総合臨床実習における実習成績の分析

本学で使用する実習成績評価の信頼性を検証するとともに、実習成績結果から学生の特徴について明確にすることを目的とした. 実習成績評価は中項目、小項目ともにCronbachの α信頼係数は高値を示し、本学で作成した成績表の全ての項目内容は信頼性、内的整合性が保たれていることがわかった. Klaster分析によって実習成績別に良好群、中間群、不良群にわけることができた. 学生の特徴として、良好群は全ての中項目で60点以上の成績であった. 中間群は基本的態度、対人関係、対象者のオリエンテーションなどコミュニケーション能力に関係する中項目では60点以上の成績となっていたが、評価に関係する中項目においては60点を下回る成績であった. 不良群では全ての中項目において60点を下回る成績であった.As well as verify the authenticity of the grading clinical practice used by the University, we have intended to clarify characteristics of student results from grading clinical practice. Cronbach\u27s reliability coefficient α indicates the threshold in both middle items, small items, grading clinical practice, he was found to be maintained reliability, and internal consistency of the contents of all the items of the league table that you created in the university. I can be divided into good group, middle group, the poor control group performance by training by analysis klaster. The characteristics of the students, was a good group performance of more than 60 items of all. Intermediary group in the field, but had become a grade of at least 60 points in that relate to communication skills, such as orientation basic attitudes, interpersonal relations, of the subject, in the field he was a grade below 60 points in the related to the evaluation. Bad group it was 60 points lower than the results of all the items

Fracture-induced amorphization of polycrystalline SiO2\mathrm{SiO_{2}} stishovite: a potential platform for toughening in ceramics

Silicon dioxide has eight stable crystalline phases at conditions of the Earth's rocky parts. Many metastable phases including amorphous phases have been known, which indicates the presence of large kinetic barriers. As a consequence, some crystalline silica phases transform to amorphous phases by bypassing the liquid via two different pathways. Here we show a new pathway, a fracture-induced amorphization of stishovite that is a high-pressure polymorph. The amorphization accompanies a huge volume expansion of ~100% and occurs in a thin layer whose thickness from the fracture surface is several tens of nanometers. Amorphous silica materials that look like strings or worms were observed on the fracture surfaces. The amount of amorphous silica near the fracture surfaces is positively correlated with indentation fracture toughness. This result indicates that the fracture-induced amorphization causes toughening of stishovite polycrystals. The fracture-induced solid-state amorphization may provide a potential platform for toughening in ceramics

Identification of an Antigenic Epitope in Helicobacter pylori Urease That Induces Neutralizing Antibody Production

We previously reported a mouse monoclonal antibody (MAb), termed L2, specific for Helicobacter pylori urease strongly inhibited its enzymatic activity. Here, to gain insight into how this antibody affects urease activity, the epitope that was recognized by the antibody was determined. By screening a panel of overlapping synthetic peptides covering the entire sequence of the two subunits (UreA and UreB), we identified a stretch of UreB-derived 19 amino acid (aa) residues (UB-33; aa 321 to 339, CHHLDKSIKEDVQFADSRI) that was specifically recognized by the L2 antibody. Further sequential amino acid deletion of the 19-mer peptide from either end allowed us to determine the minimal epitope as 8 amino acid residues (F8; SIKEDVQF) for L2 reactivity. This epitope appears to lie exactly on a short sequence which formed a flap over the active site of urease, suggesting that binding of the L2 antibody sterically inhibits access of urea, the substrate of urease. Finally, immunization of rabbits with either the 19-mer peptide or the 8-mer minimal epitope resulted in generation of antiurease antibodies that were capable of inhibiting the enzymatic activity. Since urease is critical for virulence of H. pylori, antigenic peptides that induce production of antibodies to inhibit its enzymatic activity may potentially be a useful tool as a vaccine for prevention and treatment of H. pylori infection