Dydrogesterone and norethisterone regulate expression of lipoprotein lipase and hormones-sensitive lipase in human subcutaneous abdominal adipocytes

Aim: In premenopausal women, hyper-androgenicity is associated with central obesity and an increased cardiovascular risk. We investigated the effects of dydrogesterone (DYD)(a non-androgenic progestogen) and norethisterone (NET)(an androgenic progestogen) on lipoprotein lipase (LPL), hormone-sensitive lipase (HSL) and glycerol release in adipocytes isolated from subcutaneous abdominal adipose tissue. Methods: Adipose tissue was obtained from 12 non-diabetic women, mean age 51 years (range 37-78) and mean BMI 25.4kg/m2 (range 20.3-26.4). Adipocytes were treated with increasing doses of DYD and NET for 48 hours prior to protein extraction. Effects on lipogenesis and lipolysis were assessed using western blotting to determine the expression of key enzymes, LPL (56kDa) and HSL (84kDa) respectively. Measurement of glycerol release into the medium provided an assessment of lipolytic activity. Results: Expression of LPL was increased by DYD and NET (mean protein expression relative to control ± SEM); with greatest effect at 10-8M for DYD: 2.32±0.51(p0.05). Conclusions: DYD and NET significantly increased LPL expression relative to control whilst significantly reducing HSL expression. At the concentrations studied, similar effects were observed with the androgenic NET and the non-androgenic DYD despite differing effects on the lipid profile when taken in combination with estrogen. Further work in this area may improve knowledge about the effects of different progestogens on body fat distribution and enable progestogen use to be tailored to the individual to achieve maximal benefits

Structure of the Large Magellanic Cloud from 2MASS

We derive structural parameters and evidence for extended tidal debris from star count and preliminary standard candle analyses of the Large Magellanic Cloud based on Two Micron All Sky Survey (2MASS) data. The full-sky coverage and low extinction in K_s presents an ideal sample for structural analysis of the LMC. The star count surface densities and deprojected inclination for both young and older populations are consistent with previous work. We use the full areal coverage and large LMC diameter to Galactrocentric distance ratio to infer the same value for the disk inclination based on perspective. A standard candle analysis based on a sample of carbon long-period variables (LPV) in a narrow color range, 1.6<J-K_s<1.7 allows us to probe the three-dimensional structure of the LMC along the line of sight. The intrinsic brightness distribution of carbon LPVs in selected fields implies that \sigma_M\simlt 0.2^m for this color cut. The sample provides a {\it direct} determination of the LMC disk inclination: $42.3^\circ\pm 7.2^\circ$. Distinct features in the photometric distribution suggest several distinct populations. We interpret this as the presence of an extended stellar component of the LMC, which may be as thick as 14 kpc, and intervening tidal debris at roughly 15 kpc from the LMC.Comment: 24 pages, 9 figures. Submitted to Ap

NGC 3576 and NGC 3603: Two Luminous Southern HII Regions Observed at High Resolution with the Australia Telescope Compact Array

NGC 3576 (G291.28-0.71; l=291.3o, b=-0.7o) and NGC 3603 (G291.58-0.43; l=291.6o, b=-0.5o) are optically visible, luminous HII regions located at distances of 3.0 kpc and 6.1 kpc, respectively. We present 3.4 cm Australian Telescope Compact Array (ATCA) observations of these two sources in the continuum and the H90a, He90a, C90a and H113b recombination lines with an angular resolution of 7" and a velocity resolution of 2.6 km/s. All four recombination lines are detected in the integrated profiles of the two sources. Broad radio recombination lines are detected in both NGC 3576 (DV_{FWHM}>= 50 km/s) and NGC 3603 (DV_{FWHM}>=70 km/s). In NGC 3576 a prominent N-S velocity gradient (~30 km/s/pc) is observed, and a clear temperature gradient (6000 K to 8000 K) is found from east to west, consistent with a known IR color gradient in the source. In NGC 3603, the H90a, He90a and the H113b lines are detected from 13 individual sources. The Y^+ (He/H) ratios in the two sources range from 0.08+/-0.04 to 0.26+/-0.10. We compare the morphology and kinematics of the ionized gas at 3.4 cm with the distribution of stars, 10 micron emission and H_2O, OH, and CH_3OH maser emission. These comparisons suggest that both NGC 3576 and NGC 3603 have undergone sequential star formation.Comment: 24 pages, 12 Postscript figure

The very large G-protein coupled receptor VLGR1: a component of the ankle link complex required for the normal development of auditory hair bundles

Sensory hair bundles in the inner ear are composed of stereocilia that can be interconnected by a variety of different link types, including tip links, horizontal top connectors, shaft connectors, and ankle links. The ankle link antigen is an epitope specifically associated with ankle links and the calycal processes of photoreceptors in chicks. Mass spectrometry and immunoblotting were used to identify this antigen as the avian ortholog of the very large G-protein-coupled receptor VLGR1, the product of the Usher syndrome USH2C (Mass1) locus. Like ankle links, Vlgr1 is expressed transiently around the base of developing hair bundles in mice. Ankle links fail to form in the cochleae of mice carrying a targeted mutation in Vlgr1 (Vlgr1/del7TM), and the bundles become disorganized just after birth. FM1-43 [N-(3-triethylammonium)propyl)-4-(4-(dibutylamino)styryl) pyridinium dibromide] dye loading and whole-cell recordings indicate mechanotransduction is impaired in cochlear, but not vestibular, hair cells of early postnatal Vlgr1/del7TM mutant mice. Auditory brainstem recordings and distortion product measurements indicate that these mice are severely deaf by the third week of life. Hair cells from the basal half of the cochlea are lost in 2-month-old Vlgr1/del7TM mice, and retinal function is mildly abnormal in aged mutants. Our results indicate that Vlgr1 is required for formation of the ankle link complex and the normal development of cochlear hair bundles

Beef production from feedstuffs conserved using new technologies to reduce negative environmental impacts

End of project reportMost (ca. 86%) Irish farms make some silage. Besides directly providing feed for livestock, the provision of grass silage within integrated grassland systems makes an important positive contribution to effective grazing management and improved forage utilisation by grazing animals, and to effective feed budgeting by farmers. It can also contribute to maintaining the content of desirable species in pastures, and to livestock not succumbing to parasites at sensitive times of the year. Furthermore, the optimal recycling of nutrients collected from housed livestock can often be best achieved by spreading the manures on the land used for producing the conserved feed. On most Irish farms, grass silage will remain the main conserved forage for feeding to livestock during winter for the foreseeable future. However, on some farms high yields of whole-crop (i.e. grain + straw) cereals such as wheat, barley and triticale, and of forage maize, will be an alternative option provided that losses during harvesting, storage and feedout are minimised and that input costs are restrained. These alternative forages have the potential to reliably support high levels of animal performance while avoiding the production of effluent. Their production and use however will need to advantageously integrate into ruminant production systems. A range of technologies can be employed for crop production and conservation, and for beef production, and the optimal options need to be identified. Beef cattle being finished indoors are offered concentrate feedstuffs at rates that range from modest inputs through to ad libitum access. Such concentrates frequently contain high levels of cereals such as barley or wheat. These cereals are generally between 14% to 18% moisture content and tend to be rolled shortly before being included in coarse rations or are more finely processed prior to pelleting. Farmers thinking of using ‘high-moisture grain’ techniques for preserving and processing cereal grains destined for feeding to beef cattle need to know how the yield, conservation efficiency and feeding value of such grains compares with grains conserved using more conventional techniques. European Union policy strongly encourages a sustainable and multifunctional agriculture. Therefore, in addition to providing European consumers with quality food produced within approved systems, agriculture must also contribute positively to the conservation of natural resources and the upkeep of the rural landscape. Plastics are widely used in agriculture and their post-use fate on farms must not harm the environment - they must be managed to support the enduring sustainability of farming systems. There is an absence of information on the efficacy of some new options for covering and sealing silage with plastic sheeting and tyres, and an absence of an inventory of the use, re-use and post-use fate of plastic film on farms. Irish cattle farmers operate a large number of beef production systems, half of which use dairy bred calves. In the current, continuously changing production and market conditions, new beef systems must be considered. A computer package is required that will allow the rapid, repeatable simulation and assessment of alternate beef production systems using appropriate, standardised procedures. There is thus a need to construct, evaluate and utilise computer models of components of beef production systems and to develop mathematical relationships to link system components into a network that would support their integration into an optimal system model. This will provide a framework to integrate physical and financial on-farm conditions with models for estimating feed supply and animal growth patterns. Cash flow and profit/loss results will be developed. This will help identify optimal systems, indicate the cause of failure of imperfect systems and identify areas where applied research data are currently lacking, or more basic research is required

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