10 research outputs found

    Complete genome sequence of maize yellow striate virus, a new cytorhabdovirus infecting maize and wheat crops in Argentina

    No full text
    A rhabdovirus infecting maize and wheat crops in Argentina was molecularly characterized. Through next-generation sequencing (NGS) of symptomatic leaf samples, the complete genome was obtained of two isolates of maize yellow striate virus (MYSV), a putative new rhabdovirus, differing by only 0.4% at the nucleotide level. The MYSV genome consists of 12,654 nucleotides for maize and wheat virus isolates, and shares 71% nucleotide sequence identity with the complete genome of barley yellow striate mosaic virus (BYSMV, NC028244). Ten open reading frames (ORFs) were predicted in the MYSV genome from the antigenomic strand and were compared with their BYSMV counterparts. The highest amino acid sequence identity of the MYSV and BYSMV proteins was 80% between the L proteins, and the lowest was 37% between the proteins 4. Phylogenetic analysis suggested that the MYSV isolates are new members of the genus Cytorhabdovirus, family Rhabdoviridae. Yellow striate, affecting maize and wheat crops in Argentina, is an emergent disease that presents a potential economic risk for these widely distributed crops.Instituto de BiotecnologíaFil: Maurino, María Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dumon, Analia Delina Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Alemandri, Vanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gimenez Pecci, María de la Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentin

    First occurrence of a rhabdovirus infecting maize in Argentina

    No full text
    Maize (Zea mays) plants showing symptoms of shortened internodes, dwarfism, panicle sterility, and a mosaic of coarse and fine yellow stripes on leaf blades and sheaths, were found from December to March in experimental maize plantings in every crop year since 2000-01. Although the disease appeared at a very low incidence (estimates less than 1%), it was found in several locations such as Santa Isabel and Venado Tuerto, Santa Fe Province; Río Cuarto, Colonia Caroya, Río Segundo, and Sampacho, Córdoba Province; and Pedro Luro, Buenos Aires Province. Leaf tissue from eight symptomatic plants collected in Colonia Caroya in December 2011 was used to perform “leaf dips” and ultrathin sections. Electron microscopy of these preparations revealed membrane-bound bullet-shaped particles characteristic of the Rhabdoviridae family in mesophyll cytoplasm and vascular bundle parenchyma. The virus was experimentally transmitted to healthy 9-day-old corn plants, with Peregrinus maidis (order Hemiptera, family Delphacidae) raised under laboratory conditions using acquisition, latency, and inoculation vector periods of 7, 21, and 7 days, respectively. The field observed symptoms were replicated in the transmitted plants. Total RNA was extracted from symptomatic and asymptomatic plants with the RNeasy Plant Mini Kit (Qiagen, Germany), and one step RT-PCR (Access RTPCR Kit, Promega, Madison, WI) was performed, using two sets of degenerate primers targeting conserved regions of rhabdovirus L polymerase gene, primers PVO (1) and Rhab (2). The agarose gel bands shown only in symptomatic samples were 450 bp (1) and 1,000 bp (2), as expected. The approximately 1 kb amplicon, which includes that of 450 bp, was cloned into pGEM-T Easy Vector System (Promega). Five independent clones were sequenced in both directions with M13 F/R universal primers to generate a consensus sequence (GenBank Accession No. JQ715419), which was compared to similar plant rhabdovirus sequences available on GenBank. The partial L polymerase gene sequence of the corn rhabdovirus, Maize yellow striate virus had 73% and 71% sequence identity with the members of the Cytorhabdovirus genus Barley yellow striate mosaic virus isolate Zanjan-1 (BYSMV; GenBank Accession No. FJ665628) and Northern cereal mosaic virus (NCMV; GenBank Accession No. NC002251), respectively. A phylogenetic tree from the partial nucleotide L polymerase sequence indicates that the rhabdovirus infecting maize in Argentina is closely related to the cytorhabdovirus members and is separated from the nucleorhabdovirus group. To our knowledge, this is the first mention of a Rhabdoviridae family virus infecting maize detected in Argentina.Fil: Maurino, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Laguna, Irma Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Nome, Claudia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Giménez, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentin

    Estado actual de la sanidad del cultivo de maíz en Argentina

    No full text
    En el país, un factor relevante para la difusión de las enfermedades en el maíz lo constituye el desarrollo y adopción de nuevos escenarios tecnológicos productivos con la incorporación masiva de sistemas de labranza que conservan el suelo, el agua y reducen la erosión hídrica, como la siembra directa y la labranza mínima, presencia de rastrojo en superficie, sustrato de sobrevivencia de patógenos, uso de importantes cantidades de fertilizantes y fitosanitarios como herbicidas, insecticidas y fungicidas, con escasos análisis de fertilidad inicial o sin monitoreo adecuado, cambios en las fechas de siembra, uso de genotipos seleccionados en climas templados, los cuales son empleados en áreas tropicales y subtropicales y viceversa, modificación del distanciamiento entre surcos, ocurrencia de eventos transgénicos y presencia de maíces voluntarios o guachos que constituyen puentes verdes de patógenos, mayor presencia de malezas poáceas con tolerancia a glifosato y posibles reservorios de patógenos de cultivos extensivos. En el mundo se han reportado 32 virus afectando al cultivo de maíz, registrándose en Argentina la presencia de 8 de ellos (Laguna & Giménez Pecci, 2012; Maurino et al, 2014). Así mismo en el país están presentes en maíz enfermedades causadas por Mollicutes: Spiroplasma kunkelii y fitoplasmas (Carpane et al, 2012). Los hongos son un importante grupo de patógenos en maíz en Argentina, ellos pueden ocasionar infecciones severas en hojas, espigas, tallos y raíces, ocasionando en algunos casos la muerte total de la planta. Se propone en esta publicación presentar un panorama actualizado de las enfermedades del maíz en Argentina causadas por virus, Spiroplasma kunkelii y hongos.Instituto de Patología VegetalFil: Barontini, Javier Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Raspanti, Jorge Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Maurino, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Maurino, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrer Lanfranchi, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Laguna, Irma Graciela. nstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Laguna, Irma Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentin

    Infection with Spiroplasma kunkelii on temperate and tropical x temperate maize in Argentina and development of a tool to evaluate germplasm

    No full text
    Corn stunt disease is a disease that extends from the southern United States to Argentina and is one of the most important diseases in warm subtropical areas of the continent, periodically causing yield reductions in maize. The aims of this study were to develop a severity scale, estimate the effect of corn stunt caused by Spiroplasma kunkelii in maize hybrids, and identify attributes related to yield loss that would be useful to evaluate germplasms. Under artificial inoculation conditions with infective and non-infective insects, symptoms were grouped to develop and validate a scale and a severity index. Disease severity, moment of appearance of the characteristic symptom, pathogen concentration, and effect on yield were correlated for eight temperate and temperate x tropical commercial and precommercial hybrid maize germplasms. We developed and validated a 7-score severity scale. The characteristic symptom of S. kunkelii infection occurs earlier in the temperate germplasm than in the tropical x temperate germplasm. The performance of two of the four temperate germplasms was remarkable compared to the hybrids. Severity and pathogen concentration were correlated with each other, and both correlated with yield and with yield reduction. The moment of appearance of the characteristic disease symptom did not correlate with the other attributes. The severity index and the pathogen concentration may be useful to evaluate germplasm.Instituto de Patología VegetalFil: Barontini, Javier Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Barontini, Javier Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Peña Malavera, Andrea. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Consejo Nacional de Investigaciones Científicas y Técnicas . Estación Experimental Agroindustrial Obispo Colombres (EEAOC) ; TucumánFil: Ferrer Lanfranchi, Mariana. Innovaciones tecnológicas Agropecuarias S.A. (INTEA); ArgentinaFil: Ferrer Lanfranchi, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Torrico Ramallo, Ada Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Maurino, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Maurino, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Gimenez, Maria De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentin

    Complete genome sequence of maize yellow striate virus, a new cytorhabdovirus infecting maize and wheat crops in Argentina

    No full text
    A rhabdovirus infecting maize and wheat cropsin Argentina was molecularly characterized. Through nextgeneration sequencing (NGS) of symptomatic leaf samples,the complete genome was obtained of two isolates of maizeyellow striate virus (MYSV), a putative new rhabdovirus,differing by only 0.4% at the nucleotide level. The MYSVgenome consists of 12,654 nucleotides for maize and wheatvirus isolates, and shares 71% nucleotide sequence identitywith the complete genome of barley yellow striate mosaicvirus (BYSMV, NC028244). Ten open reading frames(ORFs) were predicted in the MYSV genome from theantigenomic strand and were compared with their BYSMVcounterparts. The highest amino acid sequence identity ofthe MYSV and BYSMV proteins was 80% between the Lproteins, and the lowest was 37% between the proteins 4.Phylogenetic analysis suggested that the MYSV isolatesare new members of the genus Cytorhabdovirus, familyRhabdoviridae. Yellow striate, affecting maize and wheatcrops in Argentina, is an emergent disease that presents apotential economic risk for these widely distributed crops.Fil: Maurino, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dumón, Analía Delina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Llauger, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Alemandri, Vanina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: de Haro, Luis Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Mattio, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: del Vas, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Laguna, Irma Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Giménez, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentin

    First report of Rice stripe necrosis virus in Argentina

    No full text
    Since 2008/2009, we have seen symptoms of viral infection in rice plants from crop regions in Argentina. The main objective of this work was to identify the causal agent of the rice plants’ crinkling. We therefore analyzed samples of symptomatic rice plants from Berón de Astrada (Corrientes province) and San Javier (Santa Fe province) by serological, electron microscopy and RT-PCR methods, and determined the presence of Rice stripe necrosis virus (RSNV). Helically constructed rod-shaped virus particles, with an axial canal with an estimated modal length of 228 nm, 370 nm and a last interval of 87–181 nm and a diameter of 20 nm, were observed in ultrathin sections and ISEM-D from rice leaf samples. Transmission of RSNV by viruliferous soil was achieved. Phylogenetic analysis, based on a partial sequence (828 nt) of the helicase gene of 10 RSNV isolates, grouped the Argentinian sequences in the same clade as the African, rather than with the sequence from Colombia. This is the first report not only of the presence of RSNV in Argentina, but of a viral infection in rice plants in the country.Fil: Maurino, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Giménez, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Kruger, Raul Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Corrientes. Estación Experimental Agropecuaria Corrientes; ArgentinaFil: Cúndom, María A.. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Gutiérrez, Susana Alejandra. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentin

    First report of rice stripe necrosis virus in Argentina

    No full text
    Since 2008/2009, we have seen symptoms of viral infection in rice plants from crop regions in Argentina. The main objective of this work was to identify the causal agent of the rice plants' crinkling. We therefore analyzed samples of symptomatic rice plants from Berón de Astrada (Corrientes province) and San Javier (Santa Fe province) by serological, electron microscopy and RT-PCR methods, and determined the presence of Rice stripe necrosis virus (RSNV). Helically constructed rod-shaped virus particles, with an axial canal with an estimated modal length of 228 nm, 370 nm and a last interval of 87–181 nm and a diameter of 20 nm, were observed in ultrathin sections and ISEM-D from rice leaf samples. Transmission of RSNV by viruliferous soil was achieved. Phylogenetic analysis, based on a partial sequence (828 nt) of the helicase gene of 10 RSNV isolates, grouped the Argentinian sequences in the same clade as the African, rather than with the sequence from Colombia. This is the first report not only of the presence of RSNV in Argentina, but of a viral infection in rice plants in the countryInstituto de Patología VegetalFil: Maurino, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gimenez, Maria de la Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Kruger, Raúl. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Corrientes; ArgentinaFil: Cúndom, María Águeda. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Gutierrez, Susana Alejandra. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

    Infection with Spiroplasma kunkelii on temperate and tropical x temperate maize in Argentina and development of a tool to evaluate germplasm

    No full text
    Corn stunt disease is a disease that extends from the southern United States to Argentina and is one of the most important diseases in warm subtropical areas of the continent, periodically causing yield reductions in maize. The aims of this study were to develop a severity scale, estimate the effect of corn stunt caused by Spiroplasma kunkelii in maize hybrids, and identify attributes related to yield loss that would be useful to evaluate germplasms. Under artificial inoculation conditions with infective and non-infective insects, symptoms were grouped to develop and validate a scale and a severity index. Disease severity, moment of appearance of the characteristic symptom, pathogen concentration, and effect on yield were correlated for eight temperate and temperate x tropical commercial and precommercial hybrid maize germplasms. We developed and validated a 7-score severity scale. The characteristic symptom of S. kunkelii infection occurs earlier in the temperate germplasm than in the tropical x temperate germplasm. The performance of two of the four temperate germplasms was remarkable compared to the hybrids. Severity and pathogen concentration were correlated with each other, and both correlated with yield and with yield reduction. The moment of appearance of the characteristic disease symptom did not correlate with the other attributes. The severity index and the pathogen concentration may be useful to evaluate germplasm.Fil: Barontini, Javier Miguel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; ArgentinaFil: Peña Malavera, Andrea Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Ferrer, Mariana. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; ArgentinaFil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; ArgentinaFil: Maurino, Maria Fernanda. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; ArgentinaFil: de la Paz Giménez Pecci, María. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; Argentin

    Fungal screening and aflatoxin production by Aspergillus section Flavi isolated from pre-harvest maize ears grown in two Argentine regions

    No full text
    Aflatoxin contamination in maize kernels grown in central Argentina has increased since 2008. Pre-harvest maize ear samples were collected during two growing seasons to determine prevalent fungal genera as well as aflatoxin production capacity by Aspergillus sect. Flavi in two maize regions. A direct planting procedure was performed for fungal screening. Aspergillus section Flavi was identified via a morphological analysis and some colonies were isolated. Strains were subjected to molecular analysis and aflatoxin production was studied. Fusarium was the fungus genus with the highest severity, followed by Penicillium and Aspergillus in both regions. Aspergillus sect. flavi were detected in 27 (73%) samples. PCR amplifications were observed in 94% of the isolates. Aspergillus sect. flavi severity varied significantly between years. A great proportion of toxigenic strains were observed in a native Aspergillus sect. flavi population (95%) producing B- and G-type aflatoxins. Maize ears grown in regions I and IV in Argentina are prone to degradation by fungi. Toxigenic strains are predominant in native Aspergillus sect. Flavi populations. Aflatoxin contamination of maize kernels is not restricted to post-harvest; rather, it is a serious issue that begins with colonization by Aspergillus sect. Flavi in ears.Fil: Camiletti, Boris Xavier. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maurino, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cristos, Diego Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria; ArgentinaFil: Magnoli, Carina Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lucini, Enrique Iván. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Giménez Pecci, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentin

    Corn stunt spiroplasma en la Región Subtropical

    No full text
    Dada la importancia del Corn stunt spiroplasma para la producción de maíz en la región subtropical, la siguiente información tiene el objetivo de presentar los valores de incidencia y prevalencia de este patógeno en la campaña 2014/2015 y analizar su comportamiento en los últimos 5 años.Fil: Druetta, Marcelo Alberto. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Raspanti, Jorge Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Luna Perez, Ignacio Martín. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Barontini, Javier Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Maurino, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Ferrer, M.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Laguna, Irma Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Giménez, María de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentin
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