Visual Scanning of Dynamic Affective Stimuli in Autism Spectrum Disorders

The accurate integration of audio-visual emotion cues is critical for social interactions and requires efficient processing of facial cues. Gaze behavior of typically developing (TD) individuals and individuals with autism spectrum disorders (ASD) was measured via eye-tracking during the perception of dynamic audio-visual emotion (DAVE) stimuli. This study provides information about the regions of the face sampled during an emotion perception task that is relatively more complex than those used in previous studies, providing both bimodal (auditory and visual) and dynamic (biological motion) cues. Results indicated that the ASD group was less accurate at emotion detection and demonstrated less of a visual-affective bias than TD individuals. Both groups displayed similar fixation patterns across regions during the perception of congruent audio-visual stimuli. However, between-group analyses revealed that fixation patterns differed significantly by facial regions during the perception of both congruent and incongruent movies together. In addition, fixation duration to critical regions (i.e., face, core, eyes) was negatively correlated with measures of ASD symptomatology and social impairment. Findings suggest weaknesses in the early integration of audio-visual information, automatic perception of emotion, and efficient detection of affective conflict in individuals with ASD. Implications for future research and social skills intervention programs are discussed

Gaze Fixation during the Perception of Visual and Auditory Affective Cues

The accurate integration of audio-visual emotion cues is critical for social interactions and requires efficient processing of facial cues. Gaze behavior of typically developing young adults was measured via eye-tracking during the perception of dynamic audio-visual emotion (DAVE) stimuli. Participants were able to identify basic emotions (angry, fearful, happy, neutral) and determine the congruence of facial expression and prosody. Perception of incongruent videos resulted in increased reaction times and emotion identification consistent with the facial expression. Participants consistently demonstrated a featural processing approach across all tasks, with a significant preference for the eyes. Evidence of hemispheric lateralization was indicated by preferential fixation to the left (happy, angry) or right eye (fearful). Fixation patterns differed according to the facially expressed emotion, with the pattern that emerged during fearful movies supporting the significance of automatic threat processing. Finally, fixation pattern during the perception of incongruent movies varied according to task instructions

Thermal and Solution Stability of Lysozyme in the Presence of Sucrose, Glucose, and Trehalose

The effect of the sugars sucrose, glucose, and trehalose on the structural and colloidal stability of lysozyme has been investigated using differential scanning calorimetry and quasi-elastic light scattering, respectively. While sugars are known to increase the temperature at which thermal denaturation of protein occurs, it is not clear if, under the same solution conditions, greater colloidal stability is achieved. The measurements were carried out on lysozyme in three different buffer solutions, 0.05 M sodium acetate (pH 4.6), 0.05 M sodium acetate with 5% (w/v) NaCl, and 10 mM sodium phosphate (pH 7.0). The results show that enhancement of structural stability in the presence of sugars is pH, salt concentration, and sugar dependent. Enhancement of colloidal stability in the presence of sugars, while also pH and salt concentration dependent, as expected, only correlates with increases in the structural stability when the solution behavior is not dominated by highly stabilizing electrostatic repulsive interactions

Phase transition in the Ising model on a small-world network with distance-dependent interactions

We study the collective behavior of an Ising system on a small-world network with the interaction $J(r) \propto r^{-\alpha}$, where $r$ represents the Euclidean distance between two nodes. In the case of $\alpha = 0$ corresponding to the uniform interaction, the system is known to possess a phase transition of the mean-field nature, while the system with the short-range interaction $(\alpha\to\infty)$ does not exhibit long-range order at any finite temperature. Monte Carlo simulations are performed at various values of $\alpha$, and the critical value $\alpha_c$ beyond which the long-range order does not emerge is estimated to be zero. Thus concluded is the absence of a phase transition in the system with the algebraically decaying interaction $r^{-\alpha}$ for any nonzero positive value of $\alpha$

Values and M-Services Adoption

The use of mobile services has increased rapidly in recent. Although research has been conducted on which services people use and the benefits they attach to those services, the values associated with the adoption and use of m-services at the individual level is still unclear. This formation systems field various technology adoption models have been proposed and validated in relation to technology adoption within an organisational setting but personal adoption and use of technology is less researched. To help uncover the values behind adoption of m-services we use means-end chains and laddering techniques. The analysis of the interviews shows that mobile services often fulfil such basic needs as self-esteem, achievement, individuality, belonging and well-being. Exploring the realization of values as a theoretical framework offers researchers a way forward in environments characterised by individual technology decisions

Managing the Performance of Asset Acquisition and Operation with Decision Support Tools

Decision support tools (DSTs) are increasingly being used to assist with asset acquisition and management decisions. Whether these tools are “fit for purpose” will have both economic and non-economic implications. Despite this, the on-going governance of DST performance receives only limited attention within both the academic and industry literature. This work addresses that research gap. Within this paper a conceptual process for managing the operational performance of decision support tools is presented. The novelty of the approach is that it aligns with the ISO 5500x:2014 Asset Management Standard, therefore introducing consistency in the governance of DSTs with physical engineered assets. A case study of the UK’s National Grid Electricity Transmission (NGET) is used to inform the approach design. The evaluation demonstrates it to be both logical and useable within the context of NGET and they have expressed an intention to implement the approach within their business. A key finding of the research was that DSTs transcend functions and organisations. This is significant and can lead to a situation whereby performance and criticality are interpreted and measured differently. The introduction of a common approach for managing DST performance works towards improving consistency and creating a shared understanding

CRISPR/Cas-based screening of long non-coding RNAs (lncRNAs) in macrophages with an NF-κB reporter.

The innate immune system protects against infections by initiating an inducible inflammatory response. NF-κB is one of the critical transcription factors controlling this complex response, but some aspects of its regulation remain unclear. For example, although long non-coding RNAs (lncRNAs) have been shown to critically regulate gene expression, only a fraction of these have been functionally characterized, and the extent to which lncRNAs control NF-κB expression is unknown. Here, we describe the generation of a GFP-based NF-κB reporter system in immortalized murine bone marrow-derived macrophages (iBMDM). Activation of this reporter, using Toll-like receptor ligands, resulted in GFP expression, which could be monitored by flow cytometry. We also established a CRISPR/Cas9 gene deletion system in this NF-κB reporter line, enabling us to screen for genes that regulate NF-κB signaling. Our deletion-based approach identified two long intergenic non-coding(linc)RNAs, lincRNA-Cox2 and lincRNA-AK170409, that control NF-κB signaling. We demonstrate a potential novel role for lincRNA-Cox2 in promoting IκBα degradation in the cytoplasm. For lincRNA-AK170409, we provide evidence that this nuclearly-localized lincRNA regulates a number of inflammation-related genes. In conclusion, we have established an NF-κB-GFP iBMDM reporter cell line and a line that stably expresses Cas9. Our approach enabled the identification of lincRNA-Cox2 and lincRNA-AK170409 as NF-κB regulators, and this tool will be useful for identifying additional genes involved in regulating this transcription factor critical for immune function

The Risk of Venous Thromboembolism (VTE) in Men with Benign Prostatic Hyperplasia Treated with 5-Alpha Reductase Inhibitors (5ARIs)

Background: Many men receive 5-alpha reductase inhibitors (5ARIs) for ongoing treatment of benign prostatic hyperplasia (BPH). The increased risk of cardiovascular complications with 5ARIs has been documented in BPH studies and the occurrence of cerebral venous thrombosis, presumably due to increased estrogen level following 5ARI use, was described in multiple case reports. The objective of this study was to determine if 5ARIs with or without alpha blockers (AB) were associated with an increased risk of venous thromboembolism (VTE) in males with BPH. Methods: We conducted a nested case-control study among a population of men ages 40-79 who received at least one 5ARI or AB prescription for treatment of BPH between 1995 and 2015 in the UK-based Clinical Practice Research Datalink GOLD. Cases of incident VTE (pulmonary embolism [PE] or deep venous thrombosis [DVT]) and matched controls were identified from this population. We used descriptive analyses and conditional logistic regression to evaluate the risk of VTE in users of 5ARIs compared to users of ABs. Results: For 5ARI only users, the adjusted odds ratios (aORs), (95% CI) for VTE were 1.51 (0.98-2.32) in current 5ARI users and 1.23 (0.70-2.17) in recent/distant past, compared to AB only users. However, the aOR (95% CI) in men who had 50 or more current 5ARI prescriptions compared to users of ABs only was higher: 2.29 (1.14-4.63). For 5ARI with AB use, the aORs, (95% CI) for VTE were 1.16 (0.64-2.10) in current 5ARI+AB users and 1.93 (0.71-5.25) in recent/distant past, compared to AB only users. The aOR (95% CI) in men who had 50 or more current 5ARI+AB prescriptions compared to users of ABs only was 1.65 (0.64-4.26). Conclusion: Current use of 5ARI, particularly long-term use, is associated with an increased risk of incident idiopathic VTE compared to patients treated with AB use only

Combating Decision Support Tool Performance Decay

Businesses are dynamic environments. If decision support tools do not evolve in line with the organisational requirements they risk suffering from performance decay. This may present as a decline in their use, or a reduction in the value they offer. In collaborationwith National Grid we address this challenge. Our research proposal is that decision support tools should be evaluated for the risk of performance decay. The results of this risk assessment should be used to develop management strategies. Where sustained performance of the existingdecision support tool is the preferred solution, a quality management system approach incorporating continuous improvement should be taken

Juvenile and young adult firearm use in South Carolina : an update and reanalysis of data

This project is a follow-up to and an expansion of, an earlier report, Juvenile and Young Adult Firearm Use in South Carolina. There were two sets of objectives for the original project. The first involved determining the extent to which firearms are involved in violent crimes committed by children and young adults, identifying key characteristics of those arrested for committing these crimes, identifying who was being victimized by these crimes, and discovering under what circumstances these crimes were being committed. The second set of objectives involved learning more about what motivates children to possess firearms in the first place, how they manage to acquire firearms, and what factors are associated with the decision to use or possess firearms. The purpose of this report is to expand upon the first set of objectives and to reanalyze the findings of the second set, with the goal of achieving a higher degree of confidence in the self-reported data