1,127 research outputs found
Dimeric benzoboroxoles for targeted activity against Mycobacterium tuberculosis
Dimeric benzoboroxoles that are covalently linked by a short scaffold enhance selective anti-tubercular activity. These multimeric benzoboroxole compounds are capable of engaging the specific extracellular Mycobacterium tuberculosis glycans, do not lead to the evolution of resistance and bypass the need to cross the impermeable mycobacterial cell envelope barrier
Simultaneous real-time visible and infrared video with single-pixel detectors
Conventional cameras rely upon a pixelated sensor to provide spatial resolution. An alternative approach replaces the sensor with a pixelated transmission mask encoded with a series of binary patterns. Combining knowledge of the series of patterns and the associated filtered intensities, measured by single-pixel detectors, allows an image to be deduced through data inversion. In this work we extend the concept of a ‘single-pixel camera’ to provide continuous real-time video at 10 Hz , simultaneously in the visible and short-wave infrared, using an efficient computer algorithm. We demonstrate our camera for imaging through smoke, through a tinted screen, whilst performing compressive sampling and recovering high-resolution detail by arbitrarily controlling the pixel-binning of the masks. We anticipate real-time single-pixel video cameras to have considerable importance where pixelated sensors are limited, allowing for low-cost, non-visible imaging systems in applications such as night-vision, gas sensing and medical diagnostics
Thin film structural analysis using variable-period x-ray standing waves
Variable-period x-ray standing wave (VPXSW) studies have been carried out using 3 keV x rays and photoelectron detection. Two model surfaces have been used, a native SiO2 layer (20 Å thick) on bulk silicon, and a purpose-built multilayer surface comprising a chloroform/water marker layer (12 Å thick) on an ionic liquid spacer layer (211 Å thick) deposited on a SiO2/Si substrate at 90 K. By using photoelectron detection, both chemical and elemental sensitivity were achieved. The surfaces were modeled using dynamic x-ray scattering for x-ray intensity, and attenuation of photoelectrons transmitted through the layers, to produce simulations which accurately reproduced the experimental VPXSW measurements. VPXSW measurements made using the substrate, spacer layer, and marker layer photoelectron signatures produced consistent structural values. This work demonstrates that VPXSW can be used to determine chemically specific layer thicknesses within thick (≲300Å) surface structures composed of the light elements B, C, N, O, F, and Cl with an accuracy of 10 to 15 Å, perpendicular to the surface
Latent ice recrystallization inhibition activity in nonantifreeze proteins : Ca2+-activated plant lectins and cation-activated antimicrobial peptides
Organisms living in polar regions have evolved a series of antifreeze (glyco) proteins (AFGPs) to enable them to survive by modulating the structure of ice. These proteins have huge potential for use in cellular cryopreservation, ice-resistant surfaces, frozen food, and cryosurgery, but they are limited by their relatively low availability and questions regarding their mode of action. This has triggered the search for biomimetic materials capable of reproducing this function. The identification of new structures and sequences capable of inhibiting ice growth is crucial to aid our understanding of these proteins. Here, we show that plant c-type lectins, which have similar biological function to human c-type lectins (glycan recognition) but no sequence homology to AFPs, display calcium-dependent ice recrystallization inhibition (IRI) activity. This IRI activity can be switched on/off by changing the Ca2+ concentration. To show that more (nonantifreeze) proteins may exist with the potential to display IRI, a second motif was considered, amphipathicity. All known AFPs have defined hydrophobic/hydrophilic domains, rationalizing this choice. The cheap, and widely used, antimicrobial Nisin was found to have cation-dependent IRI activity, controlled by either acid or addition of histidine-binding ions such as zinc or nickel, which promote its amphipathic structure. These results demonstrate a new approach in the identification of antifreeze protein mimetic macromolecules and may help in the development of synthetic mimics of AFPs
Very Extended X-ray and H-alpha Emission in M82: Implications for the Superwind Phenomenon
We discuss the properties and implications of a 3.7x0.9 kpc region of
spatially-coincident X-ray and H-alpha emission about 11.6 kpc to the north of
the galaxy M82 previously discussed by Devine and Bally (1999). The PSPC X-ray
spectrum is fit by thermal plasma (kT=0.80+-0.17 keV) absorbed by only the
Galactic foreground column density. We evaluate the relationship of the
X-ray/H-alpha ridge to the M82 superwind. The main properties of the X-ray
emission can all be explained as being due to shock-heating driven as the
superwind encounters a massive ionized cloud in the halo of M82. This encounter
drives a slow shock into the cloud, which contributes to the excitation of the
observed H-alpha emission. At the same time, a fast bow-shock develops in the
superwind just upstream of the cloud, and this produces the observed X-ray
emission. This interpretation would imply that the superwind has an outflow
speed of roughly 800 km/s, consistent with indirect estimates based on its
general X-ray properties and the kinematics of the inner kpc-scale region of
H-alpha filaments. The gas in the M82 ridge is roughly two orders-of-magnitude
hotter than the minimum "escape temperature" at this radius, so this gas will
not be retained by M82.
(abridged)Comment: 24 pages (latex), 3 figures (2 gif files and one postscript),
accepted for publication in Part 1 of The Astrophysical Journa
A Randomized, Controlled, Supervised, Excerise Trial in Young Overweight Men and Women: The Midwest Exercise Trial II (MET2)
We evaluated weight loss response to 16 months of supervised exercise (45 minutes/d, 5 d/wk, 75% heart-rate-reserve) in sedentary, overweight/obese participants without energy restriction in the Midwest Exercise Trial (MET1). Results indicated men lost weight, women did not. The gender differences were associated with differences in the energy expenditure of exercise (EEEx) (men = 667 ± 116; women = 439 ± 88 kcal/session) when exercise was prescribed by frequency, intensity and duration. MET2 is a randomized control trial designed and powered to examine differences in weight loss and gender in response to EEEx for men and women of 400 or 600 kcal/session, 5d/wk, for 10 months without energy restriction. One hundred forty one participants will be randomized to 1 of 2 exercise groups or a non-exercise control. EEEx will be verified by indirect calorimetry monthly during the intervention. This study will evaluate: (1) the weight change response to two levels of EEEx versus non-exercise control; (2) gender differences in weight response to two levels of EEEx; (3) potential compensatory changes in energy intake and/or daily physical activity that may explain the observed weight changes. Results from this study may impact how exercise is prescribed for weight loss and prevention of weight regain and may clarify if men and women differ in response to exercise
Absorption-Line Probes of Gas and Dust in Galactic Superwinds
We discuss moderate resolution spectra of the NaD absorption-line in a sample
of 32 far-IR-bright starburst galaxies. In 18 cases, the line is produced
primarily by interstellar gas, and in 12 of these it is blueshifted by over 100
km/s relative to the galaxy systemic velocity. The absorption-line profiles in
these outflow sources span the range from near the galaxy systemic velocity to
a maximum blueshift of 400 to 600 km/s. The outflows occur in galaxies
systematically viewed more nearly face-on than the others. We therefore argue
that the absorbing material consists of ambient interstellar gas accelerated
along the minor axis of the galaxy by a hot starburst-driven superwind. The NaD
lines are optically-thick, but indirect arguments imply total Hydrogen column
densities of N_H = few X 10^{21} cm^{-2}. This implies that the superwind is
expelling matter at a rate comparable to the star-formation rate. This
outflowing material is very dusty: we find a strong correlation between the
depth of the NaD profile and the line-of-sight reddening (E(B-V) = 0.3 to 1
over regions several-to-ten kpc in size). The estimated terminal velocities of
superwinds inferred from these data and extant X-ray data are typically 400 to
800 km/s, are independent of the galaxy rotation speed, and are comparable to
(substantially exceed) the escape velocities for (dwarf) galaxies. The
resulting loss of metals can establish the mass-metallicity relation in
spheroids, produce the observed metallicity in the ICM, and enrich a general
IGM to 10 solar metallicity. If the outflowing dust grains survive their
journey into the IGM, their effect on observations of cosmologically-distant
objects is significant.Comment: 65 pages, including 16 figures. ApJ, in pres
Weight management by phone conference call: A comparision with a traditional face-to-face clinic. Rationale and design for a randomized equivalence trial
State-of-the-art treatment for weight management consists of a behavioral intervention to facilitate decreased energy intake and increased physical activity. These interventions are typically delivered face-to-face (FTF) by a health educator to a small group of participants. There are numerous barriers to participation in FTF clinics including availability, scheduling, the expense and time required to travel to the clinic site, and possible need for dependent care. Weight management clinics delivered by conference call have the potential to diminish or eliminate these barriers. The conference call approach may also reduce burden on providers, who could conduct clinic groups from almost any location without the expenses associated with maintaining FTF clinic space. A randomized trial will be conducted in 395 overweight/obese adults (BMI 25–39.9 kg/m2) to determine if weight loss (6 months) and weight maintenance (12 months) are equivalent between weight management interventions utilizing behavioral strategies and pre-packaged meals delivered by either a conference call or the traditional FTF approach. The primary outcome, body weight, will be assessed at baseline, 6, 12 and 18 months. Secondary outcomes including waist circumference, energy and macronutrient intake, and physical activity and will be assessed on the same schedule. In addition, a cost analysis and extensive process evaluation will be completed
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