Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana

Map-based gene cloning has played a key role in many genetic studies using the model plant,Arabidopsis thaliana. In the post-next generation sequencing era, identification of point mutations and their corresponding genes is increasingly becoming a powerful and important approach to define plant gene function. To perform initial mapping experiments efficiently on Arabidopsis mutants, enrichment of easy-to-use and reliable polymorphic DNA markers would be desirable. We present here a list of InDel polymorphic markers between Col-0 and Ler-0 accessions that can be detected in standard agarose gel electrophoresis

A giant adrenal pseudocyst presenting with right hypochondralgia and fever: a case report

<p>Abstract</p> <p>Introduction</p> <p>Adrenal pseudocysts are rare cystic masses that arise from the adrenal gland and which are usually non-functional and asymptomatic. Adrenal pseudocysts consist of a fibrous wall without an epithelial or endothelial lining. We report the case of a patient with a giant adrenal pseudocyst presenting with right hypochondralgia and high fever.</p> <p>Case presentation</p> <p>A 52-year-old Japanese man was admitted with right hypochondralgia and a chill. Abdominal computed tomography revealed a well-defined cystic mass measuring 19 cm which was located in the right adrenal region and the contents of which were not enhanced with contrast medium. Abdominal ultrasonography revealed a heterogeneously hypo-echoic lesion with a peripheral high-echoic rim. Serum hormonal levels were almost normal. Despite treatment with antibiotics, the high fever persisted. Based on these findings, we made a preoperative diagnosis of a right adrenal cyst with infection. However, the possibility of malignancy still remained. The patient underwent laparotomy and right adrenal cyst excision with partial hepatectomy in order to relieve the symptoms and to confirm an accurate diagnosis. Histological examination revealed an adrenal pseudocyst with infection. His condition improved soon after the operation.</p> <p>Conclusion</p> <p>We report a case of a giant adrenal pseudocyst with infection. Surgery is required for symptomatic cases in order to relieve the symptoms and in cases of uncertain diagnosis.</p

Porous V2O5/Carbon Nano-composites Electrodes for Rechargeable Power Sources with Large Capacity and High Power

Nanoporous composite electrodes for rechargeable power sources were successfully fabricated by coating porous carbon with thin V2O5 gel layers. Their electrochemical properties were demonstrated, which took after both lithium ion batteries and electrochemical double layer capacitors. The nanoporous V2O5/carbon composites exhibited large capacity of more than 100 mAh (g-composite).1 and good rate capability of 80% at 5.0 A (g-composite).1. The good performance is explained by electric double layer capacitance of large surface area and high rate lithium insertion to thin V2O5 gel layers.ナノダイナミクス国際シンポジウム 平成20年1月50日(木) 於長崎大学Nagasaki Symposium on Nano-Dynamics 2009 (NSND2009), January 27, 2026, Nagasaki University, Nagasaki, Japan, Poster Presentatio

Porous V2O5/Carbon Nano-composites Electrodes for Rechargeable Power Sources with Large Capacity and High Power

Nanoporous composite electrodes for rechargeable power sources were successfully fabricated by coating porous carbon with thin V2O5 gel layers. Their electrochemical properties were demonstrated, which took after both lithium ion batteries and electrochemical double layer capacitors. The nanoporous V2O5/carbon composites exhibited large capacity of more than 100 mAh (g-composite).1 and good rate capability of 80% at 5.0 A (g-composite).1. The good performance is explained by electric double layer capacitance of large surface area and high rate lithium insertion to thin V2O5 gel layers.ナノダイナミクス国際シンポジウム 平成20年1月50日(木) 於長崎大学Nagasaki Symposium on Nano-Dynamics 2009 (NSND2009), January 27, 2026, Nagasaki University, Nagasaki, Japan, Poster Presentatio

Application of Convolutional Neural Networks in the Diagnosis of Helicobacter pylori Infection Based on Endoscopic Images

Background and aims: The role of artificial intelligence in the diagnosis of Helicobacter pylori gastritis based on endoscopic images has not been evaluated. We constructed a convolutional neural network (CNN), and evaluated its ability to diagnose H. pylori infection. Methods: A 22-layer, deep CNN was pre-trained and fine-tuned on a dataset of 32, 208 images either positive or negative for H. pylori (first CNN). Another CNN was trained using images classified according to 8 anatomical locations (secondary CNN). A separate test data set (11, 481 images from 397 patients) was evaluated by the CNN, and 23 endoscopists, independently. Results: The sensitivity, specificity, accuracy, and diagnostic time were 81.9%, 83.4%, 83.1%, and 198 s, respectively, for the first CNN, and 88.9%, 87.4%, 87.7%, and 194 s, respectively, for the secondary CNN. These values for the 23 endoscopists were 79.0%, 83.2%, 82.4%, and 230 ± 65 min (85.2%, 89.3%, 88.6%, and 253 ± 92 min by 6 board-certified endoscopists), respectively. The secondary CNN had a significantly higher accuracy than endoscopists (by 5.3%; 95% CI, 0.3-10.2). Conclusion: H. pylori gastritis could be diagnosed based on endoscopic images using CNN with higher accuracy and in a considerably shorter time compared to manual diagnosis by endoscopists

Loss of function of an Arabidopsis homologue of JMJD6 suppresses the dwarf phenotype of acl5, a mutant defective in thermospermine biosynthesis

In Arabidopsis thaliana, the ACL5 gene encodes thermospermine synthase and its mutant, acl5, exhibits a dwarf phenotype with excessive xylem formation. Studies of suppressor mutants of acl5 reveal the involvement of thermospermine in enhancing mRNA translation of the SAC51 gene family. We show here that a mutant, sac59, which partially suppresses the acl5 phenotype, has a point mutation in JMJ22 encoding a D6-class Jumonji C protein (JMJD6). A T-DNA insertion allele, jmj22-2, also partially suppressed the acl5 phenotype while mutants of its closest two homologs JMJ21 and JMJ20 had no such effects, suggesting a unique role for JMJ22 in plant development. We found that mRNAs of the SAC51 family are more stabilized in acl5 jmj22-2 than in acl5

Mouse Models of Mutations and Variations in Autism Spectrum Disorder-Associated Genes: Mice Expressing Caps2/Cadps2 Copy Number and Alternative Splicing Variants

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by disturbances in interpersonal relationships and behavior. Although the prevalence of autism is high, effective treatments have not yet been identified. Recently, genome-wide association studies have identified many mutations or variations associated with ASD risk on many chromosome loci and genes. Identification of the biological roles of these mutations or variations is necessary to identify the mechanisms underlying ASD pathogenesis and to develop clinical treatments. At present, mice harboring genetic modifications of ASD-associated gene candidates are the best animal models to analyze hereditary factors involved in autism. In this report, the biological significance of ASD-associated genes is discussed by examining the phenotypes of mouse models with ASD-associated mutations or variations in mouse homologs, with a focus on mice harboring genetic modifications of the Caps2/Cadps2 (Ca2+-dependent activator protein for secretion 2) gene