56 research outputs found

    Videotaping Experiments in an Analytical Chemistry Laboratory Course at Pace University

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    Instructional videos for laboratory experiments performed in an analytical chemistry course were developed to show undergraduate students enrolled in the course how to conduct experiments. Students watched the videos before coming to the laboratory class. The effectiveness of using these videos was evaluated via a postlaboratory survey. The overall response to these videos was positive, with students reporting that the videos helped them to prepare beforehand and to understand the concepts covered in the experiment. The shortened discussion time at the beginning of class resulted in more laboratory time for the students to focus on performing the experiment and for the instructors to supervise, answer questions, make corrections to laboratory techniques, and ensure that the experiment is conducted in a safe manner

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    https://digitalcommons.library.umaine.edu/mmb-vp-copyright/8175/thumbnail.jp

    Local loperamide injection reduces mechanosensitivity of rat cutaneous, nociceptive C-fibers.

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    Loperamide reverses signs of mechanical hypersensitivity in an animal model of neuropathic pain suggesting that peripheral opioid receptors may be suitable targets for the treatment of neuropathic pain. Since little is known about loperamide effects on the responsiveness of primary afferent nerve fibers, in vivo electrophysiological recordings from unmyelinated afferents innervating the glabrous skin of the hind paw were performed in rats with an L5 spinal nerve lesion or sham surgery. Mechanical threshold and responsiveness to suprathreshold stimulation were tested before and after loperamide (1.25, 2.5 and 5 µg in 10 µl) or vehicle injection into the cutaneous receptive field. Loperamide dose-dependently decreased mechanosensitivity in unmyelinated afferents of nerve-injured and sham animals, and this effect was not blocked by naloxone pretreatment. We then investigated loperamide effects on nerve conduction by recording compound action potentials in vitro during incubation of the sciatic nerve with increasing loperamide concentrations. Loperamide dose-dependently decreased compound action potentials of myelinated and unmyelinated fibers (ED50 = 8 and 4 µg/10 µl, respectively). This blockade was not prevented by pre-incubation with naloxone. These results suggest that loperamide reversal of behavioral signs of neuropathic pain may be mediated, at least in part, by mechanisms independent of opioid receptors, most probably by local anesthetic actions

    Naloxone does not block loperamide effects on mechanosensitivity of C-fibers.

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    <p>A) Naloxone pretreatment does not prevent the loperamide (5 µg/10 µl) – induced increase in mechanical thresholds of C-fibers. Naloxone was used either in a small (4 µg in 10 µl, n = 3) or a high dose (80 µg in 20 µl, n = 10). Medians and 25th percentile data are shown. 75th percentiles were identical to median value. (**p<0.01, Wilcoxon matched pairs, n = 13). B) Loperamide –induced decrease in response to suprathreshold mechanical stimulation, is not prevented by naloxone pretreatment. Loperamide and naloxone doses are identical to those used in A. Medians, 25th percentile and 75th percentiles are shown. (**p<0.01, Wilcoxon matched pairs).</p

    The conduction blockade by loperamide was dose-dependent.

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    <p>A) Dose response curves for the A- and C – CAPs were plotted, and regression analyses were performed for both to estimate the ED<sub>50</sub>. For the C-CAP (red symbols), the regression line and the dose response data completely overlap. The ED<sub>50</sub> for C-CAP (4.0 µg/10 µl) is lower than the ED<sub>50</sub> for A-CAP (8.1 µg/10µl), but the 95% confidence intervals overlap slightly. B) The average A-CAP during loperamide incubation was significantly larger than the average C-CAP (inset, paired t-test, p<0.05, n = 10), suggesting that A fibers are less susceptible to loperamide effects.</p
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