1,638 research outputs found
Probing hyperbolic polaritons using infrared attenuated total reflectance micro-spectroscopy
Hyperbolic polariton modes are highly appealing for a broad range of
applications in nanophotonics, including surfaced enhanced sensing,
sub-diffractional imaging and reconfigurable metasurfaces. Here we show that
attenuated total reflectance micro-spectroscopy (ATR) using standard
spectroscopic tools can launch hyperbolic polaritons in a Kretschmann-Raether
configuration. We measure multiple hyperbolic and dielectric modes within the
naturally hyperbolic material hexagonal boron nitride as a function of
different isotopic enrichments and flake thickness. This overcomes the
technical challenges of measurement approaches based on nanostructuring, or
scattering scanning nearfield optical microscopy. Ultimately, our ATR approach
allows us to compare the optical properties of small-scale materials prepared
by different techniques systematicallyComment: 13 pages 4 figure
Development of the morpholino gene knockdown technique in Fundulus heteroclitus : a tool for studying molecular mechanisms in an established environmental model
Author Posting. © Elsevier B.V., 2008. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Aquatic Toxicology 87 (2008): 289-295, doi:10.1016/j.aquatox.2008.02.010.A significant challenge in environmental toxicology is that many genetic and genomic tools available in laboratory models are not developed for commonly used environmental models. The Atlantic killifish (Fundulus heteroclitus) is one of the most studied teleost environmental models, yet few genetic or genomic tools have been developed for use in this species. The advancement of genetic and evolutionary toxicology will require that many of the tools developed in laboratory models be transferred into species more applicable to environmental toxicology. Antisense morpholino oligonucleotide (MO) gene knockdown technology has been widely utilized to study development in zebrafish and has been proven to be a powerful tool in toxicological investigations through direct manipulation of molecular pathways. To expand the utility of killifish as an environmental model, MO gene knockdown technology was adapted for use in Fundulus. Morpholino microinjection methods were altered to overcome the significant differences between these two species. Morpholino efficacy and functional duration were evaluated with molecular and phenotypic methods. A cytochrome P450-1A (CYP1A) MO was used to confirm effectiveness of the methodology. For CYP1A MO-injected embryos, a 70% reduction in CYP1A activity, a 86% reduction in total CYP1A protein, a significant increase in β-naphthoflavone-induced teratogenicity, and estimates of functional duration (50% reduction in activity 10 dpf, and 86% reduction in total protein 12 dpf) conclusively demonstrated that MO technologies can be used effectively in killifish and will likely be just as informative as they have been in zebrafish.This work was funded in part by the National Institute of Environmental Health Sciences through the Duke Superfund Basic Research Center (P42ES010356), the Boston University Superfund Basic Research Program (P42ES007381), and the Duke Integrated Toxicology and Environmental Health Program (ES-T32-0007031). Additional support was provided by a U.S. Environmental Protection Agency STAR fellowship awarded to C.R.F
WASP-4 Is Accelerating toward the Earth
The orbital period of the hot Jupiter WASP-4b appears to be decreasing at a rate of −8.64 ± 1.26 ms yr⁻¹, based on transit-timing measurements spanning 12 yr. Proposed explanations for the period change include tidal orbital decay, apsidal precession, and acceleration of the system along the line of sight. To investigate further, we performed new radial-velocity measurements and speckle imaging of WASP-4. The radial-velocity data show that the system is accelerating toward the Sun at a rate of −0.0422 ± 0.0028 m s⁻¹ day⁻¹. The associated Doppler effect should cause the apparent period to shrink at a rate of −5.94 ± 0.39 ms yr⁻¹, comparable to the observed rate. Thus, the observed change in the transit period is mostly or entirely produced by the line-of-sight acceleration of the system. This acceleration is probably caused by a wide-orbiting companion of mass 10–300 M_(Jup) and orbital distance 10–100 au, based on the magnitude of the radial-velocity trend and the nondetection of any companion in the speckle images. We expect that the orbital periods of one out of three hot Jupiters will change at rates similar to WASP-4b, based on the hot-Jupiter companion statistics of Knutson et al. Continued radial-velocity monitoring of hot Jupiters is therefore essential to distinguish the effects of tidal orbital decay or apsidal precession from line-of-sight acceleration
Multiple Projection Optical Diffusion Tomography with Plane Wave Illumination
We describe a new data collection scheme for optical diffusion tomography in
which plane wave illumination is combined with multiple projections in the slab
imaging geometry. Multiple projection measurements are performed by rotating
the slab around the sample. The advantage of the proposed method is that the
measured data can be much more easily fitted into the dynamic range of most
commonly used detectors. At the same time, multiple projections improve image
quality by mutually interchanging the depth and transverse directions, and the
scanned (detection) and integrated (illumination) surfaces. Inversion methods
are derived for image reconstructions with extremely large data sets. Numerical
simulations are performed for fixed and rotated slabs
NEOWISE Studies of Spectrophotometrically Classified Asteroids: Preliminary Results
The NEOWISE dataset offers the opportunity to study the variations in albedo
for asteroid classification schemes based on visible and near-infrared
observations for a large sample of minor planets. We have determined the
albedos for nearly 1900 asteroids classified by the Tholen, Bus and Bus-DeMeo
taxonomic classification schemes. We find that the S-complex spans a broad
range of bright albedos, partially overlapping the low albedo C-complex at
small sizes. As expected, the X-complex covers a wide range of albedos. The
multi-wavelength infrared coverage provided by NEOWISE allows determination of
the reflectivity at 3.4 and 4.6 m relative to the visible albedo. The
direct computation of the reflectivity at 3.4 and 4.6 m enables a new
means of comparing the various taxonomic classes. Although C, B, D and T
asteroids all have similarly low visible albedos, the D and T types can be
distinguished from the C and B types by examining their relative reflectance at
3.4 and 4.6 m. All of the albedo distributions are strongly affected by
selection biases against small, low albedo objects, as all objects selected for
taxonomic classification were chosen according to their visible light
brightness. Due to these strong selection biases, we are unable to determine
whether or not there are correlations between size, albedo and space
weathering. We argue that the current set of classified asteroids makes any
such correlations difficult to verify. A sample of taxonomically classified
asteroids drawn without significant albedo bias is needed in order to perform
such an analysis.Comment: Accepted to Ap
ASAS Light Curves of Intermediate Mass Eclipsing Binaries and the Parameters of HI Mon
We present a catalog of 56 candidate intermediate mass eclipsing binary
systems extracted from the 3rd data release of the All Sky Automated Survey. We
gather pertinent observational data and derive orbital properties, including
ephemerides, for these systems as a prelude to anticipated spectroscopic
observations. We find that 37 of the 56, or ~66% of the systems are not
identified in the Simbad Astronomical Database as known binaries. As a specific
example, we show spectroscopic data obtained for the system HI Mon (B0 V + B0.5
V) observed at key orbital phases based on the computed ephemeris, and we
present a combined spectroscopic and photometric solution for the system and
give stellar parameters for each component.Comment: 83 pages, 63 figure
Structure-Function Analysis of Escherichia coli DNA Helicase I Reveals Non-overlapping Transesterase and Helicase Domains
TraI (DNA helicase I) is an Escherichia coli F plasmid-encoded protein required for bacterial conjugative DNA transfer. The protein is a sequence-specific DNA transesterase that provides the site- and strand-specific nick required to initiate DNA strand transfer and a 5' to 3' DNA helicase that unwinds the F plasmid to provide the single-stranded DNA that is transferred from donor to recipient. Sequence comparisons with other transesterases and helicases suggest that these activities reside in the N- and C-terminal regions of TraI, respectively. Computer-assisted secondary structure probability analysis identified a potential interdomain region spanning residues 304-309. Proteins encoded by segments of traI, whose N or C terminus either flanked or coincided with this region, were purified and assessed for catalytic activity. Amino acids 1-306 contain the transesterase activity, whereas amino acids 309-1504 contain the helicase activity. The C-terminal 252 amino acids of the 1756-amino acid TraI protein are not required for either helicase or transesterase activity. Protein and nucleic acid sequence similarity searches indicate that the occurrence of both transesterase- and helicase-associated motifs in a conjugative DNA transfer initiator protein is rare. Only two examples (other than R100 plasmid TraI) were found: R388 plasmid TrwC and R46 plasmid (pKM101) TraH, belonging to the IncW and IncN groups of broad host range conjugative plasmids, respectively. The most significant structural difference between these proteins and TraI is that TraI contains an additional region of approximately 650 residues between the transesterase domain and the helicase-associated motifs. This region is required for helicase activity
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