Proteomic Signature of the Murine Intervertebral Disc

Low back pain is the most common musculoskeletal problem and the single most common cause of disability, often attributed to degeneration of the intervertebral disc. Lack of effective treatment is directly related to our limited understanding of the pathways responsible for maintaining disc health. While transcriptional analysis has permitted initial insights into the biology of the intervertebral disc, complete proteomic characterization is required. We therefore employed liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) protein/peptide separation and mass spectrometric analyses to characterize the protein content of intervertebral discs from skeletally mature wild-type mice. A total of 1360 proteins were identified and categorized using PANTHER. Identified proteins were primarily intracellular/plasma membrane (35%), organelle (30%), macromolecular complex (10%), extracellular region (9%). Molecular function categorization resulted in three distinct categories: catalytic activity (33%), binding (molecule interactions) (29%), and structural activity (13%). To validate our list, we confirmed the presence of 14 of 20 previously identified IVD-associated markers, including matrix proteins, transcriptional regulators, and secreted proteins. Immunohistochemical analysis confirmed distinct localization patterns of select protein with the intervertebral disc. Characterization of the protein composition of healthy intervertebral disc tissue is an important first step in identifying cellular processes and pathways disrupted during aging or disease progression

Benchmarking the Delivery of FEMA Training to Operation Line Employees within Surface Transit Agencies

In February 2003, in the aftermath of the September 11, 2001 terror attacks, the administration of then President George W. Bush developed Homeland Security Presidential Directive-5 (HSPD-5), which mandated the use of the National Incident Management System (NIMS), and the Incident Command System (ICS) by all agencies receiving federal funding (Bush, 2003). The intent of HSPD-5 was to “enhance the ability of the United States to manage domestic incidents by establishing a single, comprehensive national incident management system” (Bush, 2003), or in other words, to ensure that emergency response by the many disparate agencies under the umbrella of government funding is using the same tools, resources, and operating concepts when they respond to an event; an alignment of mission. Because surface transit agencies fall under the HSPD-5 mandate and are an essential pillar in state, local, and national emergency response, it is important to understand how these agencies are presently implementing the HSPD-5 mandate to operation line employees receiving ICS 100/200 training. Therefore, this paper will identify the current delivery method employed for FEMA mandated Incident Command System (ICS) 100/200 training (e.g. online-only, face-to-face, or blended) for operation line employees at a few select surface transit agencies from broadly similarly sized metro areas, in different regions of the United States, and how the delivery of this training can be made more effective. The specific surface transportation agencies that will be examined are the Santa Clara County Valley Transit Authority (SCCVTA), the Southeastern Pennsylvania Transit Authority (SEPTA), the Delaware River Port Authority (DRPA), and the Pinellas Suncoast Transit Authority (PSTA). These agencies were chosen because they service a broadly similar number of citizens, exhibit a distinct geographical and regional diversity, and because of the different threats that they face based on the disparate geographic regions from which they operate. These factors will allow for a more general assessment of NIMS/ICS implementation, and what factors, if any, serve to impede implementation as well as issues related to the deployment of NIMS/ICS in the event of a real-world scenario. In assessing the ability to deploy NIMS/ICS effectively in the field this document will examine the perceived efficacy of various teaching methodologies: online-only, face-to-face, or blended. Finally, the paper will seek to determine the agency’s satisfaction with the current training methods employed by the agency, as well as identify any factors that might qualitatively improve ICS 100/200 learner outcomes; what they might change about current training. Ultimately, the goal is to increase understanding of the ICS 100/200 training methods employed by transit agencies, identify areas that might be suggestive of improvement, and provide recommendations that align with both the mandate and the needs of the organizations

Homeownership and Social Capital in New Zealand

Does homeownership affect individual social capital and thereby influence local outcomes? Following DiPasquale and Glaeser, a body of literature suggests that homeownership is positively related to social capital formation. Homeowners have an incentive to engage in the local community in order to preserve or enhance the value of their housing asset. Moreover, homeownership creates barriers to geographic mobility, which increases the present value of the expected stream of benefits from local community social capital. We test the homeownership hypothesis alongside other individual, household and locational determinants of social capital using unique data created by merging the 2006 and 2008 samples of the New Zealand Quality of Life survey. The measures of social capital used in our analysis include trust in others, participation in social networks, attitude towards local governance and sense of community. Since homeownership is not randomly assigned, we complement our regression models with propensity score matching to control for selection effects. The results confirm that homeownership exerts considerable positive impact in the formation of social capital in New Zealand communities. In raising accountability of local government it does, however, lead to reduced satisfaction by homeowners in the performance of local councils.social capital, homeownership, New Zealand, matching methods

Social capital and regional social infrastructure investment: Evidence from New Zealand

In this paper we link unique data on local social infrastructure expenditure with micro-level individual survey data of self-reported social capital measures of trust and participation in community activities. We use both probit and tobit models to estimate the impact of social infrastructure expenditure on social capital formation. Our results imply that the links between social capital, demographic characteristics, human capital, geography and public social infrastructure investment are rather more subtle and complex than much of the literature implies. While we find evidence in support of many of the hypothesized relationships discussed in the social capital literature, our results also suggest that the impact of public social infrastructure investment is affected by both selection effects and free rider processes.Social capital, trust, participation, public infrastructure, demography, geography

Cleveland Opportunity Corridor Project Reader-Friendly Environmental Impact Statement

How do we make technical information understandable and appealing to a non-technical audience? The Cleveland Opportunity Corridor project in Ohio involves building an urban boulevard with traffic signals to connect Cleveland’s interstate system to University Circle—one of the city’s major economic centers and employment hubs. The HNTB Corporation wrote a reader-friendly EIS that is practical, easy to understand, and visually appealing. This presentation outlines the motivation for developing such a document and how to create one

Notochord cells in intervertebral disc development and degeneration

© 2015 by the authors. All right reserved. The intervertebral disc is a complex structure responsible for flexibility, multi-axial motion, and load transmission throughout the spine. Importantly, degeneration of the intervertebral disc is thought to be an initiating factor for back pain. Due to a lack of understanding of the pathways that govern disc degeneration, there are currently no disease-modifying treatments to delay or prevent degenerative disc disease. This review presents an overview of our current understanding of the developmental processes that regulate intervertebral disc formation, with particular emphasis on the role of the notochord and notochord-derived cells in disc homeostasis and how their loss can result in degeneration. We then describe the role of small animal models in understanding the development of the disc and their use to interrogate disc degeneration and associated pathologies. Finally, we highlight essential development pathways that are associated with disc degeneration and/or implicated in the reparative response of the tissue that might serve as targets for future therapeutic approaches

Findings and Implications from a Project on White Supremacist Entry and Exit Pathways

This Research Note provides an overview of the main findings from a project on white supremacist pathways - or why some individuals join and leave white supremacist groups - with a specific focus on elucidating common themes, theoretical applications, main takeaways, and providing recommendations for academics and policymakers. One key lesson is that identity is central to entry and exit pathways

Routine resite of peripheral intravenous devices every 3 days did not reduce complications compared with clinically indicated resite: a randomised controlled trial

<p>Abstract</p> <p>Background</p> <p>Peripheral intravenous device (IVD) complications were traditionally thought to be reduced by limiting dwell time. Current recommendations are to resite IVDs by 96 hours with the exception of children and patients with poor veins. Recent evidence suggests routine resite is unnecessary, at least if devices are inserted by a specialised IV team. The aim of this study was to compare the impact of peripheral IVD 'routine resite' with 'removal on clinical indication' on IVD complications in a general hospital without an IV team.</p> <p>Methods</p> <p>A randomised, controlled trial was conducted in a regional teaching hospital. After ethics approval, 362 patients (603 IVDs) were randomised to have IVDs replaced on clinical indication (185 patients) or routine change every 3 days (177 patients). IVDs were inserted and managed by the general hospital medical and nursing staff; there was no IV team. The primary endpoint was a composite of IVD complications: phlebitis, infiltration, occlusion, accidental removal, local infection, and device-related bloodstream infection.</p> <p>Results</p> <p>IVD complication rates were 68 per 1,000 IVD days (clinically indicated) and 66 per 1,000 IVD days (routine replacement) (<it>P </it>= 0.86; HR 1.03; 95% CI, 0.74-1.43). Time to first complication per patient did not differ between groups (KM with log rank, <it>P </it>= 0.53). There were no local infections or IVD-related bloodstream infections in either group. IV therapy duration did not differ between groups (<it>P </it>= 0.22), but more (<it>P </it>= 0.004) IVDs were placed per patient in the routine replacement (mean, 1.8) than the clinical indication group (mean, 1.5), with significantly higher hospital costs per patient (<it>P </it>< 0.001).</p> <p>Conclusions</p> <p>Resite on clinical indication would allow one in two patients to have a single cannula per course of IV treatment, as opposed to one in five patients managed with routine resite; overall complication rates appear similar. Clinically indicated resite would achieve savings in equipment, staff time and patient discomfort. There is growing evidence to support the extended use of peripheral IVDs with removal only on clinical indication.</p> <p>Registration number</p> <p>Australian New Zealand Clinical Trials Registry (ANZCTR) Number ACTRN12608000421336.</p

Development and validation of a protocol to identify and recruit participants into a large scale study on liver fluke in cattle

Abstract Background Liver fluke infection caused by the parasite Fasciola hepatica is a major cause of production losses to the cattle industry in the UK. To investigate farm-level risk factors for fluke infection, a randomised method to recruit an appropriate number of herds from a defined geographical area into the study was required. The approach and hurdles that were encountered in designing and implementing this study are described. The county of Shropshire, England, was selected for the study because of the variation between farms in exposure to fluke infection observed in an earlier study. Results From a sampling list of 569 holdings in Shropshire randomly drawn from the RADAR cattle population dataset, 396 (69.6%) holdings were successfully contacted by telephone and asked if they would be interested in taking part in the study. Of 296 farmers who agreed to receive information packs by post, 195 (65.9%) agreed to take part in the study. Over the period October 2014 – April 2015 visits were made to 100 dairy and 95 non-dairy herds. During the farm visits 40 faecal samples +/− bulk-tank milk samples were collected and a questionnaire administered. Composite faecal samples were analysed for the presence of F. hepatica eggs by sedimentation and bulk tank milk samples were tested with an antibody ELISA for F. hepatica. Forty-five (49%) of non-dairy herds were positive for liver fluke infection as determined by the finding of one or more fluke eggs, while 36 (36%) dairy herds had fluke positive faecal samples and 41 (41%) dairy herds were positive for F. hepatica antibody. Eighty-seven (45.8%) farmers said that they monitored their cattle for liver fluke infection and 118 (62.1%) reported that they used flukicide drugs in their cattle. Conclusions Using a protocol of contacting farmers directly by telephone and subsequently sending information by post, 79% of the target sample size was successfully recruited into the study. A dataset of farm-specific information on possible risk factors for liver fluke infection and corresponding liver-fluke infection status was generated for the development of statistical models to identify risk factors for liver fluke infection at the farm-level