Rapid identification of Corynebacterium diphtheriae clonal group associated with diphtheria epidemic, Russian Federation.

We used 199 Corynebacterium diphtheriae isolated from 1995 to 1997 in Russia to evaluate the ability of random amplified polymorphic DNA (RAPD) to identify the unique clonal group that emerged there in 1990. Our data show that RAPD can reliably, reproducibly, and rapidly screen a large number of strains to identify the epidemic clonal group

Studies of the Composition of the Cell Antigen Preparations Obtained from Pathogenic Burkholderia, Using Immunoblotting

Carried out has been comparative analysis of the cell antigen immune-electrophoregrammes of B. pseudomallei C-141, B. mallei 10230, B. thailandensis 264, and B. cepacia 25416 after immunoblotting with immune rabbit sera to living cells of avirulent B. pseudomallei 107, celllular and extracellular antigens of B. pseudomallei 107, B. pseudomallei C-141, B. mallei 10230, B. thailandensis 264, and B. cepacia 25416. Visual investigation has revealed the presence of fractions with molar mass of 18.4 and 35 kDa in B. malei 10230 cell antigens’ electrophoregramme obtained by means of immunoblotting with serum to B. malei 10230 cell antigens, as distinct from B. pseudomallei C-141 electrophoregramme with the same serum. This makes it possible to distinguish the pathogenic Burkholderia. At the same time utilization of heterologous immune sera enhances discriminating capacity of the comparative assay. The level of reproducibility of immune-electrophoregrammes allows for the deployment of the similarity coefficient for computer-based comparative analysis

Forecasting Electricity Demand in the Russian Federation and Its Regions Taking Into Account Electrification Expansion

A global priority of electrification expansion in all areas is also stated in the Energy Strategy of the Russian Federation. The study aims to forecast electricity demand in Russia taking into account possible electrification options in economic sectors. The article presents a multi-stage procedure and a concise review of methodological approaches to the long-term assessment of electricity demand, which considers the impact of complex interrelationships in social, economic and technological policies. In particular, this approach focuses on the regional level, where the interests of energy producers and consumers are reconciled. The current and promising directions of electricity use in Russian regions and economic sectors were analysed based on various statistics and forecasts. The conducted analysis demonstrated the stability of sectoral and territorial energy consumption patterns, as well as a decrease and convergence of values of gross regional product (GRP) energy intensity. Energy consumption of regions varies due to significant differences in industrial specialisation and living standards of the population. The highest energy consumption is observed in developed regions (Central Federal District) or regions with a large share of energy-intensive industries (Siberian Federal District). According to the accepted economic development and electrification expansion strategies for the period 2025–2040, on average, electricity demand in Russian regions is expected to increase by 1.4–1.8 % annually. It is anticipated that the Siberian and Far Eastern Federal Districts will show the highest growth rates of energy consumption due to the accelerated development of these territories. Predicted dynamics of the gross domestic product (GDP) energy intensity in Russia confirms its compliance with global trends. The research findings may prove useful in creating programmes and development strategies for the country and its regions.

Forecasting Electricity Demand in the Russian Federation and Its Regions Taking Into Account Electrification Expansion

A global priority of electrification expansion in all areas is also stated in the Energy Strategy of the Russian Federation. The study aims to forecast electricity demand in Russia taking into account possible electrification options in economic sectors. The article presents a multi-stage procedure and a concise review of methodological approaches to the long-term assessment of electricity demand, which considers the impact of complex interrelationships in social, economic and technological policies. In particular, this approach focuses on the regional level, where the interests of energy producers and consumers are reconciled. The current and promising directions of electricity use in Russian regions and economic sectors were analysed based on various statistics and forecasts. The conducted analysis demonstrated the stability of sectoral and territorial energy consumption patterns, as well as a decrease and convergence of values of gross regional product (GRP) energy intensity. Energy consumption of regions varies due to significant differences in industrial specialisation and living standards of the population. The highest energy consumption is observed in developed regions (Central Federal District) or regions with a large share of energy-intensive industries (Siberian Federal District). According to the accepted economic development and electrification expansion strategies for the period 2025–2040, on average, electricity demand in Russian regions is expected to increase by 1.4–1.8 % annually. It is anticipated that the Siberian and Far Eastern Federal Districts will show the highest growth rates of energy consumption due to the accelerated development of these territories. Predicted dynamics of the gross domestic product (GDP) energy intensity in Russia confirms its compliance with global trends. The research findings may prove useful in creating programmes and development strategies for the country and its regions.

ROC-Analysis of the Results as regards <I>B. pseudomallei</I> and <I>B. mallei</I> Agents Detection Using Solid-Phase ELISA

Analyzed are operational characteristic curves (ROC-curves) of solid-phase ELISA variants with immune sera to cellular and extracellular Burkholderia antigens with the aim to determine the most effective ones for B. pseudomallei and B. mallei antigen detection. It is shown that among solid-phase ELISA variants with immune sera to cellular and extracellular antigens, those with sera to homologous antigens are the most effective. Demonstrated is the possibility of application of solid-phase ELISA variants with immune sera to antigens of B. thailandensis and B. cepacia strains for identification and typing of glanders and melioidosis agents. Assessed are prospects of extracellular antigen application for differentiation between closely related species of “ pseudomallei ” group

Size fractionation of trace elements in the surface water and groundwater of the Ganjiang River and Xiushui River basins, China

The research of trace element behaviour and size fractionation is significant to understand the processes in the water-rock system. Moreover, the aqueous speciation of trace elements is strongly related to their distribution and toxicity. In this research, trace elements behaviour and their size fractionation are investigated in the natural water (the surface water and shallow groundwater) of the Ganjiang River and Xiushui River basins. Trace element concentrations were measured by ICP-MS. The authors estimated the size fractionation of the trace elements between suspended forms (>0.45 [mu]m) and dissolved fractions, which included truly dissolved (1 kDa) and colloidal particles (0.45 [mu]m-1 kDa)), after filtration through a 0.45 [mu]m acetate cellulose membrane filter. The distinct features of the trace element size fractionation were identified. The size fractionation of the trace elements under different conditions (in the surface water and shallow groundwater) was studied

FEATURES OF GENOTYPiNG VARIABILITY OF STRAINS OF BORDETELLA PERTUSSIS ALLOCATED FROM PATIENTS WITH WHOOPING COUGH IN RUSSIA

On the basis of studying of features of structure of 7 genes determining major factors of pathogenicity of the causative agent of whooping cough - ptxA, ptxB, ptxC, ptxD, ptxE, ptxP and prn, features of genotyping variability of strains of B.pertussis allocated from patients with whooping cough in Russia are shown. Dynamics of formation of population of strains of the causative agent of whooping cough is tracked and is established that population of strains of B.pertussis is formed by clonal expansion of strains with, new genetic structure of major factors of pathogenicity - pertussis toxin and. pertactin. The structure of population of strains of B.pertussis causing a disease by whooping cough, at the present stage of epidemic process of a pertussis infection - domination. of strains with new «non-vaccine» alleles genes - ptxA1 (97,7 %), ptxC2 (87,4 %), prn2 (89,5 %) and. ptxP3 (93,3 %), in 2,2 % cases with ptxB2 allele and. in 1,3 % cases with. prn9 allele circulate

National Report for the IAG of the IUGG 2019-2022

Major results of researches conducted by Russian geodesists in 2019-2022 on the topics of the International Association of Geodesy (IAG) of the International Union of Geodesy and Geophysics (IUGG) are presented in this issue. This report is prepared by the Section of Geodesy of the National Geophysical Committee of Russia. In the report prepared for the XXVII General Assembly of IUGG (Germany, Berlin, 11-20 July 2023), the results of principal researches in geodesy, geodynamics, gravimetry, in the studies of geodetic reference frame creation and development, Earth's shape and gravity field, Earth's rotation, geodetic theory, its application and some other directions are briefly described. For some objective reasons not all results obtained by Russian scientists on the field of geodesy are included in the report.Comment: Misprint in the title of the arXiv record has been corrected. The submission content is not affecte

Forecasting Electricity Demand in the Russian Federation and Its Regions Taking Into Account Electrification Expansion

Расширение электрификации всех сфер деятельности признано одним из ключевых приоритетов в мире и нашло отражение в Энергетической стратегии России. Целью настоящего исследования является перспективная оценка спроса на электроэнергию в стране при реализации возможных направлений электрификации в секторах экономики. В статье представлены поэтапная схема и краткое описание моделей методического подхода к долгосрочной оценке спроса на энергоносители, который позволяет учесть влияние сложных взаимосвязей в социальной, экономической и технологической политике. Особенностью подхода является акцент исследований на уровне регионов, где происходит согласование интересов производителей и потребителей энергии. Приводится анализ современного состояния и перспективных направлений использования электроэнергии в разных секторах экономики РФ и ее регионах с использованием новых статистических данных и прогнозов. Показано, что секторальная и территориальная структура электропотребления отличаются стабильностью, наблюдаются снижение и сближение значений электроемкости ВРП регионов, а электропотребление регионов различается из-за существенных особенностей в отраслевой специализации и уровне жизни населения. Яаибольшее электропотребление приходится на развитые регионы (Центральный ФО), либо регионы с большой долей электроемких производств (Сибирский ФО). В соответствии с принятым сценарием развития экономики и расширением электрификации на период 2025-2040 гг., можно ожидать, что спрос на электроэнергию в регионах будет увеличиваться со среднегодовыми темпами прироста 1,4-1,8 %. Яаибольшие темпы прироста электропотребления ожидаются в Сибирском ФО и Дальневосточном ФО, они обусловлены ускоренным развитием этих территорий. Прогноз динамики электроемкости ВВП России демонстрирует соответствие глобальным тенденциям. Полученные результаты могут использоваться в качестве ориентира при разработке программ и стратегий развития экономики и энергетики страны и регионов.A global priority of electrification expansion in all areas is also stated in the Energy Strategy of the Russian Federation. The study aims to forecast electricity demand in Russia taking into account possible electrification options in economic sectors. The article presents a multi-stage procedure and a concise review of methodological approaches to the long-term assessment of electricity demand, which considers the impact of complex interrelationships in social, economic and technological policies. In particular, this approach focuses on the regional level, where the interests of energy producers and consumers are reconciled. The current and promising directions of electricity use in Russian regions and economic sectors were analysed based on various statistics and forecasts. The conducted analysis demonstrated the stability of sectoral and territorial energy consumption patterns, as well as a decrease and convergence of values of gross regional product (GRP) energy intensity. Energy consumption of regions varies due to significant differences in industrial specialisation and living standards of the population. The highest energy consumption is observed in developed regions (Central Federal District) or regions with a large share of energy-intensive industries (Siberian Federal District). According to the accepted economic development and electrification expansion strategies for the period 2025-2040, on average, electricity demand in Russian regions is expected to increase by 1.4-1.8 % annually. It is anticipated that the Siberian and Far Eastern Federal Districts will show the highest growth rates of energy consumption due to the accelerated development of these territories. Predicted dynamics of the gross domestic product (GDP) energy intensity in Russia confirms its compliance with global trends. The research findings may prove useful in creating programmes and development strategies for the country and its regions.Исследование выполнено в рамках проекта государственного задания (№ FWEU-2021-0003 рег. номер АААА-А21-121012090014-5) программы фундаментальных исследований РФ на 2021-2030 гг. и при частичной финансовой поддержке РФФИ научных проектов № 18-010-00176 и № 20-010-00204.The article has been prepared in the framework of the State Assignment Project (No. FWEU-2021-0003 Reg. No. АААА-А21-121012090014-5) of the Fundamental Research Program of the Russian Federation 2021-2030; it was also partially financially supported by the Russian Foundation for Basic Research (the projects No. 18-010-00176 and No. 20-010-00204)