Reducing Prawn-trawl Bycatch in Australia: An Overview and an Example from Queensland

Prawn trawling occurs in most states of Australia in tropical, subtropical, and temperate waters. Bycatch occurs to some degree in all Australian trawl fisheries, and there is pressure to reduce the levels of trawl fishery bycatch. This paper gives a brief overview of the bycatch issues and technological solutions that have been evaluated or adopted in Australian prawn-trawl fi sheries. Turtle excluder devices (TED’s) and bycatch reduction devices (BRD’s) are the principal solutions to bycatch in Australian prawn-trawl fisheries. This paper focuses on a major prawn-trawl fishery of northeastern Australia, and the results of commercial use of TED’s and BRD’s in the Queensland east coast trawl fishery are presented. New industry designs are described, and the status of TED and BRD adoption and regulation is summarized. The implementation of technological solutions to reduce fishery bycatch is assumed generally to assist prawn-trawl fisheries within Australia in achieving legislative requirements for minimal environmental impact and ecological sustainable development

Gas injection into second mode instability on a 7 degree cone at Mach 7

The influence of injection gas type on second mode instabilities is researched on a 7 degree half-angle cone at Mach 7. The wind tunnel model of 594.5 mm length with a sharp nosetip is fitted with a porous aluminium patch that spans 60 degree in azimuth and 25 mm in axial length. Four different gases are injected into the boundary layer flow, namely nitrogen, carbon dioxide, helium and argon. Three different tunnel test conditions with different unit Reynolds numbers provide a variety of amplification levels of the second mode instability at the injection location. Frequency data is obtained using PCB sensors and the density boundary layer thickness is inferred from high speed z-type schlieren images. Early analysis of initial data shows a drop in second mode frequency and second mode power behind the injector. If transition is not caused the effect weakens further downstream. Higher blowing ratios caused a stronger decrease in frequency and also reduced the power in the frequency band more. Larger blowing ratios are required at lower unit Reynolds numbers to achieve the same effect. The observed boundary layer thickness, as inferred from schlieren images, followed comparable dynamics. A larger blowing ratio of the same coolant gas lead to more thickening, while Helium had a significantly stronger total effect than carbon dioxide. Preliminary results suggest that helium injection at a moderate rate causes the second mode peak to be disproportionally damped and its bandpower to be significantly reduced. A strongly simplified analytical model suggest that the mechanical increase in boundary layer thickness alone may not be sufficient to explain all observed outcomes

Successful left gonadal vein to inferior vena cava bypass for symptomatic May-Thurner syndrome

We report the management of symptomatic May-Thurner syndrome refractory to endovascular techniques with left gonadal vein to inferior vena cava bypass. The patient\u27s presentation was exceptional-a young individual with end-stage renal disease status post four failed kidney transplants, dwindling options for dialysis access, and an unusable left thigh arteriovenous graft owing to severe lower extremity edema secondary to common iliac vein compression. Postoperatively, swelling was markedly alleviated and the thigh graft was functional. Discussed are endovascular and venous bypass techniques for management of May-Thurner-associated lesions, as well as approaches to end-stage hemodialysis access salvage

Diagnostic Modelling of an Expansion Tube Operating Condition for a Hypersonic Free Shear Layer Experiment

Computational simulations of the AIR-1 test condition in the University of Illinois’ Hypervelocity Expansion Tube were conducted to verify facility operation and to obtain free stream properties that are otherwise difficult to measure. Two types of simulation were undertaken. The first was a one-dimensional simulation of the entire facility and the second was a hybrid simulation, combining a one-dimensional simulation of the shock tube section with a two-dimensional simulation of the acceleration tube. The one-dimensional simulation matched the experimental data well, however the two-dimensional simulation did not initially match the experimental measurements of shock speed and test gas pitot pressure. Further investigation showed the shock speed discrepancy was consistent with air contamination into the acceleration tube and subsequent two-dimensional simulations assuming 10% air contamination showed reasonable agreement with experimental data. Using data taken from the two-dimensional simulation of the expansion tube as a transient inflow condition, modelling was undertaken of a free shear layer experiment being conducted in the facility. Results from equilibrium, finite rate, and perfect gas models were compared. The finite rate simulation provides the best agreement with experimental Schlieren images, with the simulation capturing the major flow structures seen in experiments

Bony ingrowth potential of 3D-printed porous titanium alloy: a direct comparison of interbody cage materials in an in vivo ovine lumbar fusion model.

Background contextThere is significant variability in the materials commonly used for interbody cages in spine surgery. It is theorized that three-dimensional (3D)-printed interbody cages using porous titanium material can provide more consistent bone ingrowth and biological fixation.PurposeThe purpose of this study was to provide an evidence-based approach to decision-making regarding interbody materials for spinal fusion.Study designA comparative animal study was performed.MethodsA skeletally mature ovine lumbar fusion model was used for this study. Interbody fusions were performed at L2-L3 and L4-L5 in 27 mature sheep using three different interbody cages (ie, polyetheretherketone [PEEK], plasma sprayed porous titanium-coated PEEK [PSP], and 3D-printed porous titanium alloy cage [PTA]). Non-destructive kinematic testing was performed in the three primary directions of motion. The specimens were then analyzed using micro-computed tomography (µ-CT); quantitative measures of the bony fusion were performed. Histomorphometric analyses were also performed in the sagittal plane through the interbody device. Outcome parameters were compared between cage designs and time points.ResultsFlexion-extension range of motion (ROM) was statistically reduced for the PTA group compared with the PEEK cages at 16 weeks (p-value=.02). Only the PTA cages demonstrated a statistically significant decrease in ROM and increase in stiffness across all three loading directions between the 8-week and 16-week sacrifice time points (p-value≤.01). Micro-CT data demonstrated significantly greater total bone volume within the graft window for the PTA cages at both 8 weeks and 16 weeks compared with the PEEK cages (p-value<.01).ConclusionsA direct comparison of interbody implants demonstrates significant and measurable differences in biomechanical, µ-CT, and histologic performance in an ovine model. The 3D-printed porous titanium interbody cage resulted in statistically significant reductions in ROM, increases in the bone ingrowth profile, as well as average construct stiffness compared with PEEK and PSP

Upgrade of the X3 super-orbital expansion tube

Expansion tubes are important facilities for the study of high enthalpy hypersonic flows which avoid the non-equilibrium chemical and thermal effects associated with the flow stagnation intrinsic to reflected shock tunnels. X3 is one of the largest freepiston super-orbital expansion tube in the world with an overall length of approximately 69 m and is capable of generating reentry speed flows equivalent to those experienced during a hyperbolic re-entry trajectory. It was originally built with a twostage free-piston driver to achieve the high compression ratio of a large diameter compression tube without the high construction costs of designing the large diameter tube to be strong enough to resist peak driver pressure loads. However, this arrangement proved difficult in operation. This paper describes the upgrades to X3, in respect to its physical layout. The facility has been recommissioned to incorporate a single-piston driver, a steady expansion nozzle and a new test section. Major changes have been made to the free-piston driver with a re-designed piston and launcher and a new end cap tube which is 200 mm thick to contain driver pressures up to 80 MPa. The re-designed piston introduces an area change at the primary diaphragm, ensuring that the maximum increase in total pressure and temperature can be gained as the driver gas undergoes unsteady expansion from sonic to supersonic conditions. The compression process steadily increases up to Mach 1 at the throat then gains of up to an order of magnitude in total temperature and pressure can be realised as the unsteady expansion process takes over. The area change will also increase test times; with a throat at the primary diaphragm, the piston mechanics can be more readily tuned to minimise reflection of waves off the piston which would otherwise reduce the test time. A new Mach 10 steady expansion nozzle has been developed which has increased the core flow and the test time for appropriate conditions. The dump tank has been replaced with a larger tank and test section giving a larger volume with greater potential for instrumentation

MAID : An effect size based model for microarray data integration across laboratories and platforms

<p>Abstract</p> <p>Background</p> <p>Gene expression profiling has the potential to unravel molecular mechanisms behind gene regulation and identify gene targets for therapeutic interventions. As microarray technology matures, the number of microarray studies has increased, resulting in many different datasets available for any given disease. The increase in sensitivity and reliability of measurements of gene expression changes can be improved through a systematic integration of different microarray datasets that address the same or similar biological questions.</p> <p>Results</p> <p>Traditional effect size models can not be used to integrate array data that directly compare treatment to control samples expressed as log ratios of gene expressions. Here we extend the traditional effect size model to integrate as many array datasets as possible. The extended effect size model (MAID) can integrate any array datatype generated with either single or two channel arrays using either direct or indirect designs across different laboratories and platforms. The model uses two standardized indices, the standard effect size score for experiments with two groups of data, and a new standardized index that measures the difference in gene expression between treatment and control groups for one sample data with replicate arrays. The statistical significance of treatment effect across studies for each gene is determined by appropriate permutation methods depending on the type of data integrated. We apply our method to three different expression datasets from two different laboratories generated using three different array platforms and two different experimental designs. Our results indicate that the proposed integration model produces an increase in statistical power for identifying differentially expressed genes when integrating data across experiments and when compared to other integration models. We also show that genes found to be significant using our data integration method are of direct biological relevance to the three experiments integrated.</p> <p>Conclusion</p> <p>High-throughput genomics data provide a rich and complex source of information that could play a key role in deciphering intricate molecular networks behind disease. Here we propose an extension of the traditional effect size model to allow the integration of as many array experiments as possible with the aim of increasing the statistical power for identifying differentially expressed genes.</p

In vitro culture with gemcitabine augments death receptor and NKG2D ligand expression on tumour cells

Much effort has been made to try to understand the relationship between chemotherapeutic treatment of cancer and the immune system. Whereas much of that focus has been on the direct effect of chemotherapy drugs on immune cells and the release of antigens and danger signals by malignant cells killed by chemotherapy, the effect of chemotherapy on cells surviving treatment has often been overlooked. In the present study, tumour cell lines: A549 (lung), HCT116 (colon) and MCF-7 (breast), were treated with various concentrations of the chemotherapeutic drugs cyclophosphamide, gemcitabine (GEM) and oxaliplatin (OXP) for 24 hours in vitro. In line with other reports, GEM and OXP upregulated expression of the death receptor CD95 (fas) on live cells even at sub-cytotoxic concentrations. Further investigation revealed that the increase in CD95 in response to GEM sensitised the cells to fas ligand treatment, was associated with increased phosphorylation of stress activated protein kinase/c-Jun N-terminal kinase and that other death receptors and activatory immune receptors were co-ordinately upregulated with CD95 in certain cell lines. The upregulation of death receptors and NKG2D ligands together on cells after chemotherapy suggest that although the cells have survived preliminary treatment with chemotherapy they may now be more susceptible to immune cell-mediated challenge. This re-enforces the idea that chemotherapy-immunotherapy combinations may be useful clinically and has implications for the make-up and scheduling of such treatments

How U.S. Ocean Policy and Market Power Can Reform the Coral Reef Wildlife Trade

As the world’s largest importer of marine ornamental species for the aquaria, curio, home décor, and jewelry industries, the United States has an opportunity to leverage its considerable market power to promote more sustainable trade and reduce the effects of ornamental trade stress on coral reefs worldwide. Evidence indicates that collection of some coral reef animals for these trades has caused virtual elimination of local populations, major changes in age structure, and promotion of collection practices that destroy reef habitats. Management and enforcement of collection activities in major source countries such as Indonesia and the Philippines remain weak. Strengthening US trade laws and enforcement capabilities combined with increasing consumer and industry demand for responsible conservation can create strong incentives for improving management in source countries. This is particularly important in light of the March 2010 failure of the parties to the Convention on International Trade in Endangered Species (CITES) to take action on key groups of corals