SMSLib - biblioteca C++ do Sting Millennium Suite.

Organização lógica do SMS. Descrição da SMSLib. Leitura de arquivos PDB. Leitura de arquivos HSSP. Leitura de arquivos com parâmetros simples. Cálculo e leitura de contatos. Cálculo e leitura de Dihedral Angels.bitstream/CNPTIA/9898/1/comuntec39.pdfAcesso em: 30 maio 2008

Vaginal lactobacilli and vaginal dysbiosis-associated bacteria differently affect cervical epithelial and immune homeostasis and anti-viral defenses

Persistent infection with High Risk-Human Papilloma Viruses (HR-HPVs) is a primary cause of cervical cancer worldwide. Vaginal-dysbiosis-associated bacteria were correlated with the persistence of HR-HPVs infection and with increased cancer risk. We obtained strains of the most represented bacterial species in vaginal microbiota and evaluated their effects on the survival of cervical epithelial cells and immune homeostasis. The contribution of each species to supporting the antiviral response was also studied. Epithelial cell viability was affected by culture supernatants of most vaginal-dysbiosis bacteria, whereas Lactobacillus gasseri or Lactobacillus jensenii resulted in the best stimulus to induce interferon-γ (IFN-γ) production by human mononuclear cells from peripheral blood (PBMCs). Although vaginal-dysbiosis-associated bacteria induced the IFN-γ production, they were also optimal stimuli to interleukin-17 (IL-17) production. A positive correlation between IL-17 and IFN-γ secretion was observed in cultures of PBMCs with all vaginal-dysbiosis-associated bacteria suggesting that the adaptive immune response induced by these strains is not dominated by T(H)1 differentiation with reduced availability of IFN-γ, cytokine most effective in supporting virus clearance. Based on these results, we suggest that a vaginal microbiota dominated by lactobacilli, especially by L. gasseri or L. jensenii, may be able to assist immune cells with clearing HPV infection, bypasses the viral escape and restores immune homeostasis

Abdominal CT: a radiologist-driven adjustment of the dose of iodinated contrast agent approaches a calculation per lean body weight

BACKGROUND: The contrast agent (CA) dose for abdominal computed tomography (CT) is typically based on patient total body weight (TBW), ignoring adipose tissue distribution. We report on our experience of dosing according to the lean body weight (LBW). METHODS: After Ethics Committee approval, we retrospectively screened 219 consecutive patients, 18 being excluded for not matching the inclusion criteria. Thus, 201 were analysed (106 males), all undergoing a contrast-enhanced abdominal CT with iopamidol (370 mgI/mL) or iomeprol (400 mgI/mL). LBW was estimated using validated formulas. Liver contrast-enhancement (CEL) was measured. Data were reported as mean\u2009\ub1\u2009standard deviation. Pearson correlation coefficient, ANOVA, and the Levene test were used. RESULTS: Mean age was 66\u2009\ub1\u200913 years, TBW 72\u2009\ub1\u200915 kg, LBW 53\u2009\ub1\u200911 kg, and LBW/TBW ratio 74\u2009\ub1\u20098%; body mass index was 26\u2009\ub1\u20095 kg/m2, with 9 underweight patients (4%), 82 normal weight (41%), 76 overweight (38%), and 34 obese (17%). The administered CA dose was 0.46\u2009\ub1\u20090.06 gI/kg of TBW, corresponding to 0.63\u2009\ub1\u20090.09 gI/kg of LBW. A negative correlation was found between TBW and CA dose (r\u2009=\u2009-0.683, p\u2009<\u20090.001). CEL (Hounsfield units) was 51\u2009\ub1\u200918 in underweight patients, 44\u2009\ub1\u20098 in normal weight, 42\u2009\ub1\u20099 in overweight, and 40\u2009\ub1\u20096 in obese, with a significant difference for both mean (p\u2009=\u20090.004) and variance (p\u2009<\u20090.001). A low but significant positive correlation was found between CEL and CA dose in gI per TBW (r\u2009=\u20090.371, p\u2009<\u20090.001) or per LBW (r\u2009=\u20090.333, p\u2009<\u20090.001). CONCLUSIONS: The injected CA dose was highly variable, with obese patients receiving a lower dose than underweight patients, as a radiologist-driven 'compensation effect'. Diagnostic abdomen CT examinations may be obtained using 0.63 gI/kg of LBW

Role of interventional radiology in the management of complications after pancreatic surgery: a pictorial review

Pancreatic resections are surgical procedures associated with high incidence of complications, with relevant morbidity and mortality even at high volume centres. A multidisciplinary approach is essential in the management of these events and interventional radiology plays a crucial role in the treatment of patients developing post-surgical complications. This paper offers an overview on the interventional radiological procedures that can be performed to treat different type of complications after pancreatic resection. Procedures such as percutaneous drainage of fluid collections, percutaneous transhepatic biliary procedures, arterial embolisation, venous interventions and fistula embolisation are viable treatment options, with fewer complications compared with re-look surgery, shorter hospital stay and faster recovery. A selection of cases of complications following pancreatic surgery managed with interventional radiological procedure are presented and discussed. Teaching Points \u2022 Interventional radiology is crucial to treat complications after pancreatic surgery \u2022 Percutaneous drainage of collections can be performed under ultrasound or computed tomography guidance \u2022 Percutaneous biliary procedures can be used to treat biliary complications \u2022 Venous procedures can be performed effectively through transhepatic or transjugular access \u2022 Fistulas can be treated effectively by percutaneous embolisation

STING Millennium: a web-based suite of programs for comprehensive and simultaneous analysis of protein structure and sequence.

Sting Millennium suite intrinsics. SMS organization. Sting Millennium Modes. Sting Millennium Modules. Millennium Features. Example of Sting Millennium Application.Na publicação: Paula R. Kuser