Temperature stressed males are less attractive to female red flour beetles

Male red flour beetles (Tribolium castaneum) are shown to attract females through an aggression pheromone that may be altered due to varying degrees of stress (Wade, 1984). If these females do follow certain pheromone cues, then we would predict that they have a preference when given a choice between stressed and non-stressed males. This was tested by putting two sets of temperature stressed and non-stressed males in an arena with females and noting each interaction through a series of trials. By taking note of each mating, aggression, and simple encounter, we were able to find that females do prefer a non-stressed male. While stress can suppress multiple mechanisms in beetles, this sexual preference study provides insights into the reproductive processes of this pest and other invertebrates

Female Genital Analysis

R code for analyses used for wild-caught female genital morphological variatio

habitat

Habitat matrix for partial mantel tes

FemaleGenital_EMMs

Estimated Marginal Means from MANCOVA for female genital morphological variatio

Male Genitalia Data

Landmarks from geometric morphometrics for analysis of wild-caught male genitali

GenitalMantel

R code to perform partial mantel tests for correlations among female and male genital trait

Female Data

Measurements of female Poecilia mexicana urogenital aperture morpholog

MaleGenitalEMMs

Estimated Marginal Means from MANCOVA for male gonopodium morphological variatio

Male Genital Analysis

R code to perform analyses used for gonopodium shape variatio

FST

Pairwise Fst among P. mexicana population