Fission yeast Lem2 and Man1 perform fundamental functions of the animal cell nuclear lamina

In animal cells the nuclear lamina, which consists of lamins and lamin-associated proteins, serves several functions: it provides a structural scaffold for the nuclear envelope and tethers proteins and heterochromatin to the nuclear periphery. In yeast, proteins and large heterochromatic domains including telomeres are also peripherally localized, but there is no evidence that yeast have lamins or a fibrous nuclear envelope scaffold. Nonetheless, we found that the Lem2 and Man1 proteins of the fission yeast Schizosaccharomyces pombe, evolutionarily distant relatives of the Lap2/Emerin/Man1 (LEM) sub-family of animal cell lamin-associated proteins, perform fundamental functions of the animal cell lamina. These integral inner nuclear membrane localized proteins, with nuclear localized DNA binding Helix-Extension-Helix (HEH) domains, impact nuclear envelope structure and integrity, are essential for the enrichment of telomeres at the nuclear periphery and by means of their HEH domains anchor chromatin, most likely transcriptionally repressed heterochromatin, to the nuclear periphery. These data indicate that the core functions of the nuclear lamina are conserved between fungi and animal cells and can be performed in fission yeast, without lamins or other intermediate filament proteins

The progenitor and early evolution of the Type IIb SN 2016gkg

We report initial observations and analysis on the Type IIb SN~2016gkg in the nearby galaxy NGC~613. SN~2016gkg exhibited a clear double-peaked light curve during its early evolution, as evidenced by our intensive photometric follow-up campaign. SN~2016gkg shows strong similarities with other Type IIb SNe, in particular with respect to the \he~emission features observed in both the optical and near infrared. SN~2016gkg evolved faster than the prototypical Type~IIb SN~1993J, with a decline similar to that of SN~2011dh after the first peak. The analysis of archival {\it Hubble Space Telescope} images indicate a pre-explosion source at SN~2016gkg's position, suggesting a progenitor star with a $\sim$mid F spectral type and initial mass $15-20$\msun, depending on the distance modulus adopted for NGC~613. Modeling the temperature evolution within $5\,\rm{days}$ of explosion, we obtain a progenitor radius of $\sim\,48-124$\rsun, smaller than that obtained from the analysis of the pre-explosion images ($240-320$\rsun).Comment: 7 pages, 5 figures. Submitted to ApJ Letter

Development of a Time Efficient Protocol for Cross-Limb Comparisons of Muscle Mitochondrial Capacity Using NIRS

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SN 2015ba: A type IIP supernova with a long plateau

We present optical photometry and spectroscopy from about a week after explosion to $\sim$272 d of an atypical Type IIP supernova, SN 2015ba, which exploded in the edge-on galaxy IC 1029. SN 2015ba is a luminous event with an absolute V-band magnitude of -17.1$\pm$0.2 mag at 50 d since explosion and has a long plateau lasting for $\sim$123 d. The distance to the SN is estimated to be 34.8$\pm$0.7 Mpc using the expanding photosphere and standard candle methods. High-velocity H-Balmer components constant with time are observed in the late-plateau phase spectra of SN 2015ba, which suggests a possible role of circumstellar interaction at these phases. Both hydrodynamical and analytical modelling suggest a massive progenitor of SN 2015ba with a pre-explosion mass of 24-26 M$_\odot$. However, the nebular spectra of SN 2015ba exhibit insignificant levels of oxygen, which is otherwise expected from a massive progenitor. This might be suggestive of the non-monotonical link between O-core masses and the zero-age main-sequence mass of pre-supernova stars and/or uncertainties in the mixing scenario in the ejecta of supernovae.Comment: 42 pages, 7 pages Appendix, 20 figures, 10 tables, Accepted for publication in MNRAS, 14-June-201

Contractile properties of the muscles of mastication of rhesus monkeys (Macaca mulatta) following increase in muscle length

The hypothesis was tested that increasing the resting length of the masseter and temporalis muscles by a bite-opening appliance with or without detachment and re-attachment of the masseter would not affect the contractile properties of these muscles. Appliances opened the bite of 10 adult female monkeys 20mm. Five received the appliance alone (Group A); five received the appliance and in addition the masseter was detached and re-attached (Group ADR). Comparisons were made 48 weeks later. Small bundles of fibres were excised from the masseter and temporalis muscles of experimental animals and from 8 control animals. Isometric and isotonic contractile properties were measured in vitro and fibre classification and fibre areas were determined histochemically. No significant differences were observed within either masseter or temporalis muscles between animals in Groups A and ADR. In both groups, the bundles of fibres from the masseter had prolonged contraction and relaxation times compared to control masseter muscles but no difference was observed in the percentage of Type II fibres. As detachment and re-attachment had no significant effect on morphological or physiological characteristics, other than those due to lengthening, this procedure may be useful in decreasing the passive tension induced when orthognathic surgery increases muscle length. The significant prolongation of the contractile response of the masseter is similar to the adaptation induced by long-term stimulation at low frequency.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/24139/1/0000396.pd

SN Refsdal: Classification as a Luminous and Blue SN 1987A-like Type II Supernova

We have acquired Hubble Space Telescope (HST) and Very Large Telescope near-infrared spectra and images of supernova (SN) Refsdal after its discovery as an Einstein cross in Fall 2014. The HST light curve of SN Refsdal matches the distinctive, slowly rising light curves of SN 1987A-like supernovae (SNe), and we find strong evidence for a broad H-alpha P-Cygni profile in the HST grism spectrum at the redshift (z = 1.49) of the spiral host galaxy. SNe IIn, powered by circumstellar interaction, could provide a good match to the light curve of SN Refsdal, but the spectrum of a SN IIn would not show broad and strong H-alpha absorption. From the grism spectrum, we measure an H-alpha expansion velocity consistent with those of SN 1987A-like SNe at a similar phase. The luminosity, evolution, and Gaussian profile of the H-alpha emission of the WFC3 and X-shooter spectra, separated by ~2.5 months in the rest frame, provide additional evidence that supports the SN 1987A-like classification. In comparison with other examples of SN 1987A-like SNe, SN Refsdal has a blue B-V color and a high luminosity for the assumed range of potential magnifications. If SN Refsdal can be modeled as a scaled version of SN 1987A, we estimate it would have an ejecta mass of 20+-5 solar masses. The evolution of the light curve at late times will provide additional evidence about the potential existence of any substantial circumstellar material (CSM). Using MOSFIRE and X-shooter spectra, we estimate a subsolar host-galaxy metallicity (8.3+-0.1 dex and <8.4 dex, respectively) near the explosion site.Comment: Submitted to ApJ; 26 page