Cell Proteomic Footprint

The authentication of mammalian cell cultures and their subpopulations are of tremendous demand in biotechnology and cell therapy. However, current techniques are either not efficient or can be very complex and expensive. Here we report a simple and straightforward approach for authentication of biological cells and their subpopulations with high speed, high throughput, low sample cost, and high sensitivity. We discovered that cell cultures treated with protease at soft, “non-killing” conditions release fragments of cell surface proteins, which composition is a strong characteristic of the cells. Mass spectrometric analysis of the released fragments allows a direct comparison of the produced mass spectrum with the mass spectrum of known cells. As an example, we applied this technique to verify subpopulations of human fibroblasts which have different origins and exhibit different medical characteristics

Potential of regional fuel and energy complex with the typological features of regional development

Purpose: Fuel & Energy Complex (FEC) has a significant share in the economic system and has the most extensive potential for introducing innovations. Authors suggest innovative potential of regional FEC considering the specifics of regional economic development. Design/Methodology/Approach: To evaluate the attainability of economic growth in conjunction with the goals of energy efficiency and energy independence and to make decisions on adjustments in energy policy, the authors propose a three-component integrated index of elasticity of energy efficiency for each typological group of region, including the index of innovative potential of the regional Fuel & Energy Complex, the index of energy supply efficiency of consumers in the region and the sustainability index of the region’s energy system. Findings: The survey identified five typological groups of regions reflecting the features of regional FEC development. Considering the significance of factors affecting innovative potential, seven groups of such factors were proposed for formation, and the significance of each factor was determined in accordance with the typological group of the region. Practical implications: Each of the three components of the integrated index makes its specific contribution to the resulting quantitative indicator, the value of which will reflect the degree to which the regional energy policy has reached its target priorities, and to meet the urgent challenges of modern innovative sustainable energy technologies. Originality/Value: Realisation of the innovative potential of regional FEC should be closely linked with indicators of energy efficiency and energy independence in order to ensure the development of regional economies, including the achievement of global climate goals.peer-reviewe

EVALUATION OF INTELLECTUAL CAPITAL IN PRODUCTION

In the article was elucidated the essence of intellectual capital and its role in productive activity. At establishing the knowledge-based model of economy the formation of intellectual industrial property as one of the main components of intellectual capital of existing productions needs planning, clear organization and motivation of this process. The author defined an attempt to evaluate an effectiveness of using intellectual capital as the problem of research. The categorical essence of intellectual capital was analyzed from the positions of the different authors. There was offered the author’s classification of intellectual capital content where it is divided into internal and external structure and also in the staff competence. At the same time there is the certain correspondence between the structural components of intellectual capital in both variants of their singling out. There was offered the system of interconnection between the objects of intellectual capital and technological processes or means of technological equipping applied to the subdivisions of the main and auxiliary productions. It was grounded, that the management of intellectual capital needs planning, clear organization and motivation of this process through the analysis of costs connected with its creation. There was offered the quantitative assessment of the effectiveness of intellectual capital use. There was established the value of this parameter as the reference point for attainment of the effective innovative development of industrial enterprises

DYNAMICS OF SOME PHYSICAL AND CHEMICAL INDICATORS OF SUCEAVA RIVER WATER IN 2009

For a physical-chemical evaluation of global quality of Suceava river water, water samples were collected every two months during 2009, on the following sections (sites, locations): • Brodina at 142 km distance from mouth; • Mihoveni, 45 km distance from mouth; • Tişăuţi, 34 km distance from mouth. The values obtained from physical - chemical analyzes of water were processed and compared with the limit values of quality grades, provided by the norm on surface water quality classification, in order to determine the ecological status of water bodies according to Order nr.161/2006. Along Suceava river, the water flows increase from upstream to downstream: the maximum value 4.47 m3/s at Brodina (14.07.2009) increase at Mihoveni, the maximum value being 13.22 m3/s (14.07.2009) and at Tişăuţi, the flow recorded on 14.07.2009 is 13.7 m3/s. The annual average flows have fluctuated on very wide limits, depending on pluviometrical characteristics of rainfall on each year. Usually, the highest average flow is recorded in April, followed by June (Maxim, 2012). Higher levels of flow in these months can be explained by strong contribution of rainwater supply to which is added the water from snowmelt. The smallest amounts of water are flowing in winter and autumn. This paper systematizes the results concerning the dynamics of some experimentally determined physical and chemical indicators during 2009, on Suceava river water. (PH, OD, CBO5, CCOMn, CCOCr).In order to have an accurate picture of analyzed physico-chemical parameters, the average values of these indicators were presented for comparison. Thus, the concentration of hydrogen ions (pH) has values between 8 in the lower course and 8.3 at the upper course, at Brodina. At this value, the waters are favourable for the organisms development. The medium content of dissolved oxygen decreases from February to July and then increase until December - January, the average values ranging between 10.53 mg/l and 14.25 mg/l. Biochemical oxygen demand has an average value of 1.45 mg/l in the upper course and 6.25 mg/l at the mouth. The chemical oxygen demand falls between 2.34 mg/l at Brodina and 4.67 mg/l at mouth, on Suceava. In 2009, Suceava River in the control sections Brodina, Mihoveni and Tişăuţi was framed in quality classes I and II for all analyzed indicators