La Rete Integrata Nazionale GPS (RING) dell' INGV: una infrastruttura aperta per la ricerca scientifica

Since 2004, the Istituto Nazionale di Geofisica e Vulcanologia (INGV) is investing important energies for the creation of a continuous GPS network dislocated all over the Italian territory. Data transmission will occur in real time, integrating the experiences already existing in the different INGV institutes and developing a 3-yrs strategy for the new installations. The main targets of the network are represented by active tectonics studies, including also the seismological part as strain accumulation on faults. Within a 3-yrs funding project, it is expected, to realize for the scientific community an infrastructure which is comparable to those existing in countries where advanced crustal deformation studies are carried out. Thus, INGV have co-located the classical seismological instrumentation (broad band seismometers and accelerometers) with GPS receivers to observe and quantify the whole seismic cycle. In this short paper, we describe the CGPS network, the technological choices for the monumentation and the data transmission, the data and metadata management and, finally, the data policy and the deliverables.INGVUnpublishedreserve

The RING GPS network: a research geodetic infrastructure to study plate boundary deformation in the Central Mediterranean

We present the INGV (Italian National Institute of Geophysics and Volcanology) geodetic research infrastructure and related facilities, dedicated to the observation and monitoring of current deformation of the plate boundary between Africa and Eurasia. The recent increase of continuous GPS (CGPS) stations in the Central Mediterranean plate boundary zone offers the opportunity to study in detail the present-day kinematics of this actively deforming region. For answering all the open questions related to this complex area, INGV deployed a permanent, integrated and real-time monitoring CGPS network (RING) all over Italy. The RING network (http:/ring.gm.ingv.it) is now constituted by more than 150 stations. All stations have high quality GPS monuments and most of them are co-located with broadband or very broadband seismometers and strong motion sensors. The RING CGPS sites acquire at 1Hz and 30s sampling rates (some of them acquire at 10 Hz) and are connected in real-time to the INGV acquisition centers located in Roma and Grottaminarda. Real-time GPS data are transmitted using different systems, such as satellite systems, Internet, GPRS/UMTS and wireless networks. The differentiation of data transmission type and the integration with seismic instruments makes this network one of the most innovative CGPS networks in Europe. Furthermore, the INGV data acquisition centers acquire, archive and analyze most of the Italian CGPS stations managed by regional or national data providers (such as local Authorities and nation-wide industries), integrating more than 350 stations of the CGPS scientific and commercial networks existing in the Italian region. To manage data acquisition, storage, distribution and access we developed dedicated facilities including new softwares for data acquisition and a web-based collaborative environment for management of data and metadata. The GPS analysis is carried out with the three main geodetic-quality softwares used in the GPS scientific community: Bernese GAMIT an GIPSY-OASIS. The resulting daily solutions are aligned to the ITRF2005 reference frame. Stable plate reference frames are realized by minimizing the horizontal velocities at sites on the Eurasia and Nubia plates, respectively. The different software-related solutions consistency RMS is within 0.3 mm/yr (Avallone et al., 2010). The solutions are then evaluated with regard to the numerous scientific motivations behind this presentation, ranging from the definition of strain distribution and microplate kinematics within the plate boundary, to the evaluation of tectonic strain accumulation on active faults. The RING network is strongly contributing to the definition of GPS velocity field in the Italian region, and now is able to furnish a newly and up to date view of this actively deforming part of the Nubia-Eurasia plate boundary. INGV is now aiming to make the RING (and integrated CGPS networks) data and related products publicly available for the scientific community. We believe that our network represents an important reality in the framework of the EPOS infrastructure and we strongly support the idea of an European research approach to data sharing among the scientific community. We will present (a) the current CGPS site distribution, (b) the technological description of the data acquisition, storage and distribution at INGV centers, (c) the results of CGPS data analysis, and (d) the planned data access for the scientific community.PublishedVienna, Geophysical Research Abstracts Vol. 13, EGU2011-8626, 20111.9. Rete GPS nazionale3.2. Tettonica attivaope

The RING GPS network: a research geodetic infrastructure to study plate boundary deformation in the Central Mediterranean

We present the INGV (Italian National Institute of Geophysics and Volcanology) geodetic research infrastructure and related facilities, dedicated to the observation and monitoring of current deformation of the plate boundary between Africa and Eurasia. The recent increase of continuous GPS (CGPS) stations in the Central Mediterranean plate boundary zone offers the opportunity to study in detail the present-day kinematics of this actively deforming region. For answering all the open questions related to this complex area, INGV deployed a permanent, integrated and real-time monitoring CGPS network (RING) all over Italy. The RING network (http:/ring.gm.ingv.it) is now constituted by more than 150 stations. All stations have high quality GPS monuments and most of them are co-located with broadband or very broadband seismometers and strong motion sensors. The RING CGPS sites acquire at 1Hz and 30s sampling rates (some of them acquire at 10 Hz) and are connected in real-time to the INGV acquisition centers located in Roma and Grottaminarda. Real-time GPS data are transmitted using different systems, such as satellite systems, Internet, GPRS/UMTS and wireless networks. The differentiation of data transmission type and the integration with seismic instruments makes this network one of the most innovative CGPS networks in Europe. Furthermore, the INGV data acquisition centers acquire, archive and analyze most of the Italian CGPS stations managed by regional or national data providers (such as local Authorities and nation-wide industries), integrating more than 350 stations of the CGPS scientific and commercial networks existing in the Italian region. To manage data acquisition, storage, distribution and access we developed dedicated facilities including new softwares for data acquisition and a web-based collaborative environment for management of data and metadata. The GPS analysis is carried out with the three main geodetic-quality softwares used in the GPS scientific community: Bernese GAMIT an GIPSY-OASIS. The resulting daily solutions are aligned to the ITRF2005 reference frame. Stable plate reference frames are realized by minimizing the horizontal velocities at sites on the Eurasia and Nubia plates, respectively. The different software-related solutions consistency RMS is within 0.3 mm/yr (Avallone et al., 2010). The solutions are then evaluated with regard to the numerous scientific motivations behind this presentation, ranging from the definition of strain distribution and microplate kinematics within the plate boundary, to the evaluation of tectonic strain accumulation on active faults. The RING network is strongly contributing to the definition of GPS velocity field in the Italian region, and now is able to furnish a newly and up to date view of this actively deforming part of the Nubia-Eurasia plate boundary. INGV is now aiming to make the RING (and integrated CGPS networks) data and related products publicly available for the scientific community. We believe that our network represents an important reality in the framework of the EPOS infrastructure and we strongly support the idea of an European research approach to data sharing among the scientific community. We will present (a) the current CGPS site distribution, (b) the technological description of the data acquisition, storage and distribution at INGV centers, (c) the results of CGPS data analysis, and (d) the planned data access for the scientific community

Interplay between Hypoxia and Extracellular Vesicles in Cancer and Inflammation

Hypoxia is a severe stress condition often observed in cancer and chronically inflamed cells and tissues. Extracellular vesicles play pivotal roles in these pathological processes and carry biomolecules that can be detected in many biofluids and may be exploited for diagnostic purposes. Several studies report the effects of hypoxia on extracellular vesicles’ release, molecular content, and biological functions in disease. This review summarizes the most recent findings in this field, highlighting the areas that warrant further investigation

Inhibition of smoothened in breast cancer cells reduces CAXII expression and cell migration

Breast cancer (BC) relapse and metastasis are the leading cause of death and, together with drug resistance, keep mortality still high. The Hedgehog (Hh) pathway is expressed during embryogenesis, organogenesis and in adult tissue homeostasis and its aberrant activation is often associated with cancer. Carbonic anhydrase (CA) enzymes are important during development; they play a key role in controlling several cellular mechanisms, such as pH regulation, survival, and migration, and they are aberrantly expressed in cancer. The goal of this study was to investigate the interplay between the Hh pathway and CAXII in terms of BC cell migration. We here demonstrated that smoothened (SMO) silencing resulted in a reduction of CAXII expression at mRNA and protein level. This led to a decrease in cell migration, which was restored when cells were treated with an SMO agonist, Sag dihydrochloride (SAG), but not when cells were cotreated with SAG and the CAs inhibitor Acetazolamide. This suggested that the ability of SAG to promote cell migration was impaired when CAXII was inhibited. The reduction was also confirmed within hypoxic and inflammatory microenvironment, typical of BC, indicating a key role of the Hh pathway in controlling CAXII expression. Our results may contribute to further understand the physiology of BC cells and indicate that the Hh pathway controls BC cell migration and cell invasion also through CAXII, with important implications in identifying novel therapeutic targets

The Shc protein RAI promotes an adaptive cell survival program in hypoxic neuroblastoma cells

Neuroblastoma (NB) is a highly malignant pediatric solid tumor where a hypoxic signature correlates with unfavorable patient outcome. The hypoxia-inducible factor (HIF)-1\u3b1 plays an important role in NB progression, contributing to cell proliferation and invasiveness. RAI belongs to the Shc family proteins, it is mainly neuron specific and protects against cerebral ischemia. RAI is also expressed in several NB cell lines, where it promotes cell survival. In this work, hypoxia differently affected cell survival and pro-apoptotic program in two NB cell lines, either expressing RAI (SKNBE) or not (SKNMC). RAI expression appeared to promote NB cell survival and to reduce some pro-apoptotic markers under hypoxia. Accordingly, the RAI silencing in SKNBE cells resulted in a reduction of cell survival and HIF-1\u3b1 expression. Furthermore, using SKNMC cells stably expressing RAI, we defined a role of RAI in NB cell responses to hypoxia. Of interest, in hypoxic SKNMC cells expressing RAI HIF-1\u3b1 protein levels were higher than in control cells. This was associated with a) an increased cell survival; b) an increased expression of anti-apoptotic markers; c) a pro-autophagic and not pro-apoptotic phenotype; and d) an increased metabolic activity. We may conclude that RAI plays an important role in hypoxic signaling in NB cells and the interplay between RAI and HIF-1\u3b1 may be relevant in the protection of NB cells against hypoxia. Our results may contribute to a further understanding the physiology of NB cells and the molecular mechanisms involved in their survival, with important implications in NB progression

Separation of Extracellular Vesicles by DNA-Directed Immunocapturing Followed by Enzymatic Release

Extracellular vesicles (EVs) have attracted great interest among researchers due to their role in cell-cell communication, disease diagnosis, and drug delivery. In spite of their potential in the medical field, there is no consensus on the best method for separating microvesicles from cell culture supernatant and complex biological fluids. Obtaining a good recovery yield and preserving physical characteristics is critical for the diagnostic and therapeutic use of EVs. The separation is made complex by the fact that blood and cell culture media, contain a large number of nanoparticles in the same size range. Methods that exploit immunoaffinity capture provide high purity samples and overcome the issues of currently used separation methods. However, the release of captured nanovesicles requires harsh conditions that hinder their use in certain types of downstream analysis. Herein, a novel capture and release approach for small extracellular vesicles (sEVs), based on DNAdirected immobilization of antiCD63 antibody is presented. The flexible DNAlinker increases the capture efficiency and allows releasing of EVs by exploiting the endonucleasic activity of DNAse I. This separation protocol works under mild conditions, enabling the release of intact vesicles that can be successfully analyzed by imaging techniques. In this article sEVs recovered from plasma were characterized by established techniques for EVs analysis including nanoparticle tracking and transmission electron microscopy.<br /

Evaluation of microbial consortia and chemical changes in spontaneous maize bran fermentation

Abstract Sustainable exploitation of agro-industrial by-products has attracted great interest in cereal bran valorization. In this research, a polyphasic approach has been carried out to characterize maize bran at microbiological and chemical level during a sourdough like fermentation process, in order to enhance its technological and nutritional properties. Autochthonous microbiota was isolated at different refreshment steps and subjected to identification and molecular characterization. Fermentation was characterized by a rapid increase in lactic acid bacteria and yeasts, with a co-dominance, at the initial stage, of Weissella spp., Pediococcus spp. and Wickerhamomyces anomalus. At the end of the fermentation, a natural selection was produced, with the prevalence of Lactobacillus plantarum, Lactobacillus brevis and Kazachstania unispora. This is the first time that a specific association between LAB and yeasts is reported, during the maize bran fermentation process. Enzymatic activities related to this microbial consortium promoted a “destructuration” of the fiber fraction, an increase in soluble dietary fiber and a reduction of phytic acid content. Our data also evidenced a noticeable increment in ferulic acid. The results obtained indicate that fermentation processes represent an efficient biotechnological approach to increase nutritional and functional potential of maize bran. Moreover, the characterization of microbiota involved in natural fermentation process will allow the selection of specific biotypes, with appropriate metabolic and enzymatic activities, to conduct “tailored” fermentation processes and improve brans or whole-meal flours from both nutritional and technological points of view

La Rete Integrata Nazionale GPS (RING) dell' INGV: una infrastruttura aperta per la ricerca scientifica

Since 2004, the Istituto Nazionale di Geofisica e Vulcanologia (INGV) is investing important energies for the creation of a continuous GPS network dislocated all over the Italian territory. Data transmission will occur in real time, integrating the experiences already existing in the different INGV institutes and developing a 3-yrs strategy for the new installations. The main targets of the network are represented by active tectonics studies, including also the seismological part as strain accumulation on faults. Within a 3-yrs funding project, it is expected, to realize for the scientific community an infrastructure which is comparable to those existing in countries where advanced crustal deformation studies are carried out. Thus, INGV have co-located the classical seismological instrumentation (broad band seismometers and accelerometers) with GPS receivers to observe and quantify the whole seismic cycle. In this short paper, we describe the CGPS network, the technological choices for the monumentation and the data transmission, the data and metadata management and, finally, the data policy and the deliverables