Component Analysis of Propolis from Papua New Guinea

Propolis is an aggregate of functional components found in plant resins and has been reported to exhibit valuable biological activities. This study investigated the components and antioxidant activity of propolis from Papua New Guinea. In component analysis, seven known compounds, 6-deoxyhaplopinol (1), 5-formylguaiacol (2), trans-caffeic acid (3), cis-caffeic acid (4), trans-ferulic acid (5), trans-p-coumaric acid (6), and L-kaempferitrin (7), were isolated and identified from Papua New Guinean propolis. The structure of 1 was confirmed by comparing the 13C NMR chemical shifts of the isolated and synthesized compounds. Based on component analysis, Papua New Guinean propolis may be a new type of propolis. The EtOH extracts of Papua New Guinean propolis exhibited antioxidant activity comparable to that of Baccharis and Populus propolis. This study demonstrated the potential of Papua New Guinean propolis in human health maintenance

HBO1-MLL interaction promotes AF4/ENL/P-TEFb-mediated leukemogenesis

白血病を引き起こすタンパク質間相互作用の発見. 京都大学プレスリリース. 2021-08-31.Leukemic oncoproteins cause uncontrolled self-renewal of hematopoietic progenitors by aberrant gene activation, eventually causing leukemia. However, the molecular mechanism underlying aberrant gene activation remains elusive. Here, we showed that leukemic MLL fusion proteins associate with the HBO1 histone acetyltransferase (HAT) complex through their trithorax homology domain 2 (THD2) in various human cell lines. MLL proteins associated with the HBO1 complex through multiple contacts mediated mainly by the ING4/5 and PHF16 subunits in a chromatin-bound context where histone H3 lysine 4 tri-methylation marks were present. Of the many MLL fusions, MLL-ELL particularly depended on the THD2-mediated association with the HBO1 complex for leukemic transformation. The C-terminal portion of ELL provided a binding platform for multiple factors including AF4, EAF1, and p53. MLL-ELL activated gene expression in murine hematopoietic progenitors by loading an AF4/ENL/P-TEFb (AEP) complex onto the target promoters wherein the HBO1 complex promoted the association with AEP complex over EAF1 and p53. Moreover, the NUP98-HBO1 fusion protein exerted its oncogenic properties via interaction with MLL but not its intrinsic HAT activity. Thus, the interaction between the HBO1 complex and MLL is an important nexus in leukemic transformation, which may serve as a therapeutic target for drug development

チイキ ト レンケイ シタ ソウゴウガタ チイキ スポーツ クラブ ニ オケル ガクセイ サンカガタ プログラム コウチク ノ トリクミ ミハマ スポーツ クラブ オ レイ トシテ

2012 年 9 月 23 日に， 日本福祉大学 （以下本学） と大学所在地である美浜町との連携による総合型地域スポーツクラブ 「みはまスポーツクラブ」 が開設された． クラブ開設の検討は 2008 年度より開始され， 検討会・準備委員会を経て 2010 年度よりプレ事業を行い， プログラム策定のための実践と検討を行ってきた． プレ事業の取り組みから得られた知見に加え， 「学生と地域とのふれあい」 「学生の学びを深める」 ことを中心とした学生の能力向上をミッションとし， 学生参加型プログラム構築の取り組みが行われた． この結果， 2012 年 9 月のクラブ開設時には， 学生が指導者として参加する小学生レクリエーション教室・ちびっこアジリティ教室がプログラムに加えられた． 本稿では， プログラム策定の経緯とプレ事業から得られた知見の報告を中心に， 学生が総合型地域スポーツクラブに参画する意義とについて考察を行う．On September 23rd, 2012, Nihon Fukushi University, in collaboration with the local town of Mihama, opened the Mihama Sports Club. Planning for the sports club began in 2008, and, through coordination with the preparatory committee, preliminary operations and program planning began in 2010. The insight gained from these preliminary initiatives led to the creation of a student program, which was designed to fulfill the club\u27s mission of strengthening the relationship between students and the local community and deepening students\u27 learning. As a result, when the club opened in September, recreational classes were added to give university students a chance to mentor elementary school students and toddlers. This report focuses on the knowledge gained through the program development process and preliminary operations, and, in doing so, considers the significance and role of student participation in community sports clubs