A study of the reactions of third grade pupils to the various types of children's literature that were available during three specific periods: 1900-1920; 1920-1940; 1940-1953

Thesis (Ed.M.)--Boston Universit

Prediction of success in first grade achievement from readiness and intelligence tests

Thesis (Ed.M.)--Boston Universit

Trends in Cannabis-Related and Opioid-Related Hospitalization Rates in Maine, 2010-2020

Introduction: Medical marijuana use became legal in the state of Maine in 1999, and recreational adult-use marijuana became legal in 2016. However, the rates of hospitalization related to marijuana (also known as cannabis) use have not been studied. We estimated trends in cannabis-related hospitalization rates in Maine based on rurality and descriptively compared these trends to opioid-related hospitalization rates during the past decade. Methods: We obtained de-identified data of inpatient encounters for Maine residents between 2010 and 2020 from MaineHealth Data Organization. We then calculated age-standardized rates of cannabis-related and opioid-related hospitalizations overall and by rural-urban residence using International Classification of Diseases clinical modification (ICD-CM) diagnosis codes. We used Poisson regression models to assess linear trends over time, stratified by ICD-9-CM versus ICD-10-CM timeframes. Results: Cannabis-related inpatient encounters accounted for 2.3% of all inpatient encounters among Maine residents between 2010 and 2020 and increased 0.3% per quarter between 2016 and 2020. Rates for urban residents were higher than rates for rural residents. Opioid-related inpatient encounters among Maine residents accounted for 3.4% of all inpatient encounters, and rates increased 0.6% per quarter between 2016 and 2020. Rates were higher among urban versus rural residents. Discussion: We found increasing rates of cannabis-related and opioid-related inpatient encounters in Maine spanning a period that included when recreational adult-use marijuana was legalized, but largely preceded its retail sales in Maine (October 2020). Conclusions: These trends and other public health impacts of increased access to marijuana use in Maine should continue to be monitored, especially after recreational adult-use marijuana is fully implemented

Quasars from the 7C Survey - I:sample selection and radio maps

We describe the selection of candidate radio-loud quasars obtained by cross-matching radio source positions from the low-frequency (151 MHz) 7C survey with optical positions from five pairs of EO POSS-I plates scanned with the Cambridge Automatic Plate-measuring Machine (APM). The sky region studied is centred at RA 10 h 28 m, Dec +41 and covers 0.057 sr. We present VLA observations of the quasar candidates, and tabulate various properties derived from the radio maps. We discuss the selection criteria of the resulting `7CQ' sample of radio-loud quasars. The 70 confirmed quasars, and some fraction of the 36 unconfirmed candidates, constitute a filtered sample with the following selection criteria: 151-MHz flux density S151 > 100 mJy; POSS-I E-plate magnitude E approx R < 20; and POSS-I colour (O - E) < 1.8; the effective area of the survey drops significantly below S151 approx 200 mJy. We argue that the colour criterion excludes few if any quasars, but note, on the basis of recent work by Willott et al. (1998b), that the E magnitude limit probably excludes more than 50 per cent of the radio-loud quasars.Comment: Latex, 30 pages. Accepted by MNRA

Metabolic network analysis reveals microbial community interactions in anammox granules.

Microbial communities mediating anaerobic ammonium oxidation (anammox) represent one of the most energy-efficient environmental biotechnologies for nitrogen removal from wastewater. However, little is known about the functional role heterotrophic bacteria play in anammox granules. Here, we use genome-centric metagenomics to recover 17 draft genomes of anammox and heterotrophic bacteria from a laboratory-scale anammox bioreactor. We combine metabolic network reconstruction with metatranscriptomics to examine the gene expression of anammox and heterotrophic bacteria and to identify their potential interactions. We find that Chlorobi-affiliated bacteria may be highly active protein degraders, catabolizing extracellular peptides while recycling nitrate to nitrite. Other heterotrophs may also contribute to scavenging of detritus and peptides produced by anammox bacteria, and potentially use alternative electron donors, such as H2, acetate and formate. Our findings improve the understanding of metabolic activities and interactions between anammox and heterotrophic bacteria and offer the first transcriptional insights on ecosystem function in anammox granules

De novo synthesis and functional analysis of the phosphatase-encoding gene acI-B of uncultured Actinobacteria from Lake Stechlin (NE Germany)

The National Center for Biotechnology Information [http://www.ncbi.nlm.nih.gov/guide/taxonomy/] database enlists more than 15,500 bacterial species. But this also includes a plethora of uncultured bacterial representations. Owing to their metabolism, they directly influence biogeochemical cycles, which underscores the the important status of bacteria on our planet. To study the function of a gene from an uncultured bacterium, we have undertaken a de novo gene synthesis approach. Actinobacteria of the acI-B subcluster are important but yet uncultured members of the bacterioplankton in temperate lakes of the northern hemisphere such as oligotrophic Lake Stechlin (NE Germany). This lake is relatively poor in phosphate (P) and harbors on average ~1.3 x 106 bacterial cells/ml, whereby Actinobacteria of the ac-I lineage can contribute to almost half of the entire bacterial community depending on seasonal variability. Single cell genome analysis of Actinobacterium SCGC AB141-P03, a member of the acI-B tribe in Lake Stechlin has revealed several phosphate-metabolizing genes. The genome of acI-B Actinobacteria indicates potential to degrade polyphosphate compound. To test for this genetic potential, we targeted the exoP-annotated gene potentially encoding polyphosphatase and synthesized it artificially to examine its biochemical role. Heterologous overexpression of the gene in Escherichia coli and protein purification revealed phosphatase activity. Comparative genome analysis suggested that homologs of this gene should be also present in other Actinobacteria of the acI lineages. This strategic retention of specialized genes in their genome provides a metabolic advantage over other members of the aquatic food web in a P-limited ecosystem. [Int Microbiol 2016; 19(1):39-47]Keywords: acI-B in Actinobacteria &middot; phosphatases &middot; single cell genomics &middot; phosphate limitation &middot; Lake Stechlin, NE German