HIV infection significantly reduces lipoprotein lipase which remains low after 6 months of antiretroviral therapy

Purpose of the study Fractional clearance rate of apolipoprotein B100-containing lipoproteins is reduced in HIV infection before and after antiretroviral (ARV) treatment [1]. We compared lipoprotein lipase (LPL) activity and gene expression in HIV-positive subjects before and 6 months after ARV with HIV-negative controls. Methods Fasting blood post heparin total and hepatic lipase activity,adiponectin, leptin, insulin, glucose, and lipid measurementswere made in 32 HIV-infected and 15 HIVnegative controls. LPL was estimated by subtractinghepatic lipase from total lipase. Adiponectin, LPL andhormone sensitive lipase (HSL) gene expression weremeasured from iliac crest subcutaneous fat biopsies.Patients were tested before, and 6 months after randomisation to AZT/3TC (n = 15) or TDF/FTC (n = 17) with EFV.Between-group comparison was by Mann-Whitney andpaired samples by the Wilcoxon signed rank tests. Summary of results There were no differences in gender, ethnicity, baseline BMI, regional fat distribution (whole body DEXA) and visceral (VAT) and subcutaneous fat (SAT) measured by abdominal CT scans between controls and patients. Trunk fat/BMI ratio, VAT and VAT:SAT ratio significantly increased after 6-month ARV therapy (p = 0.01). There were no differences between groups in serum NEFA,HOMA and leptin levels. Selected other results are shown in Table 1. Conclusion Post heparin lipoprotein lipase activity is reduced in HIV and does not return to control levels after 6 months of ARV therapy. AZT-containing regimens are associated with a greater increase in LPL, LPL gene expression and plasma adiponectin than TDF

GASP II. A MUSE view of extreme ram-pressure stripping along the line of sight: kinematics of the jellyfish galaxy JO201

This paper presents a spatially-resolved kinematic study of the jellyfish galaxy JO201, one of the most spectacular cases of ram-pressure stripping (RPS) in the GASP (GAs Stripping Phenomena in Galaxies with MUSE) survey. By studying the environment of JO201, we find that it is moving through the dense intra-cluster medium of Abell 85 at supersonic speeds along our line of sight, and that it is likely accompanied by a small group of galaxies. Given the density of the intra-cluster medium and the galaxy's mass, projected position and velocity within the cluster, we estimate that JO201 must so far have lost ~50% of its gas during infall via RPS. The MUSE data indeed reveal a smooth stellar disk, accompanied by large projected tails of ionised (Halpha) gas, composed of kinematically cold (velocity dispersion <40km/s) star-forming knots and very warm (>100km/s) diffuse emission which extend out to at least ~50 kpc from the galaxy centre. The ionised Halpha-emitting gas in the disk rotates with the stars out to ~6 kpc but in the disk outskirts becomes increasingly redshifted with respect to the (undisturbed) stellar disk. The observed disturbances are consistent with the presence of gas trailing behind the stellar component, resulting from intense face-on RPS happening along the line of sight. Our kinematic analysis is consistent with the estimated fraction of lost gas, and reveals that stripping of the disk happens outside-in, causing shock heating and gas compression in the stripped tails.Comment: ApJ, revised version after referee comments, 15 pages, 16 figures. The interactive version of Figure 9 can be viewed at web.oapd.inaf.it/gasp/publications.htm

From meadows to milk to mucosa – adaptation of Streptococcus and Lactococcus species to their nutritional environments

Lactic acid bacteria (LAB) are indigenous to food-related habitats as well as associated with the mucosal surfaces of animals. The LAB family Streptococcaceae consists of the genera Lactococcus and Streptococcus. Members of the family include the industrially important species Lactococcus lactis, which has a long history safe use in the fermentative food industry, and the disease-causing streptococci Streptococcus pneumoniae and Streptococcus pyogenes. The central metabolic pathways of the Streptococcaceae family have been extensively studied because of their relevance in the industrial use of some species, as well as their influence on virulence of others. Recent developments in high-throughput proteomic and DNA-microarray techniques, in in vivo NMR studies, and importantly in whole-genome sequencing have resulted in new insights into the metabolism of the Streptococcaceae family. The development of cost-effective high-throughput sequencing has resulted in the publication of numerous whole-genome sequences of lactococcal and streptococcal species. Comparative genomic analysis of these closely related but environmentally diverse species provides insight into the evolution of this family of LAB and shows that the relatively small genomes of members of the Streptococcaceae family have been largely shaped by the nutritionally rich environments they inhabit.

Analgesic Efficacy of Pfannenstiel Incision Infiltration with Ropivacaine 7.5 mg/mL for Caesarean Section

Background. Pain after Caesarean delivery is partly related to Pfannenstiel incision, which can be infiltrated with local anaesthetic solutions. Methods. A double- blind randomized control trial was designed to assess the analgesic efficacy of 7.5 mg/mL ropivacaine solution compared to control group, in two groups of one hundred and forty four parturients for each group, who underwent Caesarean section under spinal anaesthesia: group R (ropivacaine group) and group C (control group). All parturients also received spinal sufentanil (2.5 μg). Results. Ropivacaine infiltration in the Pfannenstiel incision for Caesarean delivery before wound closure leads to a reduction of 30% in the overall consumption of analgesics (348 550 mg for group R versus 504 426 mg for group C with P < .05), especially opioids in the first 24 hours, but also significantly increases the time interval until the first request for an analgesic (4 h 20 min ± 2 h 26 for group R versus 2 h 42 ± 1 h 30 for group C). The P values for the two groups were: P < .0001 for paracetamol, P < .0001 for ketoprofen and P for nalbuphine which was the most significant. There is no significant difference in the threshold of VAS in the two series. Conclusion. This technique can contribute towards a programme of early rehabilitation in sectioned mothers, with earlier discharge from the post-labour suite

The equilibrium flow and mass balance of the Taku Glacier, Alaska 1950?2006

International audienceThe Taku Glacier, Alaska has advanced 7.5 km since the late nineteenth century, while all other primary outlet glaciers of the Juneau Icefield are in retreat. The Juneau Icefield Research Program has completed field work on the Taku Glacier annually since 1946. The collected observations of surface mass balance, glacier velocity and glacier thickness at Profile IV 29 km above the terminus and 4 km above the equilibrium line provide a means to assess the equilibrium nature of the Taku Glacier. Velocity measured over a twelve month span and annual summer velocity measurements completed at a Profile IV from 1950?2006 indicate insignificant variations in velocity seasonally or from year to year. The consistency of velocity over the 56-year period indicates that in the vicinity of the equilibrium line, the flow of the Taku Glacier has been in an equilibrium state. Surface mass balance was positive from 1946?1988 averaging +0.42 m a?1. This led to glacier thickening. From 1988?2006 an important change has occurred and annual balance has been ?0.14 m a?1, and the glacier thickness has ceased increasing along Profile IV. Field measurements of ice depth and surface velocity allow calculation of the volume flux at Profile IV. Volume flux is then compared with the surface balance flux from the region of the glacier above Profile IV, determined annually in the field. Above Profile IV the observed mean surface flux is 5.50×108 m3/a (±5%), while the calculated volume flux range flowing through profile IV is 5.00?5.47×108 m3/a. The mean surface flux has been greater than the volume flux, which has led to slow thickening of the Taku Glacier up to 1988. The thickening has not led to a change in the flow of Taku Glacier at Profile IV

Rotational Dynamics of Vortices in Confined Bose-Einstein Condensates

We derive the frequency of precession and conditions for stability for a quantized vortex in a single-component and a two-component Bose-Einstein condensate. The frequency of precession is proportional to the gradient of the free energy with respect to displacement of the vortex core. In a two-component system, it is possible to achieve a local minimum in the free energy at the center of the trap. The presence of such a minimum implies the existence of a region of energetic stability where the vortex cannot escape and where one may be able to generate a persistent current.Comment: 6 Pages, 6 Figure

Electronic feeding behavioural data as indicators of health status in dairy calves

peer-reviewedThe objectives of this study were (i) to characterise clinical health in dairy calves on an Irish research farm during the artificial calf-rearing period and (ii) to determine whether calves’ pre-weaning intakes and feeding behaviour, recorded by electronic calf feeders, changes in response to incidents of bovine respiratory disease (BRD). Holstein-Friesian (H-F) and Jersey (J) calves were fed by automatic milk replacer (MR) and concentrate feeders. Feeding behaviour, including MR consumption, drinking speed, number of rewarded and unrewarded visits to the feeder as well as concentrate consumption, was recorded by the feeders. A modified version of the Wisconsin calf health scoring criteria chart was used to score calves’ clinical measurements and identify incidences of BRD. Thus, 40% of calves were found to have at least one incident of BRD. Feeding behaviour was altered during incidents of BRD. The number of unrewarded visits to the feeder was reduced, by approximately four visits, for calves with BRD during the 3 d prior to the identification of BRD (P < 0.05) and tended to be reduced during the 7 d following the identification of BRD (P = 0.05), compared with healthy calves. Additionally, calves with BRD had a tendency for reduced net energy intake (approximately 8%) during the 3 d prior to the identification of BRD, compared with healthy calves. Therefore, calf feeding behavioural data, recorded by electronic feeders during the pre-weaning period, can indicate cases of BRD