5 research outputs found
Detection of Blueberry red ringspot virus in highbush blueberry cv. ‘Coville’ in Slovenia
Blueberry red ringspot virus (BRRSV) infects blueberries and is present in USA. It causes red ringspots or red blotches on one year old stems or older. In mid- to late summer reddish-brown spots develop on older leaves. In some blueberry cultivars also fruit symptoms, circular areas of light colour and/or fruit deformations, can be seen. In spring 2008 BRRSV was detected in symptomatic bark from blueberry cv. ‘Coville’ showing typical BRRSV symptoms. The obtained PCR product was sequenced and the identity of the virus confirmed. To our knowledge this was the first finding of BRRSV in Slovenia.Keywords: BRRSV, Vaccinium, PCR, detectio
Comparison of Raspberry bushy dwarf virus isolates from Hungary and Slovenia
In 2006 and 2007 samples of grapevine and Rubus species were collected and analysed by DAS-ELISA to survey the presence of Raspberry bushy dwarf virus (RBDV) in Slovenia and Hungary. Seven varieties of raspberry from one Hungarian collection orchard were found to be infected. In Slovenia the presence of RBDV was confirmed only in three samples of wild Rubus. None of the 133 samples from different locations in Hungary proved to be infected with RBDV, although this virus is found to be widely distributed in grapevine in neighbouring Slovenia. Serological characterisation with three monoclonal antibodies (R2, R5 and D1) was performed on positive samples. Selected positive samples were partially sequenced. The results of serological and molecular analyses were compared with the analyses of raspberry and grapevine isolates obtained in Slovenia from other projects and published RBDV sequences from the GeneBank database to study the variability among hosts and locations. Isolates from grapevine grouped separately from the black raspberry isolate and all the red raspberry isolates. RBDV isolates from Hungarian samples formed a subgroup within red and black raspberry group.Keywords: RBDV, variability, Rubus, raspberry, grapevine, sequences, monoclonal antibodie