mpc. Most interestingly, the fraction of blue galaxies that are forming stars at a rate below our UV detection limit is much higher in all radial bins around our cluster sample compared with the general field value. This is most noticeable for massive galaxies ; while almost all blue field galaxies of this mass have detectable star formation, this is true for less than 20 per cent of the blue cluster galaxies, even at 7 Mpc from the cluster centre. Our results support a scenario where galaxies are pre-processed in locally overdense regions in a way that reduces their SFR below our UV detection limit, but not to zer

The GEEC2 spectroscopic survey of Galaxy Groups at 0.8<z<10.8<z<1

We present the data release of the Gemini-South GMOS spectroscopy in the fields of 11 galaxy groups at $0.8<z<1$, within the COSMOS field. This forms the basis of the Galaxy Environment Evolution Collaboration 2 (GEEC2) project to study galaxy evolution in haloes with $M\sim 10^{13}M_\odot$ across cosmic time. The final sample includes $162$ spectroscopically--confirmed members with $R50$ per cent complete for galaxies within the virial radius, and with stellar mass $M_{\rm star}>10^{10.3}M_\odot$. Including galaxies with photometric redshifts we have an effective sample size of $\sim 400$ galaxies within the virial radii of these groups. We present group velocity dispersions, dynamical and stellar masses. Combining with the GCLASS sample of more massive clusters at the same redshift we find the total stellar mass is strongly correlated with the dynamical mass, with $\log{M_{200}}=1.20\left(\log{M_{\rm star}}-12\right)+14.07$. This stellar fraction of $~\sim 1$ per cent is lower than predicted by some halo occupation distribution models, though the weak dependence on halo mass is in good agreement. Most groups have an easily identifiable most massive galaxy (MMG) near the centre of the galaxy distribution, and we present the spectroscopic properties and surface brightness fits to these galaxies. The total stellar mass distribution in the groups, excluding the MMG, compares well with an NFW profile with concentration $4$, for galaxies beyond $\sim 0.2R_{200}$. This is more concentrated than the number density distribution, demonstrating that there is some mass segregation.Comment: Accepted for publication in MNRAS. The appendix is omitted due to large figures. The full version will be available from the MNRAS website and from http://quixote.uwaterloo.ca/~mbalogh/papers/GEEC2_data.pdf. Long data tables are available from MNRAS or by contacting the first autho

Reduced glycogen availability is associated with increased AMPKα2 activity, nuclear AMPKα2 protein abundance, and GLUT4 mRNA expression in contracting human skeletal muscle

Glycogen availability can influence glucose transporter 4 (GLUT4) expression in skeletal muscle through unknown mechanisms. The multisubstrate enzyme AMP-activated protein kinase (AMPK) has also been shown to play an important role in the regulation of GLUT4 expression in skeletal muscle. During contraction, AMPK [alpha]2 translocates to the nucleus and the activity of this AMPK isoform is enhanced when skeletal muscle glycogen is low. In this study, we investigated if decreased pre-exercise muscle glycogen levels and increased AMPK [alpha]2 activity reduced the association of AMPK with glycogen and increased AMPK [alpha]2 translocation to the nucleus and GLUT4 mRNA expression following exercise. Seven males performed 60 min of exercise at ~70% [VO.sub.2] peak on 2 occasions: either with normal (control) or low (LG) carbohydrate pre-exercise muscle glycogen content. Muscle samples were obtained by needle biopsy before and after exercise. Low muscle glycogen was associated with elevated AMPK [alpha]2 activity and acetyl-CoA carboxylase [beta] phosphorylation, increased translocation of AMPK [alpha]2 to the nucleus, and increased GLUT4 mRNA. Transfection of primary human myotubes with a constitutively active AMPK adenovirus also stimulated GLUT4 mRNA, providing direct evidence of a role of AMPK in regulating GLUT4 expression. We suggest that increased activation of AMPK [alpha]2 under conditions of low muscle glycogen enhances AMPK [alpha]2 nuclear translocation and increases GLUT4 mRNA expression in response to exercise in human skeletal muscle. <br /

GASP. XII. The variety of physical processes occurring in a single galaxy group in formation

GAs Stripping Phenomena in galaxies with MUSE (GASP) is a program aimed at studying gas removal processes in nearby galaxies in different environments. We present the study of four galaxies that are part of the same group (z= 0.06359) and highlight the multitude of mechanisms affecting the spatially resolved properties of the group members. One galaxy is passive and shows a regular stellar kinematics. The analysis of its star formation history indicates that the quenching process lasted for a few Gyr and that the star formation declined throughout the disk in a similar way, consistent with strangulation. Another galaxy is characterised by a two-component stellar disk with an extended gas disk that formed a few 10^8 yr ago, most likely as a consequence of gas accretion. The third member is a spiral galaxy at the edges of the group, but embedded in a filament. We hypothesise that the compression exerted by the sparse intergalactic medium on the dense circumgalactic gas switches on star formation in a number of clouds surrounding the galaxy ("cosmic web enhancement"). Alternatively, also ram pressure stripping might be effective. Finally, the fourth galaxy is a spiral with a truncated ionised gas disk and an undisturbed stellar kinematics. An analytical model of the galaxy's restoring pressure, and its location and velocity within the cluster, suggest ram pressure is the most likely physical mechanism in action. This is the first optical evidence for stripping in groups.Comment: 19 page, 12 figure