On the Bio-Rearrangement into Fully Saturated Fatty Acids-Containing Triglyceride in Aurantiochytrium sp

AbstractA strain of Aurantiochytrium sp. was grown in media with various concentrations of glucose to monitor triglyceride production as a potential source of oil for biodiesel. The fatty acid composition of triglyceride in the strain was unique, because the fatty acids consisted of only 6 molecular species, and the major species were myristic, pentadecanoic, palmitic, heptadecanoic, docosapentaenoic, and docosahexaenoic acids. When cells were cultured in glucose-rich (over 9%) medium for 4 days, the triglyceride yields were 0.5-1.0g/L. After culture for 4 days, the fatty acid composition of triglyceride was nearly identical in all cells grown in media containing various concentrations of glucose. However, when cells were grown in medium containing 12% glucose for 12 days, unique triglyceride containing only saturated fatty acids accumulated. This bio-rearrangement into fully-saturated fatty acids-containing triglyceride may be utilized for the preparation of biodiesel oil

高校生物2における自然環境に関する課題研究の指導法の開発

本研究では、生物2の分野における「自然環境についての課題研究」の単元において、生徒たちが身のまわりの自然環境に対して興味や疑問を持ち、生物学的に探求する態度や方法を身につけることを目的とした、計6時間の学習指導案を立案した。本研究で立案した学習指導案は、以下の点において新規性・有用性があると考えられる。1．生物2「自然環境についての課題研究」において、一単元の「教師用の指導用ガイド」を作成した。2．自然環境の「土壌」を測定する手法を開発し、身近な材料を用いた測定ツールを製作した。3．調査対象に「土壌」を取り上げることで、より身近な環境に目を向けさせるとともに、生徒自身で取り組むことができる、より簡便な採取作業・調査方法を提示した。In the present study, educational guidance of six hours was developed to induce an interest in the natural environment around them and acquire techniques and attitudes for exploring biological issues as part of "Theme study on natural environment", in high school Biology 2 class. The educational guidance examined in the present study is novel and significant in the following ways:1. In Biology 2, "Theme study on natural environment", one-unit "Instructional guide for teachers" was developed.2. A technique for measuring soil in the natural environment was developed and measurement tools were prepared using familiar materials.3. By focusing on soil as a research objective, more convenient sampling and investigational methods were developed to allow students to examine familiar environments on their own

PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast

PCNA ubiquitylation on lysine 164 is required for DNA damage tolerance. In many organisms PCNA is also ubiquitylated in unchallenged S phase but the significance of this has not been established. Using Schizosaccharomyces pombe, we demonstrate that lysine 164 ubiquitylation of PCNA contributes to efficient DNA replication in the absence of DNA damage. Loss of PCNA ubiquitylation manifests most strongly at late replicating regions and increases the frequency of replication gaps. We show that PCNA ubiquitylation increases the proportion of chromatin associated PCNA and the co-immunoprecipitation of Polymerase δ with PCNA during unperturbed replication and propose that ubiquitylation acts to prolong the chromatin association of these replication proteins to allow the efficient completion of Okazaki fragment synthesis by mediating gap filling

Accuracy of breathing and radial pulse assessment by non-medical persons: an observational cross-sectional study

Abstract Early recognition of cardiopulmonary arrest (CPA) expedites emergency calls and resuscitation and improves the survival rate of unresponsive individuals. However, the accuracy of breathing and radial artery pulse assessment by non-medical persons is poorly understood. The aim of this study was to determine the accuracy of breathing assessment and radial pulse palpation among 450 non-medical personnel using a high-fidelity simulator. We examined the accuracy of 10 second’s assessment for breathing and radial pulse using a high-fidelity mannequin simulator, included 496 non-medical participants (school teachers) between 2016–2018. For a primary results, the sensitivity for the detection of the presence of the breathing and radial pulse was 96.2% (97.5% for sensitivity and 92.0% for specificity) and 91.7% (99.1% for sensitivity and 56.8% for specificity), respectively. Futher, breathing rate and radial pulse rate were strongly correlated with the assessments, with Spearman’s correlation coefficients of 0.813 (P < 0.001) and 0.719 (P < 0.001), respectively. In contrast, radial pulse strength was weakly correlated with the assessment (coefficient of 0.288, P < 0.001). Our results suggested that non-medical persons would show high accuracy in detecting and measuring respiration and radial pulse, although they did not accurately determine radial pulse strength for the early recognition of CPA

Electrical Conductivity as an Indicator to Assess the Suitability of River Water for Recreational Use

Urban rivers flowing through cities are places of recreation and relaxation for citizens. However, these rivers are sometimes contaminated by Escherichia coli (E. coli). Therefore, the purpose of this study was to develop a simple method to investigate E. coli contamination in river water. From May to October 2019, water samples were collected from five locations in the Toga River in Kobe City, Japan, and the fecal coliform density (FCD) was measured, along with the electrical conductivity and the chloride ion concentration of the river water. Comparison of these water quality parameters with actual fecal coliform densities revealed a high correlation between electrical conductivity and FCD. Whereas, little correlation was found between FCD and chloride concentration. Receiver operating characteristic (ROC) analysis was used to evaluate the method that uses the electrical conductivity as an estimating parameter. The area under the curve (AUC) was used as a measure of the performance of the ROC curve algorithm. The calculated AUC value stayed high, above 0.95, over a wide range of FCD values, suggesting that this rapid monitoring method is appropriate for assessing the quantity of contaminating fecal coliforms in the range higher than 300/100 mL

Prenatal PFAAs exposure and IGF2/H19 methylation

Prenatal exposure to perfluoroalkyl acids (PFAAs) influences fetal growth and long-term health. However, whether PFAAs affect offspring DNA methylation patterns to influence health outcomes is yet to be evaluated. Here, we assessed effect of prenatal PFAA exposure on cord blood insulin-like growth factor 2 (IGF2), H19, and long interspersed element 1 (LINE1) methylation and its associations with birth size. Mother-child pairs (N=177) from the Hokkaido Study on Environment and Children's Health were included in the study. Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) levels in maternal serum were measured by liquid chromatography-tandem mass spectrometry. IGF2, H19, and LINE1 methylation in cord blood DNA was determined by pyrosequencing. After full adjustment in multiple linear regression models, IGF2 methylation showed a significant negative association with log-unit increase in PFOA (partial regression coefficient=-0.73; 95% confidence interval: -1.44 to -0.02). Mediation analysis suggested that reduced IGF2 methylation explained approximately 21% of the observed association between PFOA exposure and reduced ponderal index of the infant at birth. These results indicated that the effects of prenatal PFOA exposure could be mediated through DNA methylation. Further study will be required to determine the potential for long-term adverse health effects of reduced IGF2 methylation induced by PFOA exposure