47 research outputs found
OpTiDDM (Optical Tweezers integrating Differential Dynamic Microscopy) maps the spatiotemporal propagation of nonlinear stresses in polymer blends and composites
How local stresses propagate through polymeric fluids, and, more generally,
how macromolecular dynamics give rise to viscoelasticity are open questions
vital to wide-ranging scientific and industrial fields. Here, to unambiguously
connect polymer dynamics to force response, and map stress propagation in
macromolecular materials, we present a powerful approach-Optical Tweezers
integrating Differential Dynamic Microscopy (OpTiDMM)-that simultaneously
imposes local strains, measures resistive forces, and analyzes the motion of
the surrounding polymers. Our measurements with blends of ring and linear
polymers (DNA) and their composites with stiff polymers (microtubules) uncover
a surprising resonant response, in which affine alignment, superdiffusivity,
and elastic memory are maximized when the strain rate is comparable to the
entanglement rate. Microtubules suppress this resonance, while substantially
increasing elastic force and memory, due to varying degrees to which the
polymers buildup, stretch and flow along the strain path, and configurationally
dissipate stress. More broadly, the rich multi-scale coupling of mechanics and
dynamics afforded by OpTiDDM, empowers its interdisciplinary use to elucidate
non-trivial phenomena that sculpt stress propagation dynamics-critical to
commercial applications and cell mechanics alike.Comment: 32 pages, 10 figure
The effect of curvature and topology on membrane hydrodynamics
We study the mobility of extended objects (rods) on a spherical liquid-liquid
interface to show how this quantity is modified in a striking manner by both
the curvature and the topology of the interface. We present theoretical
calculations and experimental measurements of the interfacial fluid velocity
field around a moving rod bound to the crowded interface of a water-in-oil
droplet. By using different droplet sizes, membrane viscosities, and rod
lengths, we show that the viscosity mismatch between the interior and exterior
fluids leads to a suppression of the fluid flow on small droplets that cannot
be captured by the flat interface predictions.Comment: 4 pages, 3 figure
Studying the dynamics of colloidal particles with digital holographic microscopy and electromagnetic scattering solutions
Digital holographic microscopy (DHM) can measure the 3D positions as well as the scattering properties of colloidal particles in a single 2D image. We describe DHM and our analysis of recorded holograms with exact scattering solutions, which permit the measurement of 3D particle positions with ∼10 nm precision and millisecond time resolution, and discuss studies of the Brownian dynamics of clusters of spheres with DHM
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Noninvasive measurements of gas exchange in a three-dimensional fluidized bed by hyperpolarized Xe-129 NMR
Force Feedback Controls Motor Activity and Mechanical Properties of Self-Assembling Branched Actin Networks
Branched actin networks–created by the Arp2/3 complex, capping protein, and a nucleation promoting factor– generate and transmit forces required for many cellular processes, but their response to force is poorly understood. To address this, we assembled branched actin networks in vitro from purified components and used simultaneous fluorescence and atomic force microscopy to quantify their molecular composition and material properties under various forces. Remarkably, mechanical loading of these self-assembling materials increases their density, power, and efficiency. Microscopically, increased density reflects increased filament number and altered geometry, but no change in average length. Macroscopically, increased density enhances network stiffness and resistance to mechanical failure beyond those of isotropic actin networks. These effects endow branched actin networks with memory of their mechanical history that shapes their material properties and motor activity. This work reveals intrinsic force feedback mechanisms by which mechanical resistance makes self-assembling actin networks stiffer, stronger, and more powerful
Towards a five-minute comprehensive cardiac MR examination using highly accelerated parallel imaging with a 32-element coil array: Feasibility and initial comparative evaluation
PURPOSE: To evaluate the feasibility and perform initial comparative evaluations of a 5-minute comprehensive whole-heart magnetic resonance imaging (MRI) protocol with four image acquisition types: perfusion (PERF), function (CINE), coronary artery imaging (CAI), and late gadolinium enhancement (LGE). MATERIALS AND METHODS: This study protocol was Health Insurance Portability and Accountability Act (HIPAA)-compliant and Institutional Review Board-approved. A 5-minute comprehensive whole-heart MRI examination protocol (Accelerated) using 6-8-fold-accelerated volumetric parallel imaging was incorporated into and compared with a standard 2D clinical routine protocol (Standard). Following informed consent, 20 patients were imaged with both protocols. Datasets were reviewed for image quality using a 5-point Likert scale (0 = non-diagnostic, 4 = excellent) in blinded fashion by two readers. RESULTS: Good image quality with full whole-heart coverage was achieved using the accelerated protocol, particularly for CAI, although significant degradations in quality, as compared with traditional lengthy examinations, were observed for the other image types. Mean total scan time was significantly lower for the Accelerated as compared to Standard protocols (28.99 +/- 4.59 min vs. 1.82 +/- 0.05 min, P < 0.05). Overall image quality for the Standard vs. Accelerated protocol was 3.67 +/- 0.29 vs. 1.5 +/- 0.51 (P < 0.005) for PERF, 3.48 +/- 0.64 vs. 2.6 +/- 0.68 (P < 0.005) for CINE, 2.35 +/- 1.01 vs. 2.48 +/- 0.68 (P = 0.75) for CAI, and 3.67 +/- 0.42 vs. 2.67 +/- 0.84 (P < 0.005) for LGE. Diagnostic image quality for Standard vs. Accelerated protocols was 20/20 (100%) vs. 10/20 (50%) for PERF, 20/20 (100%) vs. 18/20 (90%) for CINE, 18/20 (90%) vs. 18/20 (90%) for CAI, and 20/20 (100%) vs. 18/20 (90%) for LGE. CONCLUSION: This study demonstrates the technical feasibility and promising image quality of 5-minute comprehensive whole-heart cardiac examinations, with simplified scan prescription and high spatial and temporal resolution enabled by highly parallel imaging technology. The study also highlights technical hurdles that remain to be addressed. Although image quality remained diagnostic for most scan types, the reduced image quality of PERF, CINE, and LGE scans in the Accelerated protocol remain a concern