Hybrid gene misregulation in multiple developing tissues within a recent adaptive radiation of Cyprinodon pupfishes.

Genetic incompatibilities constitute the final stages of reproductive isolation and speciation, but little is known about incompatibilities that occur within recent adaptive radiations among closely related diverging populations. Crossing divergent species to form hybrids can break up coadapted variation, resulting in genetic incompatibilities within developmental networks shaping divergent adaptive traits. We crossed two closely related sympatric Cyprinodon pupfish species-a dietary generalist and a specialized molluscivore-and measured expression levels in their F1 hybrids to identify regulatory variation underlying the novel craniofacial morphology found in this recent microendemic adaptive radiation. We extracted mRNA from eight day old whole-larvae tissue and from craniofacial tissues dissected from 17-20 day old larvae to compare gene expression between a total of seven F1 hybrids and 24 individuals from parental species populations. We found 3.9% of genes differentially expressed between generalists and molluscivores in whole-larvae tissues and 0.6% of genes differentially expressed in craniofacial tissue. We found that 2.1% of genes were misregulated in whole-larvae hybrids whereas 19.1% of genes were misregulated in hybrid craniofacial tissues, after correcting for sequencing biases. We also measured allele specific expression across 15,429 heterozygous sites to identify putative compensatory regulatory mechanisms underlying differential expression between generalists and molluscivores. Together, our results highlight the importance of considering misregulation as an early indicator of genetic incompatibilities in the context of rapidly diverging adaptive radiations and suggests that compensatory regulatory divergence drives hybrid gene misregulation in developing tissues that give rise to novel craniofacial traits

Vitamin D deficiency and segregation status in prisoners

Purpose: The purpose of this paper is to investigate if any exposure to segregation minimal association in a single male prison population had any association with an increased risk of vitamin D deficiency. Design/methodology/approach: A retrospective case study was undertaken with all inmates who had a 25-hyrdoxy-vitamin D test taken during the study period deemed eligible. Hand searching of the medical records by an independent party identified eligible participants whose data were recorded for analysis. Findings: In total, 124 prisoners were deemed eligible for inclusion; 67 were vitamin D sufficient and 57 were vitamin D deficient by Australian standards. Time in segregation minimal association was shown not to be significant, however, smoking (OR 2.93, 95% CI 1.27-6.81, p=0.012) and having Asian ethnicity (OR 4.16, 95% CI 1.56-11.10, p=0.004) independently significantly increased the risk of vitamin D deficiency. Research limitations/implications: This research is limited by its study design, small sample size and single location. Originality/value: This paper presents the first published research into vitamin D levels in a prison population in Australia, and provides a basis for a larger prospective cohort study

Creating regional crime statistics from administrative data

Abstract: The only publicly-available information on the geographical distribution of crime in New Zealand is offence statistics for police administrative units. We investigate whether existing data can be used to construct geographical crime statistics that correspond to regional councils, territorial authorities, and urban areas. Methods We build experimental output geographies from police stations, the smallest administrative unit for which there are long time series of offence statistics. We develop three rules for assigning police stations to the new geographies: one based on population, one based on land area, and one based on both. We assess the performance of these rules by calculating the proportion of national land area and population that is misclassified, and the number of target units that do not receive at least one police station. We also look at whether regional statistics on serious assaults are sensitive to the choice of allocation rule. Findings The new output geographies approximate the target geographies well. For instance, our preferred rule assigns 96 percent of the national population to the correct territorial authority. Moreover, a case study of serious assaults suggests that most regional crime statistics are not sensitive to the choice of rule. Conclusion The new output geographies perform sufficiently well that they could, if required, be used to produce regional crime statistics

General practice registrars’ intentions for future practice: Implications for rural medical workforce planning

The models of practice that general practice registrars (GPRs) envisage undertaking will affect workforce supply. The aim of this research was to determine practice intentions of current GPRs in a regional general practice training program (Coast City Country General Practice Training). Questionnaires were circulated to 220 GPRs undertaking general practice placements to determine characteristics of ideal practice models and intentions for future practice. Responses were received for 99 participants (45%). Current GPRs intend to work an average of less than eight half-day sessions/week, with male participants intending to work more hours (t(91) = 3.528, P = 0.001). More than one-third of this regional cohort intends to practice in metropolitan centres. Proximity to family and friends was the most important factor influencing the choice of practice location. Men ranked remuneration for work as more important (t (88) = –4.280, P \u3c 0.001) and women ranked the ability to work part-time higher (t(94) = 3.697, P \u3c 0.001). Fee-for-service payment alone, or in combination with capitation, was the preferred payment system. Only 22% of Australian medical graduates intend to own their own practice compared with 52% of international medical graduates (χ2(1) = 8.498, P = 0.004). Future general practitioners (GPs) intend to work fewer hours than current GPs. Assumptions about lifestyle factors, practice models and possible professional roles should be carefully evaluated when developing strategies to recruit GPs and GPRs into rural practice

Andrei Drives Bentley, Boris Drives Lada: Why Corrupt States Have Clean Agencies.

In societies where corruption is rife, why do bureaucrats in some agencies extract more than enough to get rich, while others do not? This dissertation explains why corruption varies across agencies within the same state, when the factors that explain variation across states (e.g. political competition) are weak or absent. In other words, why in corrupt states aren't all agencies equally corrupt? My theory explains how these puzzling cases arise from the interaction between bureaucrats in a hierarchy. They must balance risky collusion necessary to grow the available rents, against competition to take a larger share of these spoils. Characteristics of agencies well-known in the bureaucratic politics literature change the terms of this trade-off, most importantly the degree of information asymmetry between bureaucrats authorizing corruption, and those carrying it out. The greater this asymmetry, the more difficult it is to maintain the collusion necessary to succeed at corruption. I test this and other implications using a novel dataset on Russian public procurement covering five million purchases by 110,000 agencies from 2011-2015.PHDPolitical ScienceUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/135764/1/smcgirr_1.pd

Prevention of osteoporotic refractures in regional Australia

Objective: Clinical guidelines recommend that patients who sustain a minimal trauma fracture (MTF) should receive a bone mineral density (BMD) scan and bisphosphonate (or equivalent) therapy if diagnosed with osteoporosis. A pilot fracture liaison service (FLS) was implemented in regional NSW to improve adherence to the guidelines. Design: Prospective cohort study with an historical control. Setting: Primary care. Participants: Control (n = 47) and cohort (n = 93) groups comprised patients consenting to interview who presented with a MTF to the major referral hospital 4 months before and 12 months after FLS implementation respectively. Main outcome measures: Primary outcome measures were the rates of BMD scans and anti-osteoporotic medication initiation/review after MTF. Hospital admission data were also examined to determine death and refracture rates for all patients presenting during the study period with a primary diagnosis of MTF within 3 years of their initial fracture. Results: Although there was no improvement in BMD scanning rates, the reported rate of medication initiation/review after fracture was significantly higher (P \u3c 0.05) in the FLS cohort. However, once adjusted for age, this association was not significant (P = 0.086). There was a lower refracture rate during the cohort period (P = 0.013), however, there were significantly more deaths (P = 0.035) within 3 years of initial fracture. When deaths were taken into account via competing risk regression, patients in the cohort period were significantly less likely to refracture than those in the control period (Hazard ratio = 0.576, P = 0.032). Conclusions: A rurally based nurse-led FLS was associated with modest improvement after MTF. Consideration should be given to ways to strengthen the model of care to improve outcomes

Alien Registration- Mcgirr, Alexander W. (Presque Isle, Aroostook County)

https://digitalmaine.com/alien_docs/33871/thumbnail.jp