Pathogen-host reorganization during Chlamydia invasion revealed by cryo-electron tomography

Invasion of host cells is a key early event during bacterial infection, but the underlying pathogen-host interactions are yet to be fully visualised in three-dimensional detail. We have captured snapshots of the early stages of bacterial-mediated endocytosis in situ by exploiting the small size of chlamydial elementary bodies (EBs) for whole cell cryo-electron tomography. Chlamydiae are obligate intracellular bacteria that infect eukaryotic cells and cause sexually transmitted infections and trachoma, the leading cause of preventable blindness. We demonstrate that Chlamydia trachomatis LGV2 EBs are intrinsically polarised. One pole is characterised by a tubular inner membrane invagination, while the other exhibits asymmetric periplasmic expansion to accommodate an array of type III secretion systems (T3SSs). Strikingly, EBs orient with their T3SS-containing pole facing target cells, enabling the T3SSs to directly contact the cellular plasma membrane. This contact induces enveloping macropinosomes, actin-rich filopodia and phagocytic cups to zipper tightly around the internalising bacteria. Once encapsulated into tight early vacuoles, EB polarity and the T3SSs are lost. Our findings reveal previously undescribed structural transitions in both pathogen and host during the initial steps of chlamydial invasion

Generalized β\beta-conformal change and special Finsler spaces

In this paper, we investigate the change of Finslr metrics $$L(x,y) \to\bar{L}(x,y) = f(e^{\sigma(x)}L(x,y),\beta(x,y)),$$ which we refer to as a generalized $\beta$-conformal change. Under this change, we study some special Finsler spaces, namely, quasi C-reducible, semi C-reducible, C-reducible, $C_2$-like, $S_3$-like and $S_4$-like Finsler spaces. We also obtain the transformation of the T-tensor under this change and study some interesting special cases. We then impose a certain condition on the generalized $\beta$-conformal change, which we call the b-condition, and investigate the geometric consequences of such condition. Finally, we give the conditions under which a generalized $\beta$-conformal change is projective and generalize some known results in the literature.Comment: References added, some modifications are performed, LateX file, 24 page

Why Two Renormalization Groups are Better than One

The advantages of using more than one renormalization group (RG) in problems with more than one important length scale are discussed. It is shown that: i) using different RG's can lead to complementary information, i.e. what is very difficult to calculate with an RG based on one flow parameter may be much more accessible using another; ii) using more than one RG requires less physical input in order to describe via RG methods the theory as a function of its parameters; iii) using more than one RG allows one to solve problems with more than one diverging length scale. The above points are illustrated concretely in the context of both particle physics and statistical physics using the techniques of environmentally friendly renormalization. Specifically, finite temperature $\lambda\phi^4$ theory, an Ising-type system in a film geometry, an Ising-type system in a transverse magnetic field, the QCD coupling constant at finite temperature and the crossover between bulk and surface critical behaviour in a semi-infinite geometry are considered.Comment: 17 pages LaTex; to be published in the Proceedings of RG '96, Dubn

Variation of the broad X-ray iron line in MCG-6-30-15 during a flare

We report results on the broad iron emission line of the Seyfert galaxy MCG-6-30-15, obtained from the second long ASCA observation in 1997. The time-averaged profile of the broad line is very similar to that seen with ASCA in 1994, so confirming the detailed model fit then obtained. A bright flare is seen in the light curve, during which the continuum was soft. At that time the emission line peaks around 5 keV and most of its emission is shifted below 6 keV with no component detected at 6.4 keV (EW<60 eV). This can be interpreted as the result of an extraordinarily large gravitational redshift due to a dominant flare occurring very close to the black hole at a radius of <5m.Comment: 5 pages, accepted or publication in MNRAS Letter

Quantum multiparty key distribution protocol without use of entanglement

We propose a quantum key distribution (QKD) protocol that enables three parties agree at once on a shared common random bit string in presence of an eavesdropper without use of entanglement. We prove its unconditional security and analyze the key rate.Comment: 8 pages, no figur

Self-discharge characteristics of spacecraft nickel-cadmium cells at elevated temperatures

The effects of heat generation were determined in NiCd cells during high temperature storage on open circuits. The testing was designed to determine the extent to which thermal stability is a valid concern, at temperature of exposure (externally effected) between 40 and 120 C

Boltzmann Suppression of Interacting Heavy Particles

Matsumoto and Yoshimura have recently argued that the number density of heavy particles in a thermal bath is not necessarily Boltzmann-suppressed for T << M, as power law corrections may emerge at higher orders in perturbation theory. This fact might have important implications on the determination of WIMP relic densities. On the other hand, the definition of number densities in a interacting theory is not a straightforward procedure. It usually requires renormalization of composite operators and operator mixing, which obscure the physical interpretation of the computed thermal average. We propose a new definition for the thermal average of a composite operator, which does not require any new renormalization counterterm and is thus free from such ambiguities. Applying this definition to the model of Matsumoto and Yoshimura we find that it gives number densities which are Boltzmann-suppressed at any order in perturbation theory. We discuss also heavy particles which are unstable already at T=0, showing that power law corrections do in general emerge in this case.Comment: 7 pages, 5 figures. New section added, with the discussion of the case of an unstable heavy particle. Version to appear on Phys. Rev.

On the Bloch Theorem Concerning Spontaneous Electric Current

We study the Bloch theorem which states absence of the spontaneous current in interacting electron systems. This theorem is shown to be still applicable to the system with the magnetic field induced by the electric current. Application to the spontaneous surface current is also examined in detail. Our result excludes the possibility of the recently proposed $d$-wave superconductivity having the surface flow and finite total current.Comment: 12 pages, LaTeX, 3 Postscript figure