532 research outputs found
Validation of the Conservation and Robustness of Folding Initiation Sites in Evolutionarily Related Proteins
<Reports on the Sixteenth annual Meeting of the Tsukuba English Linguisic Society> Downstep in Japanese : Syntatic vs. Semantic Approach
It has been argued that the effect of downstep differently appears depending on the syntactic structure (Kubozono 1989, 1992 and Azuma 1992a, 1992b) or on the semantic information (Koori 1992a, 1992b) ..
Analyses of Sequences of (Ī²/Ī±) Barrel Proteins Based on the Inter-Residue Average Distance Statistics to Elucidate Folding Processes
Temperature dependence of energy gap of (GaAs)_n/(AlAs)_n Superlattices
Proceedings of SPI
Chronic partial unloading restores Ī²-adrenergic responsiveness and reverses receptor downregulation in failing rat hearts
ObjectivesMechanical unloading with a left ventricular assist device promotes āreverse remodeling,ā including restoration of Ī²-adrenergic receptor signaling and function. We compared the effects of partial unloading and complete unloading on Ī²-adrenergic responsiveness and gene expressions in failing rat hearts by use of heterotopic heartālung or heart transplantation models.MethodsFour weeks after ligation of the left anterior descending artery in Lewis rats, rats with heart failure were divided into 3 groups: infarcted hearts and lungs transplanted into the recipient rats (heart failureāpartial unloading, n = 8); infarcted hearts transplanted into the recipient rats (heart failureācomplete unloading, n = 7); infarcted (heart failure, n = 8) hearts without transplantation. Normal rats (n = 7) were used as controls. Papillary muscle function and gene expressions were studied at 2 or 4 weeks after transplantation.ResultsIn 2-week models, baseline developed tension of papillary muscles significantly increased in heart failureāpartial unloading and heart failureācomplete unloading compared with heart failure (0.15 Ā± 0.07 and 0.12 Ā± 0.05 g/mm2 vs 0.02 Ā± 0.01 g/mm2, P < .05). However, in 4-week models, they decreased to 0.11 Ā± 0.03 and 0.10 Ā± 0.03 g/mm2. In 4-week but not in 2-week models, the increase from baseline in baseline developed tension produced by Ī²-adrenergic stimulation (isoproterenol, 10ā8 and 10ā7 mol/L) was significantly increased in heart failureāpartial unloading compared with heart failureācomplete unloading and heart failure (P < .05). The mRNA expressions of brain natriuretic peptide and Ī²1- and Ī²2-adrenergic receptors were normalized in both 2- and 4-week models of heart failureāpartial unloading.ConclusionsChronic partial unloading but not complete unloading improved Ī²-adrenergic responsiveness and normalized brain natriuretic peptide and Ī²1- and Ī²2-adrenergic receptor mRNA expressions in the failing rat hearts
Identification of the catalytic subunit of cAMP-dependent protein kinase from the photosynthetic flagellate, Euglena gracilis Z11The nucleotide sequence data reported in this paper will appear in the DDBJ/EMBL/GenBank nucleotide sequence databases with the accession number AB021126.
AbstractA gene named epk2 that encodes the amino acid sequence of a protein kinase was identified from the photosynthetic flagellate, Euglena gracilis Z. Homology search and phylogenetic analysis revealed that the deduced amino acid sequence of epk2 is most similar to that of the catalytic subunit of cAMP-dependent protein kinase (PKA). Northern blot analysis showed that Euglena cells express a 1.4-kb transcript of this gene. When the EPK2 protein was coexpressed with the rat regulatory subunit of PKA in cultured mammalian cells, these two proteins were coimmunoprecipitated. The association of EPK2 and the rat regulatory subunit of PKA was not detected in the cell lysate incubated with cAMP. EPK2 immunoprecipitated from the transfected cells phosphorylated Kemptide, a synthetic peptide substrate for PKA, and the phosphorylation was inhibited by PKI, a PKA-selective protein kinase inhibitor. These results indicate that EPK2 is a PKA homologue in the photosynthetic flagellate, and this is the first evidence for the occurrence of the PKA catalytic subunit in photosynthetic organisms
Direct and indirect Transitions in (GaAs)_n/(AlAs)_n Superlattices with n=1-15
Proceedings of SPI
Generation of GeV protons from 1 PW laser interaction with near critical density targets
The propagation of ultra intense laser pulses through matter is connected
with the generation of strong moving magnetic fields in the propagation channel
as well as the formation of a thin ion filament along the axis of the channel.
Upon exiting the plasma the magnetic field displaces the electrons at the back
of the target, generating a quasistatic electric field that accelerates and
collimates ions from the filament. Two-dimensional Particle-in-Cell simulations
show that a 1 PW laser pulse tightly focused on a near-critical density target
is able to accelerate protons up to an energy of 1.3 GeV. Scaling laws and
optimal conditions for proton acceleration are established considering the
energy depletion of the laser pulse.Comment: 26 pages, 8 figure
Current management strategies for the pain of elderly patients with burning mouth syndrome : a critical review
Burning Mouth Syndrome (BMS), a chronic intraoral burning sensation or dysesthesia without clinically evident causes, is one of the most common medically unexplained oral symptoms/syndromes. Even though the clinical features of BMS have been astonishingly common and consistent throughout the world for hundreds of years, BMS remains an enigma and has evolved to more intractable condition. In fact, there is a large and growing number of elderly BMS patients for whom the disease is accompanied by systemic diseases, in addition to aging physical change, which makes the diagnosis and treatment of BMS more difficult. Because the biggest barrier preventing us from finding the core pathophysiology and best therapy for BMS seems to be its heterogeneity, this syndrome remains challenging for clinicians. In this review, we discuss currently hopeful management strategies, including central neuromodulators (Tricyclic Antidepressants - TCAs, Serotonin, and Norepinephrine Reuptake Inhibitors - SNRIs, Selective Serotonin Reuptake Inhibitors - SSRIs, Clonazepam) and solutions for applying non-pharmacology approaches. Moreover, we also emphasize the important role of patient education and anxiety management to improve the patientsā quality of life. A combination of optimized medication with a short-term supportive psychotherapeutic approach might be a useful solution
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