Prospective observational study of point-of-care creatinine in trauma.

Background:Patients with trauma are at risk for renal dysfunction from hypovolemia or urological injury. In austere environments, creatinine values are not available to guide resuscitation. A new portable device, the Stat Sensor Point-of-care (POC) Whole Blood Creatinine Analyzer, provides accurate results in <30 s and requires minimal training. This device has not been evaluated in trauma despite the theoretical benefit it provides. The purpose of this study is to determine the clinical impact of the POC device in trauma. Methods:40 patients with trauma were enrolled in a prospective observational study. One drop of blood was used for creatinine determination on the Statsensor POC device. POC creatinine results were compared to the laboratory. Turnaround time (TAT) for POC and laboratory methods was calculated as well as time elapsed to CT scan if applicable. Results:Patients (n=40) were enrolled between December 2014 and March 2015. POC creatinine values were similar to laboratory methods with a mean bias of 0.075±0.27 (p=0.08). Mean analytical TATs for the POC measurements were significantly faster than the laboratory method (11.6±10.0 min vs 78.1±27.9 min, n=40, p<0.0001). Mean elapsed time before arrival at the CT scanner was 52.9±34.2 min. Conclusions:The POC device reported similar creatinine values to the laboratory and provided significantly faster results. POC creatinine testing is a promising development for trauma practice in austere environments and workup of a subset of stable patients with trauma. Further study is warranted to determine clinical impact, both in hospital-based trauma and austere environments

Hubble Space Telescope Systems Engineering Case Study

The Hubble Space Telescope (HST) is an orbiting astronomical observatory operating in the spectrum from the near-infrared into the ultraviolet. Launched in 1990 and scheduled to operate through 2010, HST carries and has carried a wide variety of instruments producing imaging, spectrographic, astrometric, and photometric data through both pointed and parallel observing programs. Over 100,000 observations of more than 20,000 targets have been produced for retrieval. A macroscopic, cumulative representation of these observations is shown in the figure below to provide a sense of the enormous volume of astronomical data collected by the HST about our universe, our beginnings, and, consequently, about our future. The telescope is already well known as a marvel of science. This case study hopes to represent the facet of the HST that is a marvel of systems engineering, which, in fact, generated the scientific research and observation capabilities now appreciated worldwide

Analyses of Groundwater for Trace Levels of Pesticides

Agricultural production is a major source of revenue in Arkansas. In order to increase productivity, it has been necessary to rely increasingly on the use of pesticides and irrigation water. In the last 15 years several states have reported finding pesticides in groundwater as a result of normal agricultural practices. Since almost half of the population also relies on groundwater as their source of drinking water, it is necessary to conduct research as to ascertain the presence or absence of commonly used pesticides in groundwater. Multi residue analytical techniques were developed for the analysis of acifluorfen, alachlor, atrazine, cyanazine, diuron, fluometuron, linuron, metolachlor and propanil from groundwater, by either GLC or HPLC. Analytical sensitivities ranged from 1 to 5 ppb. Groundwater samples were collected from three areas of southeastern Arkansas that are under heavy agricultural production. Samples were collected directly from irrigation wells just prior to and during the peak of the irrigation season and will be compared to determine whether any temporal differences exist. To-date, over 500 samples have been analyzed. No positive finding for any pesticide has been shown

Inclusion Complexes of Chain Molecules with Cycloamyloses III. Molecular Dynamics Simulations of Polyrotaxanes Formed by Poly(propylene glycol) and beta-Cyclodextrins

Molecular dynamics simulations were performed in vacuo on “channel type” polyrotaxanes composed of β-cyclodextrins (βCDs) threaded onto isotactic and syndiotactic poly(propylene glycol) (PPG). In the most stable complex, the βCDs form a close-packed structure from one end of the PPG chain to the other. Non-bonded van der Waals interactions between βCD and PPG are the main source of stabilization of the complex. Head-to-head and tail-to-tail orientation of successive βCDs in the complex is more favorable than a head-to-tail orientation, due to intermolecular hydrogen bonding between head-to-head βCD units. βCDs in polyrotaxanes adopt a more rigid and symmetrical macroring conformation than does an isolated βCD. Formation of the polyrotaxane is accompanied by an increase in the number of trans states at the bonds in the backbone of PPG. For this reason, the PPG chain in the polyrotaxane is much more extended than the unperturbed chain.This work was supported by DGICYT PG94-0364 and by National Science Foundation grant DMR 952327

Sediment Loading and Water Quality of Field Run-off Water

Intensive tillage is commonly employed in many agronomic production systems in the United States. Tillage operations may include disking the field, re-smoothing the soil, seedbed formation, reducing the seedbeds, and shallow cultivation for weed control. Tillage practices in conjunction with rainfall have been linked to soil erosion, which may adversely affect the environment. The soil erosion dynamics of two large-scale production cotton fields that utilized both modern-conventional and conservation-tillage technology were examined. Studies were conducted in the cotton-producing region of southeast Arkansas in the Bayou Bartholomew watershed. Bayou Bartholomew is currently listed by the United States Environmental Protection Agency as an impacted stream. The soils at these sites were related, coarse-textured alfisols. One field was cropped to conventionally tilled cotton and intensively tilled. The second field was cropped to cotton using modern conservation tillage technology. Both fields were furrow-flow irrigated using piped water. Intense rainfall usually occurs in the Mississippi River Delta Region, particularly in the winter and spring months. Conservation tillage proved to be immediately beneficial in controlling soil erosion and sediment loss due to field run-off water from rainfall. Sediment content of run-off water induced by rainfall from the conventionally tilled cotton field was significantly greater than the sediments found in run-off water from the conservation tilled cotton field. The amount of sediment found in rainfall run-off water decreased more rapidly with time under conservation tillage than under conventional tillage. The tillage system made little difference in sediment content of run-off water from irrigation. The water flow from furrow irrigation is typically slow and steady. There is no droplet impact on the ground from furrow-flow irrigation as there would be from rainfall. Apparently, the gentle flow of the water down the furrows was insufficient to dislodge large numbers of soil particles

Water Quality as Affected by Pesticides in Rice Production

Studies were conducted to determine the environmental persistence of the rice pesticides triclopyr, 2,4-D, benomyl and quinclorac. Triclopyr half-lives ranged from \u3c7 d to \u3e100 d depending on depth within the soil profile and clay content. Triclopyr persistence increased as depth within the profile increased and clay content increased. The benomyl metabolite MBC was present at greater than 50% of the initial amount after 9 months in the field. In simulated carryover field studies quinclorac exhibited the greatest potential for injury to subsequent rotational crops. Cotton and soybean growth was reduced when planted at four weeks after quinclorac application. The adsorption of triclopyr to three soils was measured by the batch equilibrium technique. Freundlich isotherms were linear and resulted in Kf values of 1.60, 1.41, and 2.75 for Crowley silt loam soil from depths of 0, 0.2, and 0.6 m, respectively, within soil profile. Soil thin-layer chromatography of triclopyr resulted in Rf values of 0.42, 0.69, and 0.40 for the Crowley silt loam soil from 0, 0.2, and 0.6 m depths. In controlled temperature and water potential degradation studies, triclopyr and 2,4-D degraded more rapidly at 3 0 C than at 15 C. The degradation rates of the two herbicides responded oppositely to water potential. 2,4-D degraded more rapidly under anaerobic conditions, whereas triclopyr degraded more rapidly under aerobic conditions

Calculation of the Phase Behavior of Lipids

The self-assembly of monoacyl lipids in solution is studied employing a model in which the lipid's hydrocarbon tail is described within the Rotational Isomeric State framework and is attached to a simple hydrophilic head. Mean-field theory is employed, and the necessary partition function of a single lipid is obtained via a partial enumeration over a large sample of molecular conformations. The influence of the lipid architecture on the transition between the lamellar and inverted-hexagonal phases is calculated, and qualitative agreement with experiment is found.Comment: to appear in Phys.Rev.

Regulation of Sodium Channel Function by Bilayer Elasticity: The Importance of Hydrophobic Coupling. Effects of Micelle-forming Amphiphiles and Cholesterol

Membrane proteins are regulated by the lipid bilayer composition. Specific lipid–protein interactions rarely are involved, which suggests that the regulation is due to changes in some general bilayer property (or properties). The hydrophobic coupling between a membrane-spanning protein and the surrounding bilayer means that protein conformational changes may be associated with a reversible, local bilayer deformation. Lipid bilayers are elastic bodies, and the energetic cost of the bilayer deformation contributes to the total energetic cost of the protein conformational change. The energetics and kinetics of the protein conformational changes therefore will be regulated by the bilayer elasticity, which is determined by the lipid composition. This hydrophobic coupling mechanism has been studied extensively in gramicidin channels, where the channel–bilayer hydrophobic interactions link a “conformational” change (the monomer↔dimer transition) to an elastic bilayer deformation. Gramicidin channels thus are regulated by the lipid bilayer elastic properties (thickness, monolayer equilibrium curvature, and compression and bending moduli). To investigate whether this hydrophobic coupling mechanism could be a general mechanism regulating membrane protein function, we examined whether voltage-dependent skeletal-muscle sodium channels, expressed in HEK293 cells, are regulated by bilayer elasticity, as monitored using gramicidin A (gA) channels. Nonphysiological amphiphiles (β-octyl-glucoside, Genapol X-100, Triton X-100, and reduced Triton X-100) that make lipid bilayers less “stiff”, as measured using gA channels, shift the voltage dependence of sodium channel inactivation toward more hyperpolarized potentials. At low amphiphile concentration, the magnitude of the shift is linearly correlated to the change in gA channel lifetime. Cholesterol-depletion, which also reduces bilayer stiffness, causes a similar shift in sodium channel inactivation. These results provide strong support for the notion that bilayer–protein hydrophobic coupling allows the bilayer elastic properties to regulate membrane protein function