Phosphoinositide-dependent protein kinase-1 (PDK1)-independent activation of the protein kinase C substrate, protein kinase D

Phosphoinoisitide dependent kinase l (PDK1) is proposed to phosphorylate a key threonine residue within the catalytic domain of the protein kinase C (PKC) superfamily that controls the stability and catalytic competence of these kinases. Hence, in PDK1-null embryonic stem cells intracellular levels of PKCalpha, PKCbeta1, PKCgamma, and PKCepsilon are strikingly reduced. Although PDK1-null cells have reduced endogenous PKC levels they are not completely devoid of PKCs and the integrity of downstream PKC effector pathways in the absence of PDK1 has not been determined. In the present report, the PDK1 requirement for controlling the phosphorylation and activity of a well characterised substrate for PKCs, the serine kinase protein kinase D, has been examined. The data show that in embryonic stem cells and thymocytes loss of PDK1 does not prevent PKC-mediated phosphorylation and activation of protein kinase D. These results reveal that loss of PDK1 does not functionally inactivate all PKC-mediated signal transduction

Near Infrared Imaging of the Hubble Deep Field with The Keck Telescope

Two deep K-band ($2.2 \mu m$) images, with point-source detection limits of $K=25.2$ mag (one sigma), taken with the Keck Telescope in subfields of the Hubble Deep Field, are presented and analyzed. A sample of objects to K=24 mag is constructed and $V_{606}-I_{814}$ and $I_{814}-K$ colors are measured. By stacking visually selected objects, mean $I_{814}-K$ colors can be measured to very faint levels; the mean $I_{814}-K$ color is constant with apparent magnitude down to $V_{606}=28$ mag.Comment: Replaced with slightly revised source positions and corrected V-I magnitudes (which were incorrect in the Tables and Figure 5). 18 pages. The data are publicly available at http://www.cco.caltech.edu/~btsoifer/hdf.html along with a high-resolution version of Fig.

MESAS: Measuring the Emission of Stellar Atmospheres at Submm/mm wavelengths

In the early stages of planet formation, small dust grains grow to become mm sized particles in debris disks around stars. These disks can in principle be characterized by their emission at submillimeter and millimeter wavelengths. Determining both the occurrence and abundance of debris in unresolved circumstellar disks of A-type main-sequence stars requires that the stellar photospheric emission be accurately modeled. To better constrain the photospheric emission for such systems, we present observations of Sirius A, an A-type star with no known debris, from the JCMT, SMA, and VLA at 0.45, 0.85, 0.88, 1.3, 6.7, and 9.0 mm. We use these observations to inform a PHOENIX model of Sirius A's atmosphere. We find the model provides a good match to these data and can be used as a template for the submm/mm emission of other early A-type stars where unresolved debris may be present. The observations are part of an ongoing observational campaign entitled Measuring the Emission of Stellar Atmospheres at Submm/mm wavelengths (MESAS)Comment: 17 pages, 1 figure, Accepted to AJ on April 25th 201

Visualizing Spacetime Curvature via Frame-Drag Vortexes and Tidal Tendexes II. Stationary Black Holes

When one splits spacetime into space plus time, the Weyl curvature tensor (which equals the Riemann tensor in vacuum) splits into two spatial, symmetric, traceless tensors: the tidal field $E$, which produces tidal forces, and the frame-drag field $B$, which produces differential frame dragging. In recent papers, we and colleagues have introduced ways to visualize these two fields: tidal tendex lines (integral curves of the three eigenvector fields of $E$) and their tendicities (eigenvalues of these eigenvector fields); and the corresponding entities for the frame-drag field: frame-drag vortex lines and their vorticities. These entities fully characterize the vacuum Riemann tensor. In this paper, we compute and depict the tendex and vortex lines, and their tendicities and vorticities, outside the horizons of stationary (Schwarzschild and Kerr) black holes; and we introduce and depict the black holes' horizon tendicity and vorticity (the normal-normal components of $E$ and $B$ on the horizon). For Schwarzschild and Kerr black holes, the horizon tendicity is proportional to the horizon's intrinsic scalar curvature, and the horizon vorticity is proportional to an extrinsic scalar curvature. We show that, for horizon-penetrating time slices, all these entities ($E$, $B$, the tendex lines and vortex lines, the lines' tendicities and vorticities, and the horizon tendicities and vorticities) are affected only weakly by changes of slicing and changes of spatial coordinates, within those slicing and coordinate choices that are commonly used for black holes. [Abstract is abbreviated.]Comment: 19 pages, 7 figures, v2: Changed to reflect published version (changes made to color scales in Figs 5, 6, and 7 for consistent conventions). v3: Fixed Ref

Continuous-Valued Binary Decision Procedures

Conditions have been given elsewhere which guarantee that binary decision procedures have a simple structure. Here we show that a continuity requirement together with some weak algebraic regularity conditions ensures that a binary procedure is locally simple. Additional topological assumptions are given which require that the binary procedure is a simple game