34 research outputs found
Eficácia de Chenopodium ambrosioides (erva-de-santa-maria) no controle de endoparasitos de Coturnix japônica (codorna japonesa)
Eficácia de Chenopodium ambrosioides (erva-de-santa-maria) no controle de endoparasitos de Gallus gallus (galinha caipira)
Eimeria tenella: Inhibition of development in cell culture by serum from chickens fed anticoccidial drugs
Effects of Monensin Feeding and Withdrawal Time on Growth and Carcass Composition in Broiler Chickens
Efficacy and Compatibility of Amprolium and Carbarsone Against Coccidiosis and Blackhead in Turkeys
Establishing mono-eukaryotic Histomonas meleagridis cultures from in vivo infection contaminated with Tetratrichomonas gallinarum and Blastocystis spp.
<p>SUMMARY The necessity to easily establish Histomonas meleagridis cultures has been underlined extensively by many researchers in order to gain more insights in the biology of H. meleagridis. In addition the occurrence of different protozoa in the caeca of birds impedes, however, the isolation and propagation of H. meleagridis from field outbreaks. Therefore, in a kinetic study using transmission electron microscopy the deleterious effects of adventitious protozoa including Tetratrichomonas gallinarum and Blastocystis spp. on cultured H. meleagridis were examined. To overcome this issue, an easy and successful approach to establish the mono-eukaryotic H. meleagridis culture free of other host's protozoa is proposed. At 10 days post infection, liver lesions of H. meleagridis-infected birds were isolated and inoculated into culture media pre-incubated with caecal bacteria. After 48Â h of incubation, presence of H. meleagridis in the cultures was confirmed through morphological evaluation. Additionally, TEM examination and analysis by PCR amplification of the small subunit rRNA gene could exclude the co-cultivation of T. gallinarum and Blastocystis spp. Furthermore, after successful propagation and maintenance of the cultured H. meleagridis, its pathogenicity was affirmed in an infection experiment in turkeys.</p></p