Benefit Transfer in the Field: Measuring the Benefits of Heterogeneous Wetlands using Contingent Valuation and Ecological Field Appraisals

Wetlands have functional values that may extend beyond traditional real estate values. This paper uses contingent valuation and ecological field assessments to place heterogeneous values on heterogeneous wetlands. Wetland functions evaluated are water quality, habitat, recreation, storing floodwaters and erosion abatement. The model used incorporates the public value of wetland functions and adds that value to the common local appraisal cost. We use a “percentage willingness-to-pay” value elicitation question in which respondents are asked about the percentage amount that the state government should pay over and above market value to purchase and preserve a wetland function. These values are then mapped into an ecological matrix to value the wetland as a whole. We show how these values can be applied in the field. Key Words: wetlands, appraisal, evaluation, mitigation, contingent valuation methodLength:

Scattering from a Domain Wall in a Spontaneously Broken Gauge Theory

We study the interaction of particles with a domain wall at a symmetry-breaking phase transition by perturbing about the domain wall solution. We find the particulate excitations appropriate near the domain wall and relate them to the particles present far from the wall in the uniform broken and unbroken phases. For a quartic Higgs potential we find analytic solutions to the equations of motion and derive reflection and transmission coefficients. We discover several bound states for particles near the wall. Finally, we apply our results to the electroweak phase transition in the standard model.Comment: 48 pages, 10 figures, LaTeX / epsf, revised to include references to earlier related wor

Expanding P450 catalytic reaction space through evolution and engineering

Advances in protein and metabolic engineering have led to wider use of enzymes to synthesize important molecules. However, many desirable transformations are not catalyzed by any known enzyme, driving interest in understanding how new enzymes can be created. The cytochrome P450 enzyme family, whose members participate in xenobiotic metabolism and natural products biosynthesis, catalyzes an impressive range of difficult chemical reactions that continues to grow as new enzymes are characterized. Recent work has revealed that P450-derived enzymes can also catalyze useful reactions previously accessible only to synthetic chemistry. The evolution and engineering of these enzymes provides an excellent case study for how to genetically encode new chemistry and expand biology's reaction space

The NASA Cubesat Missions Flying on Artemis-1

In 2021, the Space Launch Services (SLS) Artemis-1 mission will carry thirteen 6U CubeSats into deep space. Three of those payloads are NASA missions performing a variety of unique deep-space science experiments. The three NASA CubeSat missions are BioSentinel, Lunar Flashlight and NEAScout. The BioSentinel mission will measure deep-space radiation effects on DNA using yeast organisms. It is a six-month mission in a heliocentric orbit. BioSentinel was designed, built, tested and operated out of NASA Ames Research Center. Lunar Flashlight’s mission is to look for surface water ice in the permanently shadowed regions near the south pole of the Moon and test out new small spacecraft technologies. The spacecraft was developed at the Jet Propulsion Laboratory as a technology demonstration mission with support from the Marshall Space Flight Center, the Goddard Space Flight Center and Georgia Institute of Technology. NEA Scout’s mission is twofold: to demonstrate solar sail deployment and spacecraft navigation using the sail to detect, track, fly by, and characterize a near earth asteroid. NEA Scout was developed by NASA’s Marshall Space Flight Center in partnership with the Jet Propulsion Laboratory, and support from the Goddard Space Flight Center, Lyndon B. Johnson Space Center, and Langley Research Center

Structural Adaptability Facilitates Histidine Heme Ligation in a Cytochrome P450

Almost all known members of the cytochrome P450 (CYP) superfamily conserve a key cysteine residue that coordinates the heme iron. Although mutation of this residue abolishes monooxygenase activity, recent work has shown that mutation to either serine or histidine unlocks non-natural carbene- and nitrene-transfer activities. Here we present the first crystal structure of a histidine-ligated P450. The T213A/C317H variant of the thermostable CYP119 from Sulfolobus acidocaldarius maintains heme iron coordination through the introduced ligand, an interaction that is accompanied by large changes in the overall protein structure. We also find that the axial cysteine C317 may be substituted with any other amino acid without abrogating folding and heme cofactor incorporation. Several of the axial mutants display unusual spectral features, suggesting that they have active sites with unique steric and electronic properties. These novel, highly stable enzyme active sites will be fruitful starting points for investigations of non-natural P450 catalysis and mechanisms

Rotavirus NSP4 is secreted from infected cells as an oligomeric lipoprotein and binds to glycosaminoglycans on the surface of non-infected cells

<p>Abstract</p> <p>Background</p> <p>Nonstructural glycoprotein 4 (NSP4) encoded by rotavirus is the only viral protein currently believed to function as an enterotoxin. NSP4 is synthesized as an intracellular transmembrane glycoprotein and as such is essential for virus assembly. Infection of polarized Caco-2 cells with rotavirus also results in the secretion of glycosylated NSP4 apparently in a soluble form despite retention of its transmembrane domain. We have examined the structure, solubility and cell-binding properties of this secreted form of NSP4 to further understand the biochemical basis for its enterotoxic function. We show here that NSP4 is secreted as discrete detergent-sensitive oligomers in a complex with phospholipids and demonstrate that this secreted form of NSP4 can bind to glycosaminoglycans present on the surface of a range of different cell types.</p> <p>Methods</p> <p>NSP4 was purified from the medium of infected cells after ultracentrifugation and ultrafiltration by successive lectin-affinity and ion exchange chromatography. Oligomerisation of NSP4 was examined by density gradient centrifugation and chemical crosslinking and the lipid content was assessed by analytical thin layer chromatography and flame ionization detection. Binding of NSP4 to various cell lines was measured using a flow cytometric-based assay.</p> <p>Results</p> <p>Secreted NSP4 formed oligomers that contained phospholipid but dissociated to a dimeric species in the presence of non-ionic detergent. The purified glycoprotein binds to the surface of various non-infected cells of distinct lineage. Binding of NSP4 to HT-29, a cell line of intestinal origin, is saturable and independent of divalent cations. Complementary biochemical approaches reveal that NSP4 binds to sulfated glycosaminoglycans on the plasma membrane.</p> <p>Conclusion</p> <p>Our study is the first to analyze an authentic (i.e. non-recombinant) form of NSP4 that is secreted from virus-infected cells. Despite retention of the transmembrane domain, secreted NSP4 remains soluble in an aqueous environment as an oligomeric lipoprotein that can bind to various cell types via an interaction with glycosaminoglycans. This broad cellular tropism exhibited by NSP4 may have implications for the pathophysiology of rotavirus disease.</p

Non-natural Olefin Cyclopropanation Catalyzed by Diverse Cytochrome P450s and Other Hemoproteins

Recent work has shown that engineered variants of cytochrome P450_(BM3) (CYP102A1) efficiently catalyze non-natural reactions, including carbene and nitrene transfer reactions. Given the broad substrate range of natural P450 enzymes, we set out to explore if this diversity could be leveraged to generate a broad panel of new catalysts for olefin cyclopropanation (i.e., carbene transfer). Here, we took a step towards this goal by characterizing the carbene transfer activities of four new wild-type P450s that have different native substrates. All four were active and exhibited a range of product selectivities in the model reaction: cyclopropanation of styrene by using ethyl diazoacetate (EDA). Previous work on P450_(BM3) demonstrated that mutation of the axial coordinating cysteine, universally conserved among P450 enzymes, to a serine residue, increased activity for this non-natural reaction. The equivalent mutation in the selected P450s was found to activate carbene transfer chemistry both in vitro and in vivo. Furthermore, serum albumins complexed with hemin were also found to be efficient in vitro cyclopropanation catalysts

Millimeter Wave Localization: Slow Light and Enhanced Absorption

We exploit millimeter wave technology to measure the reflection and transmission response of random dielectric media. Our samples are easily constructed from random stacks of identical, sub-wavelength quartz and Teflon wafers. The measurement allows us to observe the characteristic transmission resonances associated with localization. We show that these resonances give rise to enhanced attenuation even though the attenuation of homogeneous quartz and Teflon is quite low. We provide experimental evidence of disorder-induced slow light and superluminal group velocities, which, in contrast to photonic crystals, are not associated with any periodicity in the system. Furthermore, we observe localization even though the sample is only about four times the localization length, interpreting our data in terms of an effective cavity model. An algorithm for the retrieval of the internal parameters of random samples (localization length and average absorption rate) from the external measurements of the reflection and transmission coefficients is presented and applied to a particular random sample. The retrieved value of the absorption is in agreement with the directly measured value within the accuracy of the experiment.Comment: revised and expande