Exploring Cost-Effective, High Performance Residential Retrofits for Affordable Housing in the Hot Humid Climate

In 2009, a Department of Energy Building America team led by the Florida Solar Energy Center began working with partners to find cost-effective paths for improving the energy performance of existing homes in the hot humid climate. A test-in energy audit and energy use modeling of the partner’s proposed renovation package was performed for 41 affordable and middle income foreclosed homes in Florida and Alabama. HERS1 Indices ranged from 92 to 184 with modeled energy savings ranging from 3% to 50% (average of 26%). Analyses and recommendations were discussed with partners to encourage more efficient retrofits, highlight health and safety issues, and gather feedback on incremental cost of high performance measures. Ten completed renovations have modeled energy savings ranging from 9% to 48% (average 31%.) This paper presents the project’s process including our findings thus far and highlights of the first home to meet the target HERS Index of 70.Florida Solar Energy Cente

Research Results from A Few Alternate Methods of Interior Duct Systems in Factory Built Housing Located In the Hot Humid Climate

The U.S. Department of Energy’s (DOE) Building America1 Industrialized Housing Partnership (BAIHP) has collaborated with two of its industry partners to work on a portion of the project that relates to the construction and evaluation of prototype interior duct systems. In 2006, work began on a duct system design that would locate the entire length of duct work within the air and thermal barriers of the envelope. One of these designs incorporated a high-side supply register that connects to the conventional floor duct. The other design utilized a single soffit located within the conditioned space at the marriage line. The Florida Solar Energy Center’s (FSEC) Manufactured Housing Lab (MHLab) was retrofitted with an interior soffit duct. The duct system was added on so that either the attic duct system or the new interior duct system would be able to supply air to the conditioned space using the same mechanical equipment. The initial results of this work show approximately a 10% to 20% heating/cooling savings when compared to conventional attic duct work construction techniques and nearly 7% savings when compared to a conventional in-floor system

Next Step Toward Widespread Residential Deep Energy Retrofits

The complexity of deep energy retrofits warrants additional training to successfully manage multiple improvements that will change whole house air, heat, and moisture flow dynamics. The home performance contracting industry has responded to these challenges by aggregating skilled labor for assessment of and implementation under one umbrella. Two emerging business models are profiled that seek to resolve many of the challenges, weaknesses, opportunities, and threats described for the conventional business models

Metabolic rate and rates of protein turnover in food-deprived cuttlefish, Sepia officinalis (Linnaeus 1758)

To determine the metabolic response to food deprivation, cuttlefish (Sepia officinalis) juveniles were either fed, fasted (3 to 5 days food deprivation), or starved (12 days food deprivation). Fasting resulted in a decrease in triglyceride levels in the digestive gland, and after 12 days, these lipid reserves were essentially depleted. Oxygen consumption was decreased to 53% and NH4 excretion to 36% of the fed group following 3-5 days of food deprivation. Oxygen consumption remained low in the starved group, but NH4 excretion returned to the level recorded for fed animals during starvation. The fractional rate of protein synthesis of fasting animals decreased to 25% in both mantle and gill compared with fed animals and remained low in the mantle with the onset of starvation. In gill, however, protein synthesis rate increased to a level that was 45% of the fed group during starvation. In mantle, starvation led to an increase in cathepsin A-, B-, H-, and L-like enzyme activity and a 2.3-fold increase in polyubiquitin mRNA that suggested an increase in ubiquitin-proteasome activity. In gill, there was a transient increase in the polyubiquitin transcript levels in the transition from fed through fasted to the starved state and cathepsin A-, B-, H-, and L-like activity was lower in starved compared with fed animals. The response in gill appears more complex, as they better maintain rates of protein synthesis and show no evidence of enhanced protein breakdown through recognized catabolic processes

Optimizing Manufactured Housing Energy Use

In partnership with the Florida Solar Energy Center (FSEC), two manufactured homes were located on North Carolina A&T State University's campus in Greensboro, NC and used in a side-by-side energy consumption comparison. One of the homes was built to the basic HUD code standard and the other was constructed with features expected to produce a home that was 50% more energy efficient. FSEC and NCATSU began monitoring energy performance in both homes. In addition, the performance of each unit was evaluated using a DOE2 based computer energy analysis program developed by FSEC. A comparison of the performance of the units shows a measured energy savings in the more energy efficient unit of 52% for the Heating, cooling, and DHW energy use. This compares well with the energy savings predicted by the FSEC Energy Gauge program of 57%, even when accounting for the warmer than usual winter experienced during the testing period

Clinical and biochemical function of polymorphic NR0B1 GGAA-microsatellites in Ewing sarcoma: a report from the Children\u27s Oncology Group.

BACKGROUND: The genetics involved in Ewing sarcoma susceptibility and prognosis are poorly understood. EWS/FLI and related EWS/ETS chimeras upregulate numerous gene targets via promoter-based GGAA-microsatellite response elements. These microsatellites are highly polymorphic in humans, and preliminary evidence suggests EWS/FLI-mediated gene expression is highly dependent on the number of GGAA motifs within the microsatellite. OBJECTIVES: Here we sought to examine the polymorphic spectrum of a GGAA-microsatellite within the NR0B1 promoter (a critical EWS/FLI target) in primary Ewing sarcoma tumors, and characterize how this polymorphism influences gene expression and clinical outcomes. RESULTS: A complex, bimodal pattern of EWS/FLI-mediated gene expression was observed across a wide range of GGAA motifs, with maximal expression observed in constructs containing 20-26 GGAA motifs. Relative to white European and African controls, the NR0B1 GGAA-microsatellite in tumor cells demonstrated a strong bias for haplotypes containing 21-25 GGAA motifs suggesting a relationship between microsatellite function and disease susceptibility. This selection bias was not a product of microsatellite instability in tumor samples, nor was there a correlation between NR0B1 GGAA-microsatellite polymorphisms and survival outcomes. CONCLUSIONS: These data suggest that GGAA-microsatellite polymorphisms observed in human populations modulate EWS/FLI-mediated gene expression and may influence disease susceptibility in Ewing sarcoma

Age, Tumor Characteristics, and Treatment Regimen as Event Predictors in Ewing: A Children's Oncology Group Report

. Purpose. To associate baseline patient characteristics and relapse across consecutive COG studies. Methods. We analyzed risk factors for LESFT patients in three randomized COG trials. We evaluated age at enrollment, primary site, gender, tumor size, and treatment (as randomized). We estimated event-free survival (EFS, Kaplan-Meier) and compared risk across groups (log-rank test). Characteristics were assessed by proportional hazards regression with the characteristic of interest as the only component. Confidence intervals (CI) for RR were derived. Factors related to outcome at level 0.05 were included in a multivariate regression model. Results. Between 12/1988 and 8/2005, 1444 patients were enrolled and data current to 2001, 2004, or 2008 were used. Patients were with a median age of 12 years (0-45), 55% male and 88% Caucasian. The 5-year EFS was 68.3% ± 1.3%. In univariate analysis age, treatment, and tumor location were identified for inclusion in the multivariate model, and all remained significant (p < 0.01). Since tumor size was not collected in the last study, the other two were reanalyzed. This model identified age, treatment, tumor location, and tumor size as significant predictors. Conclusion. Age > 18 years, pelvic tumor, size > 8 cms, and chemotherapy without ifosfamide/etoposide significantly predict worse outcome. AEWS0031 is NCT00006734, INT0091 and INT0054 designed before 1993 (unregistered)