21 research outputs found
The role of the testa during development and in establishment of dormancy of the legume seed
Timing of seed germination is one of the key steps in plant life cycles. It determines the beginning of plant growth in natural or agricultural ecosystems. In the wild, many seeds exhibit dormancy and will only germinate after exposure to certain environmental conditions. In contrast, crop seeds germinate as soon as they are imbibed usually at planting time. These domestication-triggered changes represent adaptations to cultivation and human harvesting. Germination is one of the common sets of traits recorded in different crops and termed the âdomestication syndrome.â Moreover, legume seed imbibition has a crucial role in cooking properties. Different seed dormancy classes exist among plant species. Physical dormancy (often called hardseededness), as found in legumes, involves the development of a water-impermeable seed coat, caused by the presence of phenolics- and suberin-impregnated layers of palisade cells. The dormancy release mechanism primarily involves seed responses to temperature changes in the habitat, resulting in testa permeability to water. The underlying genetic controls in legumes have not been identified yet. However, positive correlation was shown between phenolics content (e.g., pigmentation), the requirement for oxidation and the activity of catechol oxidase in relation to pea seed dormancy, while epicatechin levels showed a significant positive correlation with soybean hardseededness. myeloblastosis family of transcription factors, WD40 proteins and enzymes of the anthocyanin biosynthesis pathway were involved in seed testa color in soybean, pea and Medicago, but were not tested directly in relation to seed dormancy. These phenolic compounds play important roles in defense against pathogens, as well as affecting the nutritional quality of products, and because of their health benefits, they are of industrial and medicinal interest. In this review, we discuss the role of the testa in mediating legume seed germination, with a focus on structural and chemical aspects
Tag expression: Tagging with feeling
ABSTRACT In this paper we introduce tag expression, a novel form of preference elicitation that combines elements from tagging and rating systems. Tag expression enables users to apply affect to tags to indicate whether the tag describes a reason they like, dislike, or are neutral about a particular item. We present a user interface for applying affect to tags, as well as a technique for visualizing the overall community's affect. By analyzing 27,773 tag expressions from 553 users entered in a 3-month period, we empirically evaluate our design choices. We also present results of a survey of 97 users that explores users' motivations in tagging and measures user satisfaction with tag expression
The First HET Planet: A Companion to HD 37605
We report the first detection of a planetary-mass companion to a star using
the High Resolution Spectrograph (HRS) of the Hobby-Eberly Telescope (HET). The
HET-HRS now gives routine radial velocity precision of 2-3 m/s for high SNR
observations of quiescent stars. The planetary-mass companion to the metal-rich
K0V star HD37605 has an orbital period of 54.23 days, an orbital eccentricity
of 0.737, and a minimum mass of 2.84 Jupiter masses. The queue-scheduled
operation of the Hobby-Eberly Telescope enabled us to discovery of this
relatively short-period planet with a total observation time span of just two
orbital periods. The ability of queue-scheduled large-aperture telescopes to
respond quickly to interesting and important results demonstrates the power of
this new approach in searching for extra-solar planets as well as in other ares
of research requiring rapid response time critical observations.Comment: 4 Pages, 2 figures. Accepted in Astrophysical Journal Letters,
http://austral.as.utexas.edu/planets/hd37605/hd37605.htm
Effect of angiotensin-converting enzyme inhibitor and angiotensin receptor blocker initiation on organ support-free days in patients hospitalized with COVID-19
IMPORTANCE Overactivation of the renin-angiotensin system (RAS) may contribute to poor clinical outcomes in patients with COVID-19.
Objective To determine whether angiotensin-converting enzyme (ACE) inhibitor or angiotensin receptor blocker (ARB) initiation improves outcomes in patients hospitalized for COVID-19.
DESIGN, SETTING, AND PARTICIPANTS In an ongoing, adaptive platform randomized clinical trial, 721 critically ill and 58 nonâcritically ill hospitalized adults were randomized to receive an RAS inhibitor or control between March 16, 2021, and February 25, 2022, at 69 sites in 7 countries (final follow-up on June 1, 2022).
INTERVENTIONS Patients were randomized to receive open-label initiation of an ACE inhibitor (nâ=â257), ARB (nâ=â248), ARB in combination with DMX-200 (a chemokine receptor-2 inhibitor; nâ=â10), or no RAS inhibitor (control; nâ=â264) for up to 10 days.
MAIN OUTCOMES AND MEASURES The primary outcome was organ supportâfree days, a composite of hospital survival and days alive without cardiovascular or respiratory organ support through 21 days. The primary analysis was a bayesian cumulative logistic model. Odds ratios (ORs) greater than 1 represent improved outcomes.
RESULTS On February 25, 2022, enrollment was discontinued due to safety concerns. Among 679 critically ill patients with available primary outcome data, the median age was 56 years and 239 participants (35.2%) were women. Median (IQR) organ supportâfree days among critically ill patients was 10 (â1 to 16) in the ACE inhibitor group (nâ=â231), 8 (â1 to 17) in the ARB group (nâ=â217), and 12 (0 to 17) in the control group (nâ=â231) (median adjusted odds ratios of 0.77 [95% bayesian credible interval, 0.58-1.06] for improvement for ACE inhibitor and 0.76 [95% credible interval, 0.56-1.05] for ARB compared with control). The posterior probabilities that ACE inhibitors and ARBs worsened organ supportâfree days compared with control were 94.9% and 95.4%, respectively. Hospital survival occurred in 166 of 231 critically ill participants (71.9%) in the ACE inhibitor group, 152 of 217 (70.0%) in the ARB group, and 182 of 231 (78.8%) in the control group (posterior probabilities that ACE inhibitor and ARB worsened hospital survival compared with control were 95.3% and 98.1%, respectively).
CONCLUSIONS AND RELEVANCE In this trial, among critically ill adults with COVID-19, initiation of an ACE inhibitor or ARB did not improve, and likely worsened, clinical outcomes.
TRIAL REGISTRATION ClinicalTrials.gov Identifier: NCT0273570
Recommended from our members
Deriving the structure of pre-supernovae and delta Scuti stars using nonradial oscillations
This is the final report of a three-year Laboratory Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). The objective is to learn more about the internal structure of two classes of variable stars, by using the observational data afforded by their pulsation properties. The authors updated the one-dimensional computer codes to calculate the evolution and pulsation frequencies of representative delta Scuti and LBV models. They compared the observed pulsation properties with model predictions in an iterative process to find a model (or models) with interior structures that matched the observational constraints for several delta Scuti stars. They carried out nonlinear hydrodynamic modeling of LBV envelopes and proposed a mechanism for their periodic outbursts. Finally, they began validation of a two-dimensional stellar evolution code that will be used to investigate the effects of rotation and hydrodynamic instabilities on the interior structure of these stars
New Aspercryptins, Lipopeptide Natural Products, Revealed by HDAC Inhibition in Aspergillus nidulans
Large-Scale Metabolomics Reveals a Complex Response of <i>Aspergillus nidulans</i> to Epigenetic Perturbation
The microbial world
offers a rich source of bioactive compounds
for those able to sift through it. Technologies capable of quantitatively
detecting natural products while simultaneously identifying known
compounds would expedite the search for new pharmaceutical leads.
Prior efforts have targeted histone deacetylases in fungi to globally
activate the production of new secondary metabolites, yet no study
has directly assessed its effects with minimal bias at the metabolomic
level. Using untargeted metabolomics, we monitored changes in >1000
small molecules secreted from the model fungus, <i>Aspergillus
nidulans</i>, following genetic or chemical reductions in histone
deacetylase activity (HDACi). Through quantitative, differential analyses,
we found that nearly equal numbers of compounds were up- and down-regulated
by >100 fold. We detected products from both known and unknown
biosynthetic
pathways and discovered that <i>A. nidulans</i> is capable
of producing fellutamides, proteasome inhibitors whose expression
was induced by âŒ100 fold or greater upon HDACi. This work adds
momentum to an âomicsâ-driven resurgence in natural
products research, where direct detection replaces bioactivity as
the primary screen for new pharmacophores
New Aspercryptins, Lipopeptide Natural Products, Revealed by HDAC Inhibition in <i>Aspergillus nidulans</i>
Unlocking the biochemical
stores of fungi is key for developing
future pharmaceuticals. Through reduced expression of a critical histone
deacetylase in <i>Aspergillus nidulans</i>, increases of
up to 100-fold were observed in the levels of 15 new aspercryptins,
recently described lipopeptides with two noncanonical amino acids
derived from octanoic and dodecanoic acids. In addition to two NMR-verified
structures, MS/MS networking helped uncover an additional 13 aspercryptins.
The aspercryptins break the conventional structural orientation of
lipopeptides and appear âbackwardâ when compared to
known compounds of this class. We have also confirmed the 14-gene
aspercryptin biosynthetic gene cluster, which encodes two fatty acid
synthases and several enzymes to convert saturated octanoic and dodecanoic
acid to α-amino acids