A MODIFICATION OF AN ESTIMATION METHOD OF THE NATURAL FREQUENCY OF A CUBE FORM MICRO SATELLITE

Micro satellites must survive severe mechanical conditions during their launch phase. One design requirement for rockets is the stiffness requirement, i.e. the natural frequencies requirement. In the early stages of satellite development, presumption of the natural frequency of a satellite may be difficult. The material used for the structure of many micro satellites is an aluminum alloy. The structure subsystem occupies a large portion of the satellite mass, and the elastic modulus of this aluminum alloy is larger than that of other subsystems. Therefore, the mechanical property of the aluminum alloy cannot be used to represent the mechanical property of the whole satellite. The density of an actual satellite differs from the density of the aluminum alloy. Therefore, when estimating the minimum natural frequency, the size and the elastic modules of an actual satellite structure must be used. When using an actual satellite structure, the estimated minimum natural frequencies of the lateral direction and the longitudinal direction during the ascent phase are in agreement with the measured values acquired by the vibration tests. In order to shorten a process of satellite development, this paper describes a practical method for estimating the natural frequency of a cube-shaped micro satellite This paper is a modified version of the previous paper [1] using new measurement results

Establishment of a New Cell Line(MTT-95) Showing Basophilic Differentiation from the Bone Marrow of a Patient with Acute Myelogenous Leukemia (M7)

A new myeloid cell line, MTT-95, was established from the bone marrow of a patient with acute myelogenous leukemia (AML, M7). MTT-95 cells differentiate into mature basophilic cells in culture medium with no chemical component or cytokine. Surface phenotypes were as follows: CD11b 79.3%, CD13 92.4%, CD33 99.8%, CD34 87.9%, CD41a 77.6% and HLA-DR 0.3%. MTT-95 cells were strongly positive for glycoprotein IIb/IIIa by immunohistochemical staining and revealed metachromatic granules. MTT-95 cells seem to possess characteristics of both megakaryocytes and basophils. These findings suggest that MTT-95 cells are basophil progenitors. MTT-95 cells might be useful in the study not only of the biological aspects of basophils, but also of the diversities of AML (M7).</p

Design, Implementation, and Operation of a Small Satellite Mission to Explore the Space Weather Effects in LEO

Ten-Koh is a 23.5 kg, low-cost satellite developed to conduct space environment effects research in low-Earth orbit (LEO). Ten-Koh was developed primarily by students of the Kyushu Institute of Technology (Kyutech) and launched on 29 October 2018 on-board HII-A rocket F40, as a piggyback payload of JAXA’s Greenhouse gas Observing Satellite (GOSAT-2). The satellite carries a double Langmuir probe, CMOS-based particle detectors and a Liulin spectrometer as main payloads. This paper reviews the design of the mission, specifies the exact hardware used, and outlines the implementation and operation phases of the project. This work is intended as a reference that other aspiring satellite developers may use to increase their chances of success. Such a reference is expected to be particularly useful to other university teams, which will likely face the same challenges as the Ten-Koh team at Kyutech. Various on-orbit failures of the satellite are also discussed here in order to help avoid them in future small spacecraft. Applicability of small satellites to conduct space-weather research is also illustrated on the Ten-Koh example, which carried out simultaneous measurements with JAXA’s ARASE satellite

Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes.

Research in autophagy continues to accelerate,(1) and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.(2,3) There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response

Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes

Research in autophagy continues to accelerate, and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose. There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.