A Study of Natural Radioactivity Levels and Radon/Thoron Release Potential of Bedrock and Soil in Southeastern Ireland

Radon (222Rn) and thoron (220Rn) account for almost two-thirds of the annual average radiation dose received by the Irish population. A detailed study of natural radioactivity levels and radon and thoron exhalation rates was carried out in a legislatively designated “high radon” area, as based on existing indoor radon measurements. Indoor radon concentrations, airborne radio-metric data and stream sediment geochemistry were collated, and a set of soil samples were taken from the study area. The exhalation rates of radon (E222Rn) and thoron (E220Rn) for collected samples were determined in the laboratory. The resultant data were classified based on geological and soil type parameters. Geological boundaries were found to be robust classifiers for radon exhalation rates and radon-related variables, whilst soil type classification better differentiates thoron ex-halation rates and correlated variables. Linear models were developed to predict the radon and thoron exhalation rates of the study area. Distribution maps of radon and thoron exhalation rates (range: E222Rn [0.15-1.84] and E220Rn [474.69-3028.85] Bq m−2 h−1) and annual effective dose (with a mean value of 0.84 mSv y-1) are presented. For some parts of the study area, the calculated annual effective dose exceeds the recommended level of 1 mSv y-1, illustrating a significant radiation risk. Airborne radiometric data were found to be a powerful and fast tool for the prediction of geogenic radon and thoron risk. This robust method can be used for other areas where airborne radiometric data are available

A short G1 phase is an intrinsic determinant of naïve embryonic stem cell pluripotency

AbstractA short G1 phase is a characteristic feature of mouse embryonic stem cells (ESCs). To determine if there is a causal relationship between G1 phase restriction and pluripotency, we made use of the Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) reporter system to FACS-sort ESCs in the different cell cycle phases. Hence, the G1 phase cells appeared to be more susceptible to differentiation, particularly when ESCs self-renewed in the naïve state of pluripotency. Transitions from ground to naïve, then from naïve to primed states of pluripotency were associated with increased durations of the G1 phase, and cyclin E-mediated alteration of the G1/S transition altered the balance between self-renewal and differentiation. LIF withdrawal resulted in a lengthening of the G1 phase in naïve ESCs, which occurred prior to the appearance of early lineage-specific markers, and could be reversed upon LIF supplementation. We concluded that the short G1 phase observed in murine ESCs was a determinant of naïve pluripotency and was partially under the control of LIF signaling

Stress path during pressuremeter test and link between shear modulus and Menard pressuremeter modulus in unsaturated fine soils

This paper presents the stress path before and during a pressumeter test using a Modified Cam-Clay model. This model have been used for unsaturated clay with continuous water phase (D2 domain according to Boutonnier 2007) and for silty to clayey soils with discontinuous water phase (D1 domain – ibid.). After reminding the stress distribution around a pressuremeter cell and the meaning of the Menard pressuremeter modulus which is linked to the soil shear modulus, we explain the stress state during pressuremeter modulus measurement. Then, we show that this stress path diagram can be used to explain classical observations made using Menard pressuremeter tests in continental area and typically: Measurement of high ratio “Menard pressuremeter modulus / pressuremeter limit pressure” in clay subjected to drought

Stress path during pressuremeter test and link between shear modulus and Menard pressuremeter modulus in unsaturated fine soils

This paper presents the stress path before and during a pressumeter test using a Modified Cam-Clay model. This model have been used for unsaturated clay with continuous water phase (D2 domain according to Boutonnier 2007) and for silty to clayey soils with discontinuous water phase (D1 domain – ibid.). After reminding the stress distribution around a pressuremeter cell and the meaning of the Menard pressuremeter modulus which is linked to the soil shear modulus, we explain the stress state during pressuremeter modulus measurement. Then, we show that this stress path diagram can be used to explain classical observations made using Menard pressuremeter tests in continental area and typically: Measurement of high ratio “Menard pressuremeter modulus / pressuremeter limit pressure” in clay subjected to drought. Limit pressure and Ménard pressuremeter modulus variation with respect to drought in silty clay situated in water-table fluctuation area. Finally, we will try to show the consequences of these stress paths for performing and interpreting pressuremeter test in unsaturated and collapsible soils

Krüppel like factors reprogram rabbit induced pluripotent stem cells to naïve like pluripotency

International audienc

Transcriptional reconfiguration of rabbit iPS cells with Krüppel-like factors confers chimeric competency

National audienc

Transcriptional reconfiguration of rabbit iPS cells with Krüppel-like factors confers chimeric competency

National audienc

A Panel of Embryonic Stem Cell Lines Reveals the Variety and Dynamic of Pluripotent States in Rabbits

Conventional rabbit embryonic stem cell (ESC) lines are derived from the inner cell mass (ICM) of pre-implantation embryos using methods and culture conditions that are established for primate ESCs. In this study, we explored the capacity of the rabbit ICM to give rise to ESC lines using conditions similar to those utilized to generate naive ESCs in mice. On single-cell dissociation and culture in fibroblast growth factor 2 (FGF2)-free, serum-supplemented medium, rabbit ICMs gave rise to ESC lines lacking the DNA-damage checkpoint in the G1 phase like mouse ESCs, and with a pluripotency gene expression profile closer to the rabbit ICM/epiblast profiles. These cell lines can be converted to FGF2-dependent ESCs after culture in conventional conditions. They can also colonize the rabbit pre-implantation embryo. These results indicate that rabbit epiblast cells can be coaxed toward different types of pluripotent stem cells and reveal the dynamics of pluripotent states in rabbit ESCs

Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells

Kruppel-like factors (Klf) 4 and 5 are two closely related members of the Klf family, known to play key roles in cell cycle regulation, somatic cell reprogramming and pluripotency. Here we focus on the functional divergence between Klf4 and Klf5 in the inhibition of mouse embryonic stem (ES) cell differentiation. Using microarrays and chromatin immunoprecipitation coupled to ultra-high-throughput DNA sequencing, we show that Klf4 negatively regulates the expression of endodermal markers in the undifferentiated ES cells, including transcription factors involved in the commitment of pluripotent stem cells to endoderm differentiation. Knockdown of Klf4 enhances differentiation towards visceral and definitive endoderm. In contrast, Klf5 negatively regulates the expression of mesodermal markers, some of which control commitment to the mesoderm lineage, and knockdown of Klf5 specifically enhances differentiation towards mesoderm. We conclude that Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in murine ES cells