The duration of intra- and inter-specific copulations between Tetranychus urticae and T. evansi

Data sheet used in the analyses of the copulation duration in intra- and inter-specific crosses. "Name" shows the name of the pair. "Experiment" shows the pair of crossing experiment: EE is T. evansi (♀) × T. evansi (♂), EU is T. evansi (♀) × T. urticae (♂), UU is T. urticae (♀) × T. urticae (♂), and UE is T. urticae (♀) × T. evansi (♂). "Female" and "Male" show female species and male species of the pair: E is T. evansi and U is T. urticae. "1stCop" shows the duration of copulations. "Comb" shows the crossing type: "Intra" is intraspecific crossing and "Inter" is interspecific crossing

R script in the analysis of male choice test

R script in the analysis of male choice tes

The numbers of eggs oviposited by mothers and the numbers of daughters and sons brought up from the eggs in the intra- and inter-specific crossing between Tetranychus urticae and T. evansi.

Data sheet used in the analyses of the number of eggs, the juvenile survival rate of offspring and the female sex ratio among offsprings produced by females mated with con- or hetero-specific males or virgin females. "Name" shows the name of the pair. "Experiment" shows the pair of crossing experiment: EE is T. evansi (♀) × T. evansi (♂), EU is T. evansi (♀) × T. urticae (♂), UU is T. urticae (♀) × T. urticae (♂), UE is T. urticae (♀) × T. evansi (♂), U is virgin oviposition by T. urticae (♀) and E is virgin oviposition by T. evansi (♀). "Pfemale" shows the species of mother: E is T. evansi and U is T. urticae. "Comb" shows the crossing type: "Intra" is intraspecific crossing, "Inter" is interspecific crossing and "Unmated" is virgin oviposition. "Male" shows the number of sons. "Female" shows the number of daughters. "Deadlarva" is the number of offspring that died before developing to adult stage. "TotalEgg" shows the number of eggs oviposited by the mother

The average (+ s.e.m.) numbers of predatory mites (<i>A. swirskii</i>) on plants 28 days after they were released.

<p>(a) Distance between neighbouring plants 0.6 m. Predators were released on the plant at 0 m, on which whiteflies were also released. The average number of predators differed among plants (GLM, F_4,10 = 14.7, P<0.001). (b) Distance between neighbouring plants 2 m. Predators were either released on the plant on which whiteflies were released at 0 m (Predators at 0 m), on the plant at 2 m (Predators at 2 m) or both on the plant at 0 and 2 m (Predators at 0 and 2 m). The average number of predators differed among plants in all three treatments (Predators at 0 m: F_4,15 = 44.0, P<0.001; Predators at 2 m: F_4,15 = 17.3, P<0.001; Predators at 0 and 2 m: F_4,15 = 12.4, P<0.001). Within each treatment, bars with different letters differ significantly (contrasts after model simplification). See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045487#pone-0045487-t001" target="_blank">Table 1</a> for a summary of the release schedule.</p

Population dynamics of Tetranychus urticae and T. evansi on tomato plants (def-1) over a period of 4 weeks after mite introduction

Data sheet used in the comparisons of the number of female mites on tomato plants over week among treatments. "Exp" shows the treatment: A is Strong RI, B is Control 1, C is Control 2, D is Mild RI, E is Control 3 and F is Control 4 in Table 1 in the paper. "PotNo" and "PotNo2" show the name or the number of the trials. "DifSp" shows species of the founder females: E is T. evansi and U is T. urticae. "Female" shows the reproductive status of the founder females, unmated or mated. "Week" shows the time when the number of mites was counted. "NO" shows the number of mites. "NO2" shows the number of mites per the number of founder females

The number of pairs where intra- and inter-specific copulations occurred between Tetranychus urticae and T. evansi

Data sheet used in the statistic analyses of the number of pairs where intra- and inter-specific copulations occurred within 0.5 h after introduction of a virgin female and three virgin males onto a tomato leaf arena. "Experiment" shows the pair of crossing experiment: EE is T. evansi (♀) × T. evansi (♂), EU is T. evansi (♀) × T. urticae (♂), UU is T. urticae (♀) × T. urticae (♂), and UE is T. urticae (♀) × T. evansi (♂). "Female" and "Male" show female species and male species of the pair: E is T. evansi and U is T. urticae. "Yes" shows the number of pair where copulations occurred. "No" shows the number of pair where copulations did not occure. "Comb" shows the crossing type: "Intra" is intraspecific crossing and "Inter" is interspecific crossing

Summary of the release schedule of the experiments.

1<p>Distance between neighbouring plants in m;</p>2<p>Number of replicates;</p>3<p>The first plant of the row of five;</p>4<p>The second plant;</p>5<p>Number of adult whiteflies released;</p>6<p>Number of adult female predatory mites released.</p><p>Whiteflies were always released on the first plant (at 0 m) of a row of 5 plants. Female predatory mites were released on the first, second, or both plants. No whiteflies or predators were released on the other 3 plants.</p

The average (± s.e.m.) numbers of adult whiteflies that dispersed from a release plant to 4 neighbouring plants through time.

<p>(a) Distance between neighbouring plants 0.6 m. Predators were either released on the same plant as the whiteflies (at 0 m) or no predators were released (none). (b) Distance between neighbouring plants 2 m. Predators were either released on the same plant as the whiteflies (at 0 m), on the plant closest to the release plant (hence, at 2 m), on the same plant as the whiteflies and on the neighbouring plant (at 0 and 2 m), or no predators were released (none). See legend to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045487#pone-0045487-g001" target="_blank">Figure 1</a> for further explanation.</p

The average (± s.e.m.) numbers of adult whiteflies that dispersed to plants at various distances from the plant on which they were released as a function of time since release.

<p>(a) Distance between neighbouring plants 0.6 m, treatment with predators on the release plant. (b) Distance 0.6 m, treatment without predators. Significantly more prey were recaptured through time on the plant at 0.6 m. The two treatments are shown separately to demonstrate the absence of an interaction of treatment with the distance dispersed. (c) Distance between neighbouring plants 2 m. The numbers of prey recaptured did not differ significantly among plants.</p

The average number of adult whiteflies (+ s.e.m.) on all five plants at the end of the experiment (after 28 days).

<p>Symbols above the bars indicate significant differences. Whitefly numbers were significantly higher in treatments in which predators were not released on the same plant as the whiteflies (left-hand two bars) than in treatments where predators and whiteflies were released on the same plant.</p