Nature of the Unidentified TeV Source HESS J1614-518, Revealed by Suzaku and XMM-Newton Observations

We report on new Suzaku and XMM-Newton results concerning HESS J1614-518, which is one of the brightest extended TeV gamma-ray sources and has two regions with intense gamma-ray emission. We newly observed the south and center regions of HESS J1614-518 with Suzaku, since the north region, including the position of the 1st brightest peak of the TeV gamma-ray emission, has already been observed. No X-ray counterpart was found at the position of the 2nd brightest peak of the TeV gamma-ray emission; we estimated the upper limit of the X-ray flux to be 1.6 \times 10^{-13} erg cm^{-2} s^{-1} in the 2-10 keV band. The soft X-ray source Suzaku J1614-5152, which was found at the edge of the field of view in a previous observation, was also detected at the middle of HESS J1614-518. Analyzing the XMM-Newton archival data, we revealed that Suzaku J1614-5152 consists of multiple point sources. The X-ray spectrum of the brightest point source, XMMU J161406.0-515225, can be described by a power-law model with a photon index of Gamma = 5.2^{+0.6}_{-0.5}, or a blackbody model with temperature kT = 0.38^{+0.04}_{-0.04} keV. In the blackbody model, the hydrogen-equivalent column density is almost the same as that of the hard extended X-ray emission, Suzaku J1614-5141, which was found at the 1st peak position. If true, XMMU J161406.0-515225 may be physically related to Suzaku J1614-5141 and HESS J1614-518.Comment: Accepted for publication in PASJ Vol.63 No.SP

Cross-cultural validity of a dietary questionnaire for studies of dental caries risk in Japanese

Background: Diet is a major modifiable contributing factor in the etiology of dental caries. The purpose of this paper is to examine the reliability and cross-cultural validity of the Japanese version of the Food Frequency Questionnaire to assess dietary intake in relation to dental caries risk in Japanese. Methods: The 38-item Food Frequency Questionnaire, in which Japanese food items were added to increase content validity, was translated into Japanese, and administered to two samples. The first sample comprised 355 pregnant women with mean age of 29.2 +/- 4.2 years for the internal consistency and criterion validity analyses. Factor analysis (principal components with Varimax rotation) was used to determine dimensionality. The dietary cariogenicity score was calculated from the Food Frequency Questionnaire and used for the analyses. Salivary mutans streptococci level was used as a semi-quantitative assessment of dental caries risk and measured by Dentocult SM. Dentocult SM scores were compared with the dietary cariogenicity score computed from the Food Frequency Questionnaire to examine criterion validity, and assessed by Spearman's correlation coefficient (r(s)) and Kruskal-Wallis test. Test-retest reliability of the Food Frequency Questionnaire was assessed with a second sample of 25 adults with mean age of 34.0 +/- 3.0 years by using the intraclass correlation coefficient analysis. Results: The Japanese language version of the Food Frequency Questionnaire showed high test-retest reliability (ICC = 0.70) and good criterion validity assessed by relationship with salivary mutans streptococci levels (r(s) = 0.22; p < 0.001). Factor analysis revealed four subscales that construct the questionnaire (solid sugars, solid and starchy sugars, liquid and semisolid sugars, sticky and slowly dissolving sugars). Internal consistency were low to acceptable (Cronbach's alpha = 0.67 for the total scale, 0.46-0.61 for each subscale). Mean dietary cariogenicity scores were 50.8 +/- 19.5 in the first sample, 47.4 +/- 14.1, and 40.6 +/- 11.3 for the first and second administrations in the second sample. The distribution of Dentocult SM score was 6.8% (score = 0), 34.4% (score = 1), 39.4% (score = 2), and 19.4% (score = 3). Participants with higher scores were more likely to have higher dietary cariogenicity scores (p < 0.001; Kruskal-Wallis test). Conclusions: These results provide the preliminary evidence for the reliability and validity of the Japanese language Food Frequency Questionnaire

The movement of midwifery education and a future challenge

我が国における助産学教育は看護学教育の4年制化に伴い,大きな影響を受けている｡即ち,短大専攻科の4年制大学への移行により選択枠は減少し,講義および実習時間の短縮化を余儀なくされた｡さらに,専門学校の閉廃校も進んで助産師の養成数は減少し,その教育の質にも大きな問題を包含するようになった｡一方,少子化が進展し,地域社会や家庭における子育て力の低下等に伴う育児不安や児童虐待が大きな社会問題となっている｡このような社会情況の中で,助産師の果たすべき役割は益々増大し,助産師に対する期待は高まる一方である｡そこで,助産学教育の動向と取り巻く環境を概観することにより,その問題点を検討した｡その結果,1) 4年制看護学教育の中で保健師･助産師･看護師の3つの国家試験受験資格を得ることは容易ではないこと｡ 2) 社会の要請に応えうる人材育成のための専門的教育課程への移行が急務であることが示唆された。Recently, nursing education entered the four year college system; midwifery education in Japan has come to have serious problems, not only in the decline in the number of midwives training but also in the quality of the education due to abolition of the professional training school and the junior college's advanced midwifery training course. Then, lectures and the clinical training period have been shortened in midwifery education in four year colleges because of the crowded curriculum. On the other hand, the birth rate has been declining, and the anxiety of child care and child abuse are serious social issues in that child care in the community and at home is declining in Japan. The role of midwives has increased all the more, and midwives are indispensable for mothers, babies and families in such social conditions. Also, the problem was examined by surveying the environment surrounding the direction of midwifery education. It is not easy to get three state qualifications of public health nurse, midwife and nurse in the nursing education program within four years. It was suggested that change in the educational system of midwifery was a pressing need to train educated and skilled midwives to meet a social need

Nursing Education for a Gender-equal Society* - The Situation of Male Student Nurses -

題目:男女共同参画社会における看護教育―男子看護学生の動向について― 1975年の国際婦人年以来,カイロ宣言でのリプロダクテイブヘルス/ライツ,北京宣言等次々に男女差別の撤回と女性の自立をめざした運動が推進されてきた｡この女性のエンパワーメント運動の中で,我が国は労働基準法,男女雇用機会均等法,育児休業法等の制定および改正を経て,1999年6月｢男女共同参画社会基本法｣の施行に至った｡以来,国を挙げて男女共同参画社会の構築に取組んでいる｡看護界にあっては昭和23年に制定された保健婦助産婦看護婦法における看護職者は女子とされ,男子には国家免許を得る正式な資格が無かった｡やがて,法の改正とともに男性看護職者の進出が可能となってきた｡しかし,半世紀を経て,なお男性看護職者の占める割合は低く,看護界における男女共同参画の現状は進んでいるとはいい難い｡男性看護職者養成の沿革と現状を概観し,その問題点と課題を検討することとした｡Until quite recently, Japanese women had very few opportunities to become financially independent. However, women's rights and independence have been gradually promoted since 1975, the International Women's Year. The 1994 Cairo Conference on Population emphasized women's reproductive rights. Such international movements resulted in the enactment of the 1985 Equal Employment Opportunity Law and the 1999 Law for a Gender-Equal Society in Japan. On the other hand, nursing education had been offered for women only until it became possible for men to become nurses in 1968, when the 1948 law on nursing profession was amended. Almost half a century has passed, but the percentage of male nurses is still limited. There is still much to be done for achieving equality between male nurses and female nurses. We will overview the history of nursing education and discuss the problems that male nurses face. *Low No.78 of 1999 was established to promote genuine eguality between woman and me

Molecular mechanism of cerebral edema improvement via IL-1RA released from the stroke-unaffected hindlimb by treadmill exercise after cerebral infarction in rats

Cerebral edema following cerebral infarction can be severe and directly affect mortality and mobility. Exercise therapy after cerebral infarction is an effective therapeutic approach; however, the molecular mechanism remains unclear. Myokines such as interleukin-1 receptor antagonist (IL-1RA) are released during skeletal muscle contraction with effects on other organs. We hypothesized that myokine release during exercise might improve brain edema and confirmed the hypothesis using transient middle cerebral artery occlusion (tMCAO) model rats. Rats subjected to tMCAO were divided according to the severity of illness and further assigned to exercise and non-exercise groups. Treadmill exercises were performed at a speed of 2–8 m/min for 10 min from 1–6 days post-reperfusion after tMCAO. Exercise significantly reduced edema and neurological deficits in severely ill rats, with a reduction in aquaporin-4 (AQP4) expression in the ischemic core and increased blood IL-1RA release from the stroke-unaffected hindlimb muscle after tMCAO. Administration of IL-1RA into the lateral ventricles significantly reduced edema and AQP4 expression in the ischemic core. In conclusion, treadmill exercise performed in the early phase of stroke onset alleviated the decrease in blood IL-1RA following ischemic stroke. IL-1RA administration decreased astrocytic AQP4 expression in the ischemic core, suppressing brain edema.Gono R., Sugimoto K., Yang C., et al. Molecular mechanism of cerebral edema improvement via IL-1RA released from the stroke-unaffected hindlimb by treadmill exercise after cerebral infarction in rats. Journal of Cerebral Blood Flow and Metabolism, 43(5), 812-827. © 2023 SAGE Publishing. DOI: 10.1177/0271678X231151569

HER2 G776S mutation promotes oncogenic potential in colorectal cancer cells when accompanied by loss of APC function

Clinical cancer genome sequencing detects oncogenic variants that are potential targets for cancer treatment, but it also detects variants of unknown significance. These variants may interact with each other to influence tumor pathophysiology, however, such interactions have not been fully elucidated. Additionally, the effect of target therapy for those variants also unclarified. In this study, we investigated the biological functions of a HER2 mutation (G776S mutation) of unknown pathological significance, which was detected together with APC mutation by cancer genome sequencing of samples from a colorectal cancer (CRC) patient. Transfection of the HER2 G776S mutation alone slightly increased the kinase activity and phosphorylation of HER2 protein, but did not activate HER2 downstream signaling or alter the cell phenotype. On the other hand, the HER2 G776S mutation was shown to have strong oncogenic potential when loss of APC function was accompanied. We revealed that loss of APC function increased Wnt pathway activity but also increased RAS-GTP, which increased ERK phosphorylation triggered by HER2 G776S transfection. In addition, afatinib, a pan-HER tyrosine kinase inhibitor, suppressed tumor growth in xenografts derived from HER2 G776S-transfected CRC cells. These findings suggest that this HER2 mutation in CRC may be a potential therapeutic target

Research and Development of Information and Communication Technology-based Home Blood Pressure Monitoring from Morning to Nocturnal Hypertension

Asians have specific characteristics of hypertension (HTN) and its relationship with cardiovascular disease. The morning surge in blood pressure (BP) in Asians is more extended, and the association slope between higher BP and the risk for cardiovascular events is steeper in this population than in whites. Thus, 24-hour BP control including at night and in the morning is especially important for Asian patients with HTN. There are 3 components of “perfect 24-hour BP control”: the 24-hour BP level, adequate dipping of nocturnal BP (dipper type), and adequate BP variability such as the morning BP surge. The morning BP-guided approach using home BP monitoring (HBPM) is the first step toward perfect 24-hour BP control. After controlling morning HTN, nocturnal HTN is the second target. We have been developing HBPM that can measure nocturnal BP. First, we developed a semiautomatic HBPM device with the function of automatic fixed-interval BP measurement during sleep. In the J-HOP (Japan Morning Surge Home Blood Pressure) study, the largest nationwide home BP cohort, we successfully measured nocturnal home BP using this device with data memory, 3 times during sleep (2, 3, and 4 am), and found that nocturnal home BP is significantly correlated with organ damage independently of office and morning BP values. The second advance was the development of trigger nocturnal BP (TNP) monitoring with an added trigger function that initiates BP measurements when oxygen desaturation falls below a variable threshold continuously monitored by pulse oximetry. TNP can detect the specific nocturnal BP surges triggered by hypoxic episodes in patients with sleep apnea syndrome. We also added the lowest heart rate-trigger function to TNP to detect the “basal nocturnal BP,” which is determined by the circulating volume and structural cardiovascular system without any increase in sympathetic tonus. This double TNP is a novel concept for evaluating the pathogenic pressor mechanism of nocturnal BP. These data are now collected using an information and communication technology (ICT)-based monitoring system. The BP variability includes different time-phase variability from the shortest beat-by-beat, positional, diurnal, day-by-day, visit-to-visit, seasonal, and the longest yearly changes. The synergistic resonance of each type of BP variability would produce great dynamic BP surges, which trigger cardiovascular events. Thus, in the future, the management of HTN based on the simultaneous assessment of the resonance of all of the BP variability phenotypes using a wearable “surge” BP monitoring device with an ICT-based data analysis system will contribute to the ultimate individualized medication for cardiovascular disease

Reduction of 223Ra retention in the Large Intestine During Targeted Alpha Therapy with 223RaCl2 by Oral BaSO4 Administration in Mice

Background: Targeted alpha therapy with 223RaCl2 is used to treat skeletal metastases of hormone-refractory prostate cancer. The intravenous injection of 223RaCl2 causes gastrointestinal disorders such as nausea, abdominal discomfort, and diarrhea as frequent clinical adverse events caused by radiation. BaSO4 is known to display Ra2+ ion uptake in its structure and is clinically used as a contrast agent for X-ray imaging following oral administration. Here, we investigated the feasibility of a method to reduce 223Ra retention in the large intestine with BaSO4 by biodistribution studies in mice. Methods: 223RaCl2 biodistribution was examined in ddY mice after intravenous administration (10 kBq/mouse).BaSO4 (100 mg/mouse) was orally administered 1 h before 223RaCl2 injection. We also investigated the effect of laxative treatment on BaSO4 activity, since laxatives are clinically used with BaSO4 to avoid impaction in the large intestine. Results: BaSO4 significantly reduced 223Ra retention in the large intestine after 223RaCl2 injection in mice when compared with the control without BaSO4 administration (P &lt; 0.05). Excretion of 223Ra into the feces was significantly increased by BaSO4 administration (P &lt; 0.05). Laxative treatment did not affect BaSO4 activity in reducing 223Ra retention, although no additional effect of laxative treatment to 223Ra excretion was observed in mice. Conclusions. BaSO4 administration was effective in reducing 223Ra retention in the large intestine during 223RaCl2 therapy, and laxative treatment did not attenuate BaSO4 activity. This method could be useful in reducing adverse events caused by radiation exposure to the large intestine during 223RaCl2 therapy

SLITRK1-mediated noradrenergic projection suppression in the neonatal prefrontal cortex

SLITRK1 is an obsessive-compulsive disorder spectrum-disorders-ssociated gene that encodes a neuronal transmembrane protein. Here we show that SLITRK1 suppresses noradrenergic projections in the neonatal prefrontal cortex, and SLITRK1 functions are impaired by SLITRK1 mutations in patients with schizophrenia (S330A, a revertant of Homo sapiensspecific residue) and bipolar disorder (A444S). Slitrk1-KO newborns exhibit abnormal vocalizations, and their prefrontal cortices show excessive noradrenergic neurites and reduced Semaphorin3A expression, which suppresses noradrenergic neurite outgrowth in vitro. Slitrk1 can bind Dynamin1 and L1 family proteins (Neurofascin and L1CAM), as well as suppress Semaphorin3A-induced endocytosis. Neurofascin-binding kinetics is altered in S330A and A444S mutations. Consistent with the increased obsessive-compulsive disorder prevalence in males in childhood, the prefrontal cortex of male Slitrk1-KO newborns show increased noradrenaline levels, and serotonergic varicosity size. This study further elucidates the role of noradrenaline in controlling the development of the obsessive-compulsive disorder-related neural circuit