Redox Parameters in Blood of Thyroid Cancer Patients After the Radioiodine Ablation

The radioactive iodine (I-131) ablation is a well-accepted treatment modality for differentiated thyroid cancer patients. Unfortunately, the radiation induces the oxidative stress and damages cells and tissues, simultaneously activating the mechanisms of antioxidative defense. Since the mechanisms of those processes are not completely known, we wanted to examine the changes in the most important reactive oxygen species and antioxidative components, as well as their correlation and significance for lipid peroxidation. Our results showed that the level of thiobarbituric acid reactive substances was increased during the first 30 days after the radiotherapy. Among antioxidant components, superoxide dismutase was increased in the 3rd and 30th day; catalase in 7th and reduced glutathione in 3rd and 7th day after the radiotherapy. As regards the prooxidants, the reduction of hydrogen peroxide (H2O2) was recorded in 7th and 30th day, and superoxide anion radical (O-2(center dot-)) was unchanged after the exposure to I-131. These results indicate that differentiated thyroid cancer patients are under constant oxidative stress despite the observed increase in antioxidative and reduction in prooxidative parameters. The understanding of these early processes is important since their progress determines the latter effects of I-131 therapy

Cadmium, Mercury and Lead in Hypericum perforatum L. collected in Western Serbia

Wild population of Hypericum perforatum growing in Western Serbia was analyzed for the content of important environmental pollutants cadmium, mercury and lead. Metal contents were determined by inductively coupled plasma optical emission spectrometry. Obtained results showed that levels of mercury and lead were under while cadmium concentrations exceeded limits recommended for medicinal plants. High levels of cadmium in investigated plants can be the result of soil enriched with cadmium as well as the ability of Hypericum perforatum to accumulate cadmium

Square-planar copper(II) complexes with tetradentate amido-carboxylate ligands. Crystal structure of Na2[Cu(obap)]2.H2O. Strain and spectral assignments of complexes

Novel N–N–N–O-type of tetradentate ligands H3obap (H3obap = oxamido-N-aminopropyl-N0-benzoic acid) and H3maeb (H3maeb = malamido-N-aminoethyl-N0-benzoic acid) and the corresponding square-planar copper(II) complexes have been prepared and characterized. The obap3 and maeb3 ligands coordinate to the copper(II) ion via four ligating atoms (three deprotonated atoms: one carboxylate oxygen and two deprotonated amide nitrogens; one amine nitrogen) with in-plane square chelation. A four coordinate, square-planar geometry has been established crystallographically for the binuclear Na2[Cu(obap)]2 Æ 2H2O complex. Structural data correlating the square-planar geometry of the [Cu(obap)] unit and an extensive strain analysis are discussed in relation to the information obtained for similar complexes. The infrared and electronic absorption spectra of the complexes are discussed in comparison to the related complexes of known geometries. Antibacterial activity of ligands and copper(II) complexes towards common Gram-negative and Gram-positive bacteria are reported as well

Structural, biological and computational study of oxamide derivative|СТРУКТУРНА, БИОЛОШКА И РАЧУНСКА ИПИТИВАЊА ДЕРИВАТА ОКСАМИДА

A dicarboxylato-diamide-type compound 2,2'-[(1,2-dioxoethane-1,2--diyl)diimino]dibenzoicacid (H(4)obbz) (1) was synthesized and characterized. The crystal structure of K(2)H(2)obbz center dot 2H(2)O (2) was determined by X-ray diffract-tion analysis. The cytotoxic activities of the compounds were tested against four different cancer cell lines MCF-7, A549, HT-29, HeLa and a human nor-mal cell line MRC-5. The results indicate reasonable dose-dependent cytotox-icity of the ligands that show selectivity against the tested carcinoma and healthy cell lines. Flow cytometric analysis and fluorescence microscopy showed that the most active compound, H(4)obbz, induced apoptosis and G0/G1 cell cycle arrest, indicating blockage of DNA synthesis as a possible mechanism that trig-gers apoptosis. Docking and molecular dynamics simulations gave similar res-ponses regarding interactions (binding) between their ligands and chaperon Grp78. The MMGBSA determined Delta G binding energies were in the range from -104 to -140 kJ mol(-1)