7 research outputs found
Π‘ΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ Π²ΠΈΠ΄ΠΎΠ² ΡΡΠΈΠΏΡΠΎΠ² Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ PCR-RFLP ΠΈ ΠΏΡΡΠΌΠΎΠ³ΠΎ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ
The study reveals diagnosis peculiarities for main Thrips spp. occurring during phytosanitary testing. Morphological diagnostic methods were compared with PCR-RFLP and direct sequencing. High reliability of molecular and genetic methods has been shown. These methods provide for determining the species of thrips larvae and damaged specimens of imagoes.Π ΡΠ°Π±ΠΎΡΠ΅ ΠΈΠ·Π»ΠΎΠΆΠ΅Π½Ρ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
Π²ΠΈΠ΄ΠΎΠ² ΡΡΠΈΠΏΡΠΎΠ², Π²ΡΡΡΠ΅ΡΠ°ΡΡΠΈΡ
ΡΡ Π² ΡΠΈΡΠΎΡΠ°Π½ΠΈΡΠ°ΡΠ½ΠΎΠΉ ΡΠΊΡΠΏΠ΅ΡΡΠΈΠ·Π΅. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Ρ PCR-RFLP ΠΈ ΠΏΡΡΠΌΡΠΌ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ. ΠΠΎΠΊΠ°Π·Π°Π½Π° Π²ΡΡΠΎΠΊΠ°Ρ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎΡΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΡΡΡΠ°Π½Π°Π²Π»ΠΈΠ²Π°ΡΡ Π²ΠΈΠ΄ΠΎΠ²ΡΡ ΠΏΡΠΈΠ½Π°Π΄Π»Π΅ΠΆΠ½ΠΎΡΡΡ Π»ΠΈΡΠΈΠ½ΠΎΠΊ ΡΡΠΈΠΏΡΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½Π½ΡΡ
ΠΈΠΌΠ°Π³ΠΎ
Excavation of an early 17th-century glassmaking site at Glasshouse, Shinrone, Co. Offaly, Ireland
An archaeological research excavation was conducted in the area immediately surrounding an upstanding glassmaking furnace near Shinrone, Co. Offaly, Ireland. It dates to the early to mid 17th century and was built and operated by French Huguenots, probably de Hennezells (de Hennezel/Henzeys/Hensie) who had settled in this region as part of the Crown plantation of Kingβs County (now Co. Offaly). This furnace, which employed wood rather than coal as a fuel, is a very rare survival, with no other upstanding examples known in Ireland, Britain or the Lorraine region of France where the form probably originated
ΠΠΎΠ½ΡΡΠΎΠ»Ρ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎΡΡΠΈ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΡΠΈΡΠΎΡΠ°Π½ΠΈΡΠ°ΡΠ½ΠΎΠΉ ΡΠΊΡΠΏΠ΅ΡΡΠΈΠ·Ρ ΠΏΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ
An internal PCR control system based on a mouse gene fragment cloned into a vector was developed. The created internal control system allowed for choosing a method for DNA extraction from bio-baits which doesn't inhibit the amplification process when Phytophthora sp. is being diagnosed. During the process of diagnosing the pathogen of the ring rot of potato, it was shown that the developed internal control in real-time PCR turned out to be more informative than the mitochondrial gene of a host plant used for that purpose.Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΡΠΈΡΡΠ΅ΠΌΠ° Π²Π½ΡΡΡΠ΅Π½Π½Π΅Π³ΠΎ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ (ΠΠ) ΠΏΡΠΎΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ ΠΠ¦Π Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ° Π³Π΅Π½Π° ΠΌΡΡΠΈ, Π²ΡΡΡΠΎΠ΅Π½Π½ΠΎΠ³ΠΎ Π² Π²Π΅ΠΊΡΠΎΡ. Π‘ΠΎΠ·Π΄Π°Π½Π½Π°Ρ ΡΠΈΡΡΠ΅ΠΌΠ° ΠΠ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»Π° Π²ΡΠ±ΡΠ°ΡΡ ΠΌΠ΅ΡΠΎΠ΄ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΡ ΠΠΠ ΠΈΠ· Π±ΠΈΠΎΠΏΡΠΈΠΌΠ°Π½ΠΎΠΊ, Π½Π΅ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠΈΠΉ ΠΏΡΠΎΡΠ΅ΡΡ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΠΏΡΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ Phytophthora sp. ΠΡΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ Π²ΠΎΠ·Π±ΡΠ΄ΠΈΡΠ΅Π»Ρ ΠΊΠΎΠ»ΡΡΠ΅Π²ΠΎΠΉ Π³Π½ΠΈΠ»ΠΈ ΠΊΠ°ΡΡΠΎΡΠ΅Π»Ρ Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΠΉ ΠΠ Π² ΡΠ΅ΠΆΠΈΠΌΠ΅ ΠΠ¦Π Β«Π² ΡΠ΅Π°Π»ΡΠ½ΠΎΠΌ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈΒ» ΠΎΠΊΠ°Π·Π°Π»ΡΡ Π±ΠΎΠ»Π΅Π΅ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠ²Π½ΡΠΌ, ΡΠ΅ΠΌ ΠΏΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠΈ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΠ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½Π° ΡΠ°ΡΡΠ΅Π½ΠΈΡ-Ρ
ΠΎΠ·ΡΠΈΠ½Π°
ΠΠΈΠ΄ΠΎΠ²ΠΎΠ΅ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·ΠΈΠ΅ ΡΠΈΡΠΎΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΡΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΡΠΎΠ΄Π° Xanthomonas, ΠΏΠΎΡΠ°ΠΆΠ°ΡΡΠΈΡ ΡΠ°ΡΡΠ΅Π½ΠΈΡ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΌΡΡΠ»ΠΈΠΊΠΎΠ²ΡΠ΅ Π² Π ΠΎΡΡΠΈΠΈ
We reviewed the published data on a composition of plant pathogenic bacteria of the genus Xanthomonas damaging plant of family Poaceae (cereals) in Russia together with our new data obtained from surveys of cereal seeds from different regions of Russia. We assayed the xanthomonads on cereal seeds from different regions and have identified for the first time pathogens similar to species X. hortorum, X. campestris, X. cynarae, X. pisi, and X. gardeneri, which have not been reported yet on the cereal crops
ΠΠΈΠ΄ΠΎΠ²ΠΎΠ΅ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·ΠΈΠ΅ ΡΠΈΡΠΎΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΡΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΡΠΎΠ΄Π° Xanthomonas, ΠΏΠΎΡΠ°ΠΆΠ°ΡΡΠΈΡ ΡΠ°ΡΡΠ΅Π½ΠΈΡ ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΠΌΡΡΠ»ΠΈΠΊΠΎΠ²ΡΠ΅ Π² Π ΠΎΡΡΠΈΠΈ
We reviewed the published data on a composition of plant pathogenic bacteria of the genus Xanthomonas damaging plant of family Poaceae (cereals) in Russia together with our new data obtained from surveys of cereal seeds from different regions of Russia. We assayed the xanthomonads on cereal seeds from different regions and have identified for the first time pathogens similar to species X. hortorum, X. campestris, X. cynarae, X. pisi, and X. gardeneri, which have not been reported yet on the cereal crops
First report of Agrobacterium vitis causing crown galls of wine grape in Russia
Symptoms of crown gall were observed in 9 of 15 surveyed vineyards located along Black Sea coast of Russia. Sampled tumor tissue was placed in a mortar and pestle for maceration. Serial dilutions of the resulting suspension were plated onto RS medium described by Roy and Sasser (1983). Isolation plates were incubated for 5 days at 28Β°C until bacteria developed. Colonies were consistent with morphology expected of Agrobacterium spp. On RS medium (opaque red center, translucent margin, mucoid) were purified on yeast dextrose calcium carbonate agar (YDC). Sixty-nine putative Agrobacterium isolates were confirmed by PCR with consensus primers virD2A/2C from the virD2 gene (Haas et al. 1995). Isolatesβidentified by PCR and producing tumors on indicator plants (carrot, red beet, sunflower) and on grapevine plants in 30 days after needle prick inoculationβwere subjected to additional biochemical and physiological tests for Agrobacterium spp. (Moore et al. 2001). The tests included evaluation of 3-ketolactose production, alkaline reaction in litmus milk, growth on 2% and 5% NaCl, growth at 36Β°C, acid production from erythritol and melezitose, and alkali production from malonic acid and l-tartaric acid. Bacteria reisolated from inoculated grapevine plants were similar to original isolates in PCR test and 3-ketolactose production. The PGF/PGR primers amplifying the chromosomal polygalacturonase gene pehA (Szegedi and Bottka 2002) were used to identify A. vitis isolates and differentiate them from A. tumefaciens. Based on PCR, 18 of 69 tested isolates belonged to A. vitis and showed results of biochemical tests consisted with this species. In addition, for nine isolates, DNA sequence analysis of the housekeeping genes dnaK and trpE confirmed the isolates as A. vitis (Aujoulat et al. 2011). Sequences were deposited in GenBank as Accessions Nos. KT831413 to KT831421 for the dnaK gene and KT831422 to KT831430 for the trpE gene. Sequences were compared with corresponding genes of sequenced strain Agrobacterium vitis S4 (Accession No. CP000633.1). BLAST analysis revealed 99% homology for dnaK and 100% homology for trpE gene. This is the first documented Russian record of Agrobacterium vitis. Β© 2016, American Phytopathological Society. All rights reserved
First report of Agrobacterium vitis causing crown galls of wine grape in Russia
Symptoms of crown gall were observed in 9 of 15 surveyed vineyards located along Black Sea coast of Russia. Sampled tumor tissue was placed in a mortar and pestle for maceration. Serial dilutions of the resulting suspension were plated onto RS medium described by Roy and Sasser (1983). Isolation plates were incubated for 5 days at 28Β°C until bacteria developed. Colonies were consistent with morphology expected of Agrobacterium spp. On RS medium (opaque red center, translucent margin, mucoid) were purified on yeast dextrose calcium carbonate agar (YDC). Sixty-nine putative Agrobacterium isolates were confirmed by PCR with consensus primers virD2A/2C from the virD2 gene (Haas et al. 1995). Isolatesβidentified by PCR and producing tumors on indicator plants (carrot, red beet, sunflower) and on grapevine plants in 30 days after needle prick inoculationβwere subjected to additional biochemical and physiological tests for Agrobacterium spp. (Moore et al. 2001). The tests included evaluation of 3-ketolactose production, alkaline reaction in litmus milk, growth on 2% and 5% NaCl, growth at 36Β°C, acid production from erythritol and melezitose, and alkali production from malonic acid and l-tartaric acid. Bacteria reisolated from inoculated grapevine plants were similar to original isolates in PCR test and 3-ketolactose production. The PGF/PGR primers amplifying the chromosomal polygalacturonase gene pehA (Szegedi and Bottka 2002) were used to identify A. vitis isolates and differentiate them from A. tumefaciens. Based on PCR, 18 of 69 tested isolates belonged to A. vitis and showed results of biochemical tests consisted with this species. In addition, for nine isolates, DNA sequence analysis of the housekeeping genes dnaK and trpE confirmed the isolates as A. vitis (Aujoulat et al. 2011). Sequences were deposited in GenBank as Accessions Nos. KT831413 to KT831421 for the dnaK gene and KT831422 to KT831430 for the trpE gene. Sequences were compared with corresponding genes of sequenced strain Agrobacterium vitis S4 (Accession No. CP000633.1). BLAST analysis revealed 99% homology for dnaK and 100% homology for trpE gene. This is the first documented Russian record of Agrobacterium vitis. Β© 2016, American Phytopathological Society. All rights reserved