Blood Pressure, Serum Glucose, Cholesterol, and Triglycerides in Dogs with Different Body Scores

The objective of this research was to determine the frequency for the occurrence of MS in dogs, using the criteria determined, and to correlate the criteria of dogs that would characterize the MS with different body condition score (BCS). 271 dogs with different body scores were studied, with 101 dogs with BCS 4-5; 101 dogs with BCS 6-7; and 69 dogs with BCS 8-9. Among the dogs studied, 62 (22,87%) had two or more inclusion criteria for MS. Of these, 28 had BCS 6-7, while 34 dogs had BCS 8-9. Therefore, 27,72% of overweight dogs had inclusion criteria for MS and 49,27% of obese ones had two or more inclusion criteria for MS. When only overweight and obese dogs were considered as a total population, it was observed that 36,47% got inclusion criteria for the MS. No dog with BCS 4-5 showed two or more inclusion criteria for MS. The metabolic syndrome, according to the parameters for inclusion defined in the literature, was observed in 22,87% of the animals studied and in 36% of dogs overweight or obese. Furthermore, MS was most common in obese (49%) compared to overweight dogs (27%)

Chronic alcohol administration alters metabolomic profile of murine bone marrow

IntroductionPeople with hazardous alcohol use are more susceptible to viral, bacterial, and fungal infections due to the effect of alcohol on immune system cell function. Metabolized ethanol reduces NAD+ to NADH, affecting critical metabolic pathways. Here, our aim was to investigate whether alcohol is metabolized by bone marrow cells and if it impacts the metabolic pathways of leukocyte progenitor cells. This is said to lead to a qualitative and quantitative alteration of key metabolites which may be related to the immune response.MethodsWe addressed this aim by using C57BL/6 mice under chronic ethanol administration and evaluating the metabolomic profile of bone marrow total cells by gas chromatography–coupled mass spectrometry (GC–MS).ResultsWe identified 19 metabolites. Our data demonstrated that chronic ethanol administration alters the metabolomic profile in the bone marrow, resulting in a statistically diminished abundance of five metabolites in ethanol-treated animals: uracil, succinate, proline, nicotinamide, and tyrosine.DiscussionOur results demonstrate for the first time in the literature the effects of alcohol consumption on the metabolome content of hematopoietic tissue and open a wide range of further studies to investigate mechanisms by which alcohol compromises the cellular function of the immune system

Etiological Factor of the Ossification of the Stylohyoid Ligament

ResumoObjetivo: avaliar o ligamento estilohióide sob tensão em função da posição anteriorizada da mandíbula, em paciente que apresentaram, exclusivamente, desordem temporomandibular (DTM) e que não houvesse coincidência da ralação cêntrica e máxima intercuspidação. Material e métodos: Participaram deste estudo 85 pacientes, sendo 65 do gênero feminino e 20 masculino, com idade que variou de 18 a 78 anos, tendo sido selecionados aqueles que apresentaram Desordem Temporomandibular (DTM), com relação central (RC) não coincidente com a máxima intercuspidação (MI) e que não tivessem sido submetidos a tonsilectomia ou cirurgia das carótidas. Para a coleta de dados foram utilizadas radiografias panorâmicas, cujos dados foram anotados em ficha apropriada. Resultado: O ligamento estilohióide pode apresentar alterações, entre as quais, está seu alongamento e sua ossificação, que foi uma anomalia bastante frequente. Conclusão: Os resultados mostraram que foi significativo o número de pacientes com os ligamentos estilohióides alongados e ossificados.Palavras-chave: Ligamento estilohióide; Desordem temporomandibular; Relação central..  AbstractObjective: The purpose of this study was to evaluate the hyoid ligament style under stress due to the forward position of the mandible in a patient who presented exclusively temporomandibular disorder (TMD) and that there was no coincidence of centric and maximum intercuspation grating. Methods: The study included 85 patients, 65 female and 20 male subjects, ages ranged from 18 to 78 years, having been selected those with temporomandibular disorder, with central relationship (RC) does not coincide with the maximum intercuspation (MI), which had not undergone tonsillectomy or surgery of the carotid arteries. For data collection panoramic radiographs were used, whose data were recorded in appropriate form. Result: The etylohiyode ligament may submit amendments, among which is its elongation and its ossification, which was a fairly frequent anomaly. Conclusion: The results showed that there was a significant number of patients with elongated stylohyoids and ossified ligaments.Keywords: Stylohyoid ligament; Temporomandibular disorder; Centric relation.Borges et al./ Sci Invest Dent 2016; 21(1):33-383

SELECTION OF Handroanthus impetiginosus MOTHER TREES TO SUPPORT SEED COLLECTION AREAS

ABSTRACT Handroanthus impetiginosus is a Brazilian native tree threatened by illegal logging that needs conservation strategies. Due to the need to obtain seeds of high genetic and physiological quality, this study aimed to select mother trees, aiming at the installation of Seed Collection Areas of H. impetiginosus. Therefore, the genetic diversity and physiological quality of seeds from 63 individuals of a natural population of H. impetiginosus in Rio Grande do Norte, Brazil, were evaluated. The molecular markers revealed that the studied population has high intrapopulation genetic diversity (Nei’s genetic diversity = 0.35; and Shannon index = 0.52). Subsequently, germination and seed vigor tests evaluated the physiological quality of seeds from 63 trees. Of this total, 45 individuals produced seeds of high and intermediate physiological quality. Therefore, individuals with a Nei’s genetic distance < 0.72 and germination greater than 50% can be selected as mother trees, and the population has the potential to propose a Seed Collection Area

Comprehensive Characterization of the Regulatory Landscape of Adrenocortical Carcinoma: Novel Transcription Factors and Targets Associated with Prognosis

We reconstructed a transcriptional regulatory network for adrenocortical carcinoma (ACC) using transcriptomic and clinical data from The Cancer Genome Atlas (TCGA)-ACC cohort. We investigated the association of transcriptional regulatory units (regulons) with overall survival, molecular phenotypes, and immune signatures. We annotated the ACC regulons with cancer hallmarks and assessed single sample regulon activities in the European Network for the Study of Adrenal Tumors (ENSAT) cohort. We found 369 regulons associated with overall survival and subdivided them into four clusters: RC1 and RC2, associated with good prognosis, and RC3 and RC4, associated with worse outcomes. The RC1 and RC3 regulons were highly correlated with the ‘Steroid Phenotype,’ while the RC2 and RC4 regulons were highly correlated with a molecular proliferation signature. We selected two regulons, NR5A1 (steroidogenic factor 1, SF-1) and CENPA (Centromeric Protein A), that were consistently associated with overall survival for further downstream analyses. The CENPA regulon was the primary regulator of MKI-67 (a marker of proliferation KI-67), while the NR5A1 regulon is a well-described transcription factor (TF) in ACC tumorigenesis. We also found that the ZBTB4 (Zinc finger and BTB domain-containing protein 4) regulon, which is negatively associated with CENPA in our transcriptional regulatory network, is also a druggable anti-tumorigenic TF. We anticipate that the ACC regulons may be used as a reference for further investigations concerning the complex molecular interactions in ACC tumors

ESTRATÉGIAS DE MARKETING VERDE EM REDES HOTELEIRAS

Com o aumento do turismo como forma de lazer, a indústria hoteleira passou a ser de grande importância para as questões ambientais e começou a adotar práticas verdes como diferencial. Este artigo tem por objetivo analisar como o marketing verde está sendo utilizado nas redes hoteleiras para atrair clientes, e como as mesmas impactam e influencia na tomada de decisão dos consumidores. As práticas hoteleiras podem ser adaptadas à escolha da região, tipos de clientes, missão e visão do hotel e o impacto que o mesmo quer ter sobre a comunidade que está inserido, como por exemplo com a escolha adequada dos materiais, tanto para a construção do edifício como para sua manutenção, reduzindo o consumo de energia e demais recursos básicos, treinamento de funcionários e serviços oferecidos aos cliente

Chronic ethanol exposure impairs alveolar leukocyte infiltration during pneumococcal pneumonia, leading to an increased bacterial burden despite increased CXCL1 and nitric oxide levels

Ethanol abuse is a risk factor for the development of pneumonia caused by Streptococcus pneumoniae, a critical pathogen for public health. The aim of this article was to investigate the inflammatory mechanisms involved in pneumococcal pneumonia that may be associated with chronic ethanol exposure. Male C57BL6/J-Unib mice were exposed to 20% (v/v) ethanol for twelve weeks and intranasally infected with 5x104 CFU of S. pneumoniae. Twenty-four hours after infection, lungs, bronchoalveolar lavage and blood samples were obtained to assess the consequences of chronic ethanol exposure during infection. Alcohol-fed mice showed increased production of nitric oxide and CXCL1 in alveoli and plasma during pneumococcal pneumonia. Beside this, ethanol-treated mice exhibited a decrease in leukocyte infiltration into the alveoli and reduced frequency of severe lung inflammation, which was associated with an increase in bacterial load. Curiously, no changes were observed in survival after infection. Taken together, these results demonstrate that chronic ethanol exposure alters the inflammatory response during S. pneumoniae lung infection in mice with a reduction in the inflammatory infiltrate even in the presence of higher levels of the chemoattractant CXCL1

Glucocorticoid-Induced Leucine Zipper Alleviates Lung Inflammation and Enhances Bacterial Clearance during Pneumococcal Pneumonia

Pneumonia is a leading cause of morbidity and mortality. While inflammation is a host protective response that ensures bacterial clearance, a finely regulated response is necessary to prevent bystander tissue damage. Glucocorticoid (GC)-induced leucine zipper (GILZ) is a GC-induced protein with anti-inflammatory and proresolving bioactions, yet the therapeutical role of GILZ in infectious diseases remains unexplored. Herein, we investigate the role and effects of GILZ during acute lung injury (ALI) induced by LPS and Streptococcus pneumoniae infection. GILZ deficient mice (GILZ−/−) presented more severe ALI, characterized by increased inflammation, decreased macrophage efferocytosis and pronounced lung damage. In contrast, pulmonary inflammation, and damage were attenuated in WT mice treated with TAT-GILZ fusion protein. During pneumococcal pneumonia, TAT-GILZ reduced neutrophilic inflammation and prevented the associated lung damage. There was also enhanced macrophage efferocytosis and bacterial clearance in TAT-GILZ-treated mice. Mechanistically, TAT-GILZ enhanced macrophage phagocytosis of pneumococcus, which was lower in GILZ−/− macrophages. Noteworthy, early treatment with TAT-GILZ rescued 30% of S. pneumoniae-infected mice from lethal pneumonia. Altogether, we present evidence that TAT-GILZ enhances host resilience and resistance to pneumococcal pneumonia by controlling pulmonary inflammation and bacterial loads leading to decreased lethality. Exploiting GILZ pathways holds promise for the treatment of severe respiratory infections