18 research outputs found
Tu2028 The Effect of Viral, Bacterial and Parasitic Infections on the Intestinal Microbiota
Su1193 Rectal Swabs As Feasible and Reproducible Sampling Method of the Intestinal Microbiota in a Clinical Setting
667 Temporal Stability of Faecal Microbiota By Inter Spacer rDNA Bacterial Profiling (IS-pro)
M1107 Regional Stability of Intestinal Microbiota with Inter Spacer Bacterial Profiling (IS-pro)
Rectal swabs for analysis of the intestinal microbiota.
The composition of the gut microbiota is associated with various disease states, most notably inflammatory bowel disease, obesity and malnutrition. This underlines that analysis of intestinal microbiota is potentially an interesting target for clinical diagnostics. Currently, the most commonly used sample types are feces and mucosal biopsy specimens. Because sampling method, storage and processing of samples impact microbiota analysis, each sample type has its own limitations. An ideal sample type for use in routine diagnostics should be easy to obtain in a standardized fashion without perturbation of the microbiota. Rectal swabs may satisfy these criteria, but little is known about microbiota analysis on these sample types. In this study we investigated the characteristics and applicability of rectal swabs for gut microbiota profiling in a clinical routine setting in patients presenting with various gastro-intestinal disorders. We found that rectal swabs appeared to be a convenient means of sampling the human gut microbiota. Swabs can be performed on demand, whenever a patient presents; swab-derived microbiota profiles are reproducible, whether they are gathered at home by patients or by medical professionals in an outpatient setting and may be ideally suited for clinical diagnostics and large-scale studies
W1640 Detection of Tropheryma Whipplei DNA in Intestinal Biopsy Specimens with a Novel Real-Time PCR
Clinical nutrition in the hepatogastroenterology curriculum
Contains fulltext :
172135.pdf (publisher's version ) (Open Access)Gastroenterology (GE) used to be considered a subspecialty of internal medicine. Today, GE is generally recognized as a wide-ranging specialty incorporating capacities, such as hepatology, oncology and interventional endoscopy, necessitating GE-expert differentiation. Although the European Board of Gastroenterology and Hepatology has defined specific expertise areas in Advanced endoscopy, hepatology, digestive oncology and clinical nutrition, training for the latter topic is lacking in the current hepatogastroenterology (HGE) curriculum. Given its relevance for HGE practice, and being at the core of gastrointestinal functioning, there is an obvious need for training in nutrition and related issues including the treatment of disease-related malnutrition and obesity and its associated metabolic derangements. This document aims to be a starting point for the integration of nutritional expertise in the HGE curriculum, allowing a central role in the management of malnutrition and obesity. We suggest minimum endpoints for nutritional knowledge and expertise in the standard curriculum and recommend a focus period of training in nutrition issues in order to produce well-trained HGE specialists. This article provides a road map for the organization of such a training program. We would highly welcome the World Gastroenterology Organisation, the European Board of Gastroenterology and Hepatology, the American Gastroenterology Association and other (inter)national Gastroenterology societies support the necessary certifications for this item in the HGE-curriculum
Median R squared and Inter Quartile Range (IQR) values for all comparisons.
<p>IQR values are indicated in brackets. Rows A and B indicate either intra-individual comparisons (A) or inter-individual comparisons (B).</p
Comparisons of microbiota profiles in the IBD outpatient group expressed as R squared.
<p>All comparisons have been done separately for <i>AFFV</i> group (left) and <i>Bacteroidetes</i> (right). Figures show comparisons of all profiles. Red dots represent comparisons of samples of the same subject (intra-subject correlation). Yellow box plots are based on all correlations, red box plots on intra-subject correlations only. A: Swabs taken by patients at home versus swabs taken by the physician at the polyclinic. B: Swabs versus fecal samples.</p