582 research outputs found
Efficient single photon emission from a high-purity hexagonal boron nitride crystal
Among a variety of layered materials used as building blocks in van der Waals
heterostructures, hexagonal boron nitride (hBN) appears as an ideal platform
for hosting optically-active defects owing to its large bandgap ( eV).
Here we study the optical response of a high-purity hBN crystal under green
laser illumination. By means of photon correlation measurements, we identify
individual defects emitting a highly photostable fluorescence under ambient
conditions. A detailed analysis of the photophysical properties reveals a high
quantum efficiency of the radiative transition, leading to a single photon
source with very high brightness. These results illustrate how the wide range
of applications offered by hBN could be further extended to photonic-based
quantum information science and metrology.Comment: 5 pages, 4 figure
Moderate hypothermia within 6 h of birth plus inhaled xenon versus moderate hypothermia alone after birth asphyxia (TOBY-Xe): a proof-of-concept, open-label, randomised controlled trial
Background Moderate cooling after birth asphyxia is associated with substantial reductions in death and disability, but additional therapies might provide further benefit. We assessed whether the addition of xenon gas, a promising novel therapy, after the initiation of hypothermia for birth asphyxia would result in further improvement. Methods Total Body hypothermia plus Xenon (TOBY-Xe) was a proof-of-concept, randomised, open-label, parallel-group trial done at four intensive-care neonatal units in the UK. Eligible infants were 36–43 weeks of gestational age, had signs of moderate to severe encephalopathy and moderately or severely abnormal background activity for at least 30 min or seizures as shown by amplitude-integrated EEG (aEEG), and had one of the following: Apgar score of 5 or less 10 min after birth, continued need for resuscitation 10 min after birth, or acidosis within 1 h of birth. Participants were allocated in a 1:1 ratio by use of a secure web-based computer-generated randomisation sequence within 12 h of birth to cooling to a rectal temperature of 33·5°C for 72 h (standard treatment) or to cooling in combination with 30% inhaled xenon for 24 h started immediately after randomisation. The primary outcomes were reduction in lactate to N-acetyl aspartate ratio in the thalamus and in preserved fractional anisotropy in the posterior limb of the internal capsule, measured with magnetic resonance spectroscopy and MRI, respectively, within 15 days of birth. The investigator assessing these outcomes was masked to allocation. Analysis was by intention to treat. This trial is registered with ClinicalTrials.gov, number NCT00934700, and with ISRCTN, as ISRCTN08886155. Findings The study was done from Jan 31, 2012, to Sept 30, 2014. We enrolled 92 infants, 46 of whom were randomly assigned to cooling only and 46 to xenon plus cooling. 37 infants in the cooling only group and 41 in the cooling plus xenon group underwent magnetic resonance assessments and were included in the analysis of the primary outcomes. We noted no significant differences in lactate to N-acetyl aspartate ratio in the thalamus (geometric mean ratio 1·09, 95% CI 0·90 to 1·32) or fractional anisotropy (mean difference −0·01, 95% CI −0·03 to 0·02) in the posterior limb of the internal capsule between the two groups. Nine infants died in the cooling group and 11 in the xenon group. Two adverse events were reported in the xenon group: subcutaneous fat necrosis and transient desaturation during the MRI. No serious adverse events were recorded. Interpretation Administration of xenon within the delayed timeframe used in this trial is feasible and apparently safe, but is unlikely to enhance the neuroprotective effect of cooling after birth asphyxia
Sensing remote nuclear spins
Sensing single nuclear spins is a central challenge in magnetic resonance
based imaging techniques. Although different methods and especially diamond
defect based sensing and imaging techniques in principle have shown sufficient
sensitivity, signals from single nuclear spins are usually too weak to be
distinguished from background noise. Here, we present the detection and
identification of remote single C-13 nuclear spins embedded in nuclear spin
baths surrounding a single electron spins of a nitrogen-vacancy centre in
diamond. With dynamical decoupling control of the centre electron spin, the
weak magnetic field ~10 nT from a single nuclear spin located ~3 nm from the
centre with hyperfine coupling as weak as ~500 Hz is amplified and detected.
The quantum nature of the coupling is confirmed and precise position and the
vector components of the nuclear field are determined. Given the distance over
which nuclear magnetic fields can be detected the technique marks a firm step
towards imaging, detecting and controlling nuclear spin species external to the
diamond sensor
Electron spin resonance of nitrogen-vacancy centers in optically trapped nanodiamonds
Using an optical tweezers apparatus, we demonstrate three-dimensional control
of nanodiamonds in solution with simultaneous readout of ground-state
electron-spin resonance (ESR) transitions in an ensemble of diamond
nitrogen-vacancy (NV) color centers. Despite the motion and random orientation
of NV centers suspended in the optical trap, we observe distinct peaks in the
measured ESR spectra qualitatively similar to the same measurement in bulk.
Accounting for the random dynamics, we model the ESR spectra observed in an
externally applied magnetic field to enable d.c. magnetometry in solution. We
estimate the d.c. magnetic field sensitivity based on variations in ESR line
shapes to be ~50 microTesla/Hz^1/2. This technique may provide a pathway for
spin-based magnetic, electric, and thermal sensing in fluidic environments and
biophysical systems inaccessible to existing scanning probe techniques.Comment: 29 pages, 13 figures for manuscript and supporting informatio
Properties of nitrogen-vacancy centers in diamond: group theoretic approach
We present a procedure that makes use of group theory to analyze and predict
the main properties of the negatively charged nitrogen-vacancy (NV) center in
diamond. We focus on the relatively low temperatures limit where both the
spin-spin and spin-orbit effects are important to consider. We demonstrate that
group theory may be used to clarify several aspects of the NV structure, such
as ordering of the singlets in the () electronic configuration, the
spin-spin and the spin-orbit interactions in the () electronic
configuration. We also discuss how the optical selection rules and the response
of the center to electric field can be used for spin-photon entanglement
schemes. Our general formalism is applicable to a broad class of local defects
in solids. The present results have important implications for applications in
quantum information science and nanomagnetometry.Comment: 30 pages, 6 figure
Continuous mode cooling and phonon routers for phononic quantum networks
We study the implementation of quantum state transfer protocols in phonon
networks, where in analogy to optical networks, quantum information is
transmitted through propagating phonons in extended mechanical resonator arrays
or phonon waveguides. We describe how the problem of a non-vanishing thermal
occupation of the phononic quantum channel can be overcome by implementing
optomechanical multi- and continuous mode cooling schemes to create a 'cold'
frequency window for transmitting quantum states. In addition, we discuss the
implementation of phonon circulators and switchable phonon routers, which rely
on strong coherent optomechanical interactions only, and do not require strong
magnetic fields or specific materials. Both techniques can be applied and
adapted to various physical implementations, where phonons coupled to spin or
charge based qubits are used for on-chip networking applications.Comment: 33 pages, 8 figures. Final version, a few minor changes and updated
reference
Monitoring Ion Channel Function In Real Time Through Quantum Decoherence
In drug discovery research there is a clear and urgent need for non-invasive
detection of cell membrane ion channel operation with wide-field capability.
Existing techniques are generally invasive, require specialized nano
structures, or are only applicable to certain ion channel species. We show that
quantum nanotechnology has enormous potential to provide a novel solution to
this problem. The nitrogen-vacancy (NV) centre in nano-diamond is currently of
great interest as a novel single atom quantum probe for nanoscale processes.
However, until now, beyond the use of diamond nanocrystals as fluorescence
markers, nothing was known about the quantum behaviour of a NV probe in the
complex room temperature extra-cellular environment. For the first time we
explore in detail the quantum dynamics of a NV probe in proximity to the ion
channel, lipid bilayer and surrounding aqueous environment. Our theoretical
results indicate that real-time detection of ion channel operation at
millisecond resolution is possible by directly monitoring the quantum
decoherence of the NV probe. With the potential to scan and scale-up to an
array-based system this conclusion may have wide ranging implications for
nanoscale biology and drug discovery.Comment: 7 pages, 6 figure
Characterising a human endogenous retrovirus(HERV)-derived tumour-associated antigen: enriched RNA-Seq analysis of HERV-K(HML-2) in mantle cell lymphoma cell lines.
BACKGROUND: The cell-surface attachment protein (Env) of the HERV-K(HML-2) lineage of endogenous retroviruses is a potentially attractive tumour-associated antigen for anti-cancer immunotherapy. The human genome contains around 100 integrated copies (called proviruses or loci) of the HERV-K(HML-2) virus and we argue that it is important for therapy development to know which and how many of these contribute to protein expression, and how this varies across tissues. We measured relative provirus expression in HERV-K(HML-2), using enriched RNA-Seq analysis with both short- and long-read sequencing, in three Mantle Cell Lymphoma cell lines (JVM2, Granta519 and REC1). We also confirmed expression of the Env protein in two of our cell lines using Western blotting, and analysed provirus expression data from all other relevant published studies. RESULTS: Firstly, in both our and other reanalysed studies, approximately 10% of the transcripts mapping to HERV-K(HML-2) came from Env-encoding proviruses. Secondly, in one cell line the majority of the protein expression appears to come from one provirus (12q14.1). Thirdly, we find a strong tissue-specific pattern of provirus expression. CONCLUSIONS: A possible dependency of Env expression on a single provirus, combined with the earlier observation that this provirus is not present in all individuals and a general pattern of tissue-specific expression among proviruses, has serious implications for future HERV-K(HML-2)-targeted immunotherapy. Further research into HERV-K(HML-2) as a possible tumour-associated antigen in blood cancers requires a more targeted, proteome-based, screening protocol that will consider these polymorphisms within HERV-K(HML-2). We include a plan (and necessary alignments) for such work
Observation of anomalous decoherence effect in a quantum bath at room temperature
Decoherence of quantum objects is critical to modern quantum sciences and
technologies. It is generally believed that stronger noises cause faster
decoherence. Strikingly, recent theoretical research discovers the opposite
case for spins in quantum baths. Here we report experimental observation of the
anomalous decoherence effect for the electron spin-1 of a nitrogen-vacancy
centre in high-purity diamond at room temperature. We demonstrate that under
dynamical decoupling, the double-transition can have longer coherence time than
the single-transition, even though the former couples to the nuclear spin bath
as twice strongly as the latter does. The excellent agreement between the
experimental and the theoretical results confirms the controllability of the
weakly coupled nuclear spins in the bath, which is useful in quantum
information processing and quantum metrology.Comment: 22 pages, related paper at http://arxiv.org/abs/1102.557
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